Enhancement of the Upconversion Emission by Visible-to-Near-Infrared Fluorescent Graphene Quantum Dots for miRNA Detection

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dc.contributor.authorLaurenti, Marco
dc.contributor.authorPaez-Pérez, Miguel
dc.contributor.authorAlgarra González, Manuel
dc.contributor.authorAlonso Cristobal, Paulino
dc.contributor.authorLópez Cabarcos, Enrique
dc.contributor.authorMéndez González, Diego
dc.contributor.authorRubio Retama, Benito Jorge
dc.date.accessioned2024-02-12T08:53:45Z
dc.date.available2024-02-12T08:53:45Z
dc.date.issued2016-05-25
dc.description.abstractWe developed a sensor for the detection of specific microRNA (miRNA) sequences that was based on graphene quantum dots (GQDs) and ssDNA-UCNP@SiO2. The proposed sensor exploits the interaction between the sp2 carbon atoms of the GQD, mainly π–π stacking, and the DNA nucleobases anchored on the upconversion nanoparticles (UCNPs). This interaction brings the GQD to the surface of the ssDNA-UCNP@SiO2 system, enhancing the upconversion emission. On the other hand, hybridization of the single-stranded DNA (ssDNA) chains anchored on the nanoparticles with their complementary miRNA sequences blocks the capacity of the UCNPs to interact with the GQD through π–π stacking. That gives as result a reduction of the fluorescent enhancement, which is dependent on the concentration of miRNA sequences. This effect was used to create a sensor for miRNA sequences with a detection limit of 10 fM.
dc.description.departmentDepto. de Química en Ciencias Farmacéuticas
dc.description.facultyFac. de Farmacia
dc.description.refereedTRUE
dc.description.sponsorshipBill & Melinda Gates Foundation
dc.description.sponsorshipMinisterio de Economía, Comercio y Empresa (España)
dc.description.statuspub
dc.identifier.citationLaurenti M, Paez-Perez M, Algarra M, Alonso-Cristobal P, Lopez-Cabarcos E, Mendez-Gonzalez D, et al. Enhancement of the Upconversion Emission by Visible-to-Near-Infrared Fluorescent Graphene Quantum Dots for miRNA Detection. ACS Appl Mater Interfaces 2016;8:12644–51. https://doi.org/10.1021/acsami.6b02361.
dc.identifier.doi10.1021/acsami.6b02361
dc.identifier.issn1944-8244
dc.identifier.officialurlhttps://doi.org/10.1021/acsami.6b02361
dc.identifier.urihttps://hdl.handle.net/20.500.14352/101129
dc.journal.titleACS Applied Materials & Interfaces
dc.language.isoeng
dc.page.final12651
dc.page.initial12644
dc.publisherAmerican Chemical Society
dc.relation.projectIDinfo:eu-repo/grantAgreement/OPP1128411
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/MAT2014-55065R
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu615:54
dc.subject.cdu615.31
dc.subject.keywordUpconversion
dc.subject.keywordnanoparticles
dc.subject.keywordgraphene quantum dots
dc.subject.keywordenergy transfer
dc.subject.keywordmiRNA sensor
dc.subject.ucmCiencias Biomédicas
dc.subject.ucmQuímica
dc.subject.unesco23 Química
dc.subject.unesco2307 Química Física
dc.titleEnhancement of the Upconversion Emission by Visible-to-Near-Infrared Fluorescent Graphene Quantum Dots for miRNA Detection
dc.typejournal article
dc.type.hasVersionAM
dc.volume.number8
dspace.entity.typePublication
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relation.isAuthorOfPublicationa8cb17b3-6f5d-404c-b1d3-05749a92a5f3
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relation.isAuthorOfPublicatione472b936-73b0-45a5-b92a-7b3be8543cc8
relation.isAuthorOfPublication.latestForDiscovery3f84ff29-c7f1-4213-82be-e2bff4127a45
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