Person: Rosales Conrado, Noelia
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First Name
Noelia
Last Name
Rosales Conrado
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Químicas
Department
Química Analítica
Area
Química Analítica
Identifiers
12 results
Search Results
Now showing 1 - 10 of 12
Item Neuroprotective Action of Multitarget 7-Aminophenanthridin-6(5H)-one Derivatives against Metal-Induced Cell Death and Oxidative Stress in SN56 Cells(ACS Chemical Neuroscience, 2021) Moyano-Cires Ivanoff, Paula Viviana; Vicente Zurdo, David; Blázquez-Barbadillo, Cristina; Menéndez Ramos, José Carlos; González Matilla, Juan Francisco; Rosales Conrado, Noelia; Pino Sans, Javier DelNeurodegenerative diseases have been associated with brain metal accumulation, which produces oxidative stress (OS), matrix metalloproteinases (MMPs) induction, and neuronal cell death. Several metals have been reported to downregulate both the nuclear factor erythroid 2-related factor 2 (Nrf2) pathway and the antioxidant enzymes regulated by it, mediating OS induction and neurodegeneration. Among a recently discovered family of multitarget 7-amino-phenanthridin-6-one derivatives (APH) the most promising compounds were tested against metal-induced cell death and OS in SN56 cells. These compounds, designed to have chelating activity, are known to inhibit some MMPs and to present antioxidant and neuroprotective effects against hydrogen peroxide treatment to SN56 neuronal cells. However, the mechanisms that mediate this protective effect are not fully understood. The obtained results show that compounds APH1, APH2, APH3, APH4, and APH5 were only able to chelate iron and copper ions among all metals studied and that APH3, APH4, and APH5 were also able to chelate mercury ion. However, none of them was able to chelate zinc, cadmium, and aluminum, thus exhibiting selective chelating activity that can be partly responsible for their neuroprotective action. Otherwise, our results indicate that their antioxidant effect is mediated through induction of the Nrf2 pathway that leads to overexpression of antioxidant enzymes. Finally, these compounds exhibited neuroprotective effects, reversing partially or completely the cytotoxic effects induced by the metals studied depending on the compound used. APH4 was the most effective and safe compound.- Project number: 71
Item Diseño de un e-portfolio para la adquisición de competencias transversales en Educación para la Sostenibilidad usando las TIC(2021) Peña Martínez, Juan; Fernández Lázaro, Gloria; Pérez López, Raquel; Ortiz Iniesta, María Jesús; Lara Caballero, Ana; Rosales Conrado, Noelia; Andrés Casado, NuriaDiseño de un portafolio digital, incluido sistema de rúbrica, para integrar la Educación para la Sostenibilidad en la enseñanza-aprendizaje de las ciencias experimentales favoreciendo el empleo de las TIC y competencias como el razonamiento reflexivo. Item Clase invertida de espectroscopía: una experiencia en el marco de un Programa de Excelencia para Bachillerato(Educación Química, 2020) Gómez Gómez, Beatriz; Peña Martínez, Juan; Rosales Conrado, NoeliaEn el presente trabajo se describe una experiencia didáctica en un curso de Bachillerato, perteneciente al Programa de Excelencia, de un centro escolar de la Comunidad de Madrid (España). Mediante un modelo de clase invertida o flipped classroom (de su traducción en inglés), se abordaron los fundamentos relacionados con las técnicas de análisis espectroscópico y se realizaron actividades experimentales como la preparación de un espectroscopio “casero”. Tras la intervención, se llevó a cabo una encuesta de satisfacción para conocer la percepción de los estudiantes sobre las actividades realizadas y sobre el modelo de enseñanza-aprendizaje aplicado. Los alumnos han valorado positivamente la nueva metodología, si bien, su aplicación ha generado un cierto desconcierto que puede generar dudas a la hora de aplicar el enfoque de clase invertida a otras asignaturas.Item Integration of untargeted and targeted mass spectrometry-based metabolomics provides novel insights into the potential toxicity associated to surfynol(Food and Chemical Toxicology, 2020) Garcia-Calvo, Estefania; Machuca Marcos, Andrés; Nerin, Cristina; Rosales Conrado, Noelia; Anunciacao, Daniela S; Luque García, José LuisThe intake of toxic compounds through the diet as a result of migration processes from food packaging is of increasing concern. It has been shown that the surfactant commercially known as surfynol, which is commonly used in food-contact materials, is capable of migrating from multilayer containers into the food, reaching potentially harmful concentration levels. In the present study, the integration of an untargeted and a targeted metabolomics approach has been carried out using NTERA-2 germinal cells as in-vitro model, to make further progress in elucidating the molecular mechanisms associated with the toxicity of surfynol. This study has allowed the identification of different altered metabolites mainly related with energy-acquiring, cell development and cellular defense mechanisms. While glutamine, L-threonine, propanoate, octadecanoate and carbamate were found at higher concentrations in cells exposed tu surfynol, L-valine, oxalate, phosphate, phenylalanine and myoinositol were found inhibited. Additionally, concentrations of ATP, ADP and NAD+ were found significantly inhibited, supporting the idea that surfynol induces glycolysis inactivation. The results obtained strengthen the evidence of the toxicity associated to surfynol; therefore, reinforcing the need for a more comprehensive study on the viability of its use in food packaging.Item Aprendizaje de las reacciones químicas en Educación Secundaria a través de actividades cooperativas(Educación Química, 2021) Peña Martínez, Juan; Pérez López, Raquel; Rosales Conrado, NoeliaEn el presente trabajo se propone una secuencia didáctica para la enseñanza de las reacciones químicas en educación secundaria, que incluye dos actividades diferentes de aprendizaje cooperativo. La primera, para trabajar la diferencia entre cambio físico y cambio químico y, la segunda, para consolidar la parte experimental que también se ha llevado a cabo en el laboratorio. Un total de 43 estudiantes valoraron la experiencia y la metodología empleada mediante un cuestionario. Los resultados muestran que aproximadamente un 80% están a favor de emplear el modelo de aprendizaje con grupos cooperativos. No obstante, un 30% de los estudiantes señalan que hay que asegurar que todos los miembros de los equipos participen y realicen el trabajo asignado. La metodología cooperativa se podría emplear en la enseñanza de las reacciones químicas.Item Bioactive polyphenols from Ranunculus macrophyllus Desf. Roots: quantification, identification and antioxidant activity(South African Journal of Botany, 2020) Deghima, Amirouche; Righi, Nadjat; Rosales Conrado, Noelia; León González, María Eugenia De; Gómez Mejía, Esther; Madrid Albarrán, María Yolanda; Baali, Faiza; Bedjou, FatihaRanunculus macrophyllus Desf. is an Algerian medicinal plant whose roots are used in traditional medicine to cure feminine infertility and other diseases, however there are no studies regarding its phytochemistry and biological activities. The aim of this work is to study the phytochemical composition and antioxidant activity of different solvents fractions from the roots of Ranunculus macrophyllus Desf. Spectrophotometric and chromatographic methods were used to study the phytochemical composition; while antiradical, iron chelating ability, reducing power and lipid peroxidation were studied in-vitro. The ethyl acetate fraction showed the highest values of total phenolic compounds (271.0 ± 0.2 µg GAE/mg dry extract (d.e), flavonols (24 ± 5 µg RE/ mg d.e) and condensed tannins (129 ± 10 µg CE/mg d.e), while the hexane fraction contained the highest amount of triterpenoids (91 ± 7 µg UAE/mg d.e). The highest radical scavenging ability was recorded for the ethyl acetate fraction against DPPH (IC50 = 3.7 ± 0.1 µg/mL) and ABTS (IC50 = 81 ± 3 µg/mL) whereas the hexane fraction had the best hydrogen peroxide radical scavenging (IC50 = 380 ± 4 µg/mL). The ethyl acetate fraction had the best total antioxidant capacity (TAC = 361 ± 1 µAAE/ mg extract) and reducing power (310 ± 2 µAAE/mg extract). The β-carotene bleaching was inhibited at high rate even after 24 h by the ethyl acetate fraction (81.0 ± 0.5 %). All activities were correlated with the polyphenolic content of the fractions. Capillary LC-DAD and LC-MS/MS analysis of ethyl acetate fraction revealed high amounts of gallic acid (9.3 ± 0.6 mg/g d.e), dihydroxybenzoic acid (8.1 ± 0.2 mg/g d.e) and hesperidin (5.9 ± 0.6 mg/g d.e). With such high amounts of polyphenols and strong antioxidant activity Ranunculus macrophyllus Desf. roots could have a potential use in pharmaceutical and nutraceutical industries.- Project number: 181
Item Reorientación de la didáctica de la Química para la mejora de la formación inicial de maestros en el ámbito de las competencias profesionales en Educación para la Sostenibilidad(2020) Peña Martínez, Juan; Fernández Lázaro, Gloria; Pérez López, Raquel; Ortiz Iniesta, María Jesús; Rosales Conrado, Noelia; Martínez del Campo, Teresa; Marín Espinosa, SergioEn el presente proyecto se ha elaborado una guía de orientación para la enseñanza-aprendizaje de la Química que incluye contenidos teóricos, cuestionarios y una propuesta de actividades dirigidas a promover la adquisición de competencias en Educación para la Sostenibilidad. Se emplearon diferentes metodologías en el desarrollo de las actividades: enseñanza basada en la indagación, Design For Change (design thinking), gamificación y aprendizaje basado en proyectos (diseño de un cultivo acuapónico). Los estudiantes crearon un portafolio digital para recopilar todos los resultados de las actividades y sus reflexiones personales. Item Ability of selenium species to inhibit metal-induced Aβ aggregation involved in the development of Alzheimer’s disease(Analytical and Bioanalytical Chemistry, 2020) Vicente Zurdo, David; Romero Sánchez, Iván; Rosales Conrado, Noelia; León González, María Eugenia De; Madrid Albarrán, María Yolanda; Springer HeidelbergExtracellular accumulation of amyloid beta peptide (Aβ) is believed to be one of the main factors responsible for neurodegeneration in Alzheimer’s disease (AD). Metals could induce Aβ aggregation, by their redox activity or binding properties to amyloid β fibrils, leading to their accumulation and deposition outside neurons. For this reason, metal chelation may have an acknowledged part to play in AD prevention and treatment. In the current work, the role of different selenium species, including selenium nanoparticles, in Aβ aggregation, was studied by evaluating their metal-chelating properties and their ability both to inhibit metal-induced Aβ1–42 aggregation fibrils and to disaggregate them once formed. Transition biometals such as Fe(II), Cu(II), and Zn(II) at 50 μM were selected to establish the in vitro models. The DPPH assay was used to determine the antioxidant capacity of the evaluated selenium species. Selenium nanoparticles stabilized with chitosan (Ch-SeNPs) and with both chitosan and chlorogenic acid polyphenol (CGA@ChSeNPs) showed the highest antioxidant properties with EC50 of 0.9 and 0.07 mM, respectively. UV–Vis and d1(UV–Vis) spectra also revealed that selenium species, in particular selenomethionine (SeMet), were able to interact with metals. Regarding Aβ1–42 incubation experiments, Fe(II), Cu(II), and Zn(II) induced Aβ aggregation, in a similar way to most of the evaluated selenium species. However, Ch-SeNPs produced a high inhibition of metal-induced Aβ aggregation, as well as a high disaggregation capacity of Aβ fibrils in both the presence and absence of biometals, in addition to reducing the length and width (20% of reduction in the presence of Zn(II)) of the generated Aβ fibrils.Item Anti-inflammatory activity of ethyl acetate and n-butanol extracts from Ranunculus macrophyllus Desf. and their phenolic profile(Journal of Ethnopharmacology, 2021) Deghima, Amirouche ; Righi, Nadjat; Rosales Conrado, Noelia; León González, María Eugenia De; Baali, Faiza; Gómez Mejía, Esther; Madrid Albarrán, María Yolanda; Bedjou, FatihaEthnopharmacological relevance: The members of the genus Ranunculus have counter-irritating properties and thus, they are traditionally used for treating anti-inflammatory disorders and other skin conditions. Ranunculus macrophyllus Desf. is a wild medicinal plant growing in Algeria and traditionally used to treat some cutaneous skin disorders. Aim: The aim of this study was to characterize the composition of the ethyl acetate and n-butanol extracts from Ranunculus macrophyllus Desf. as well as to elucidate and to compare their effect against acute skin inflammation. Moreover, both the antioxidant activity and the acute toxicity of the plant extracts were also studied. Materials and methods: Spectrophotometric and chromatographic methods were employed to identify and quantify phenolic compounds and triterpenoids from R. macrophyllus Desf. fractions. The antioxidant activity was estimated using the phosphomolebdenum, DPPH, reducing power and β-carotene bleaching assays. The ethyl acetate and n-butanol extracts were screened for their anti-inflammatory activities using ex-vivo membrane stabilizing assays and in-vivo acute skin inflammation model. Results: Ethyl acetate fraction showed the highest amounts of total phenolic compounds (413 ± 4 μg GAE/mg extract) and triterpenoids (70.4 ± 1.8 μg UAE/mg extract). Rutin, hesperidin, myricetin and kaempferol were the major compounds identified in the different fractions. Ethyl acetate fraction exhibited strong DPPH• radical scavenging ability (IC50 1.6 ± 0.2 μg/mL), high total antioxidant capacity (447 ± 7 μg AAE/mg extract) and reducing power (514 ± 8 μg AAE/mg extract). Ethyl acetate fraction inhibited (73.4 ± 0.3) % of linoleic acid peroxidation. Ethyl acetate and n-butanol fractions did not have any visible toxicity at 2000 mg/kg and presented excellent membrane stabilizing ability. The inhibition of xylene induced ear inflammation was (38 ± 4) % and (46 ± 1) % for RM-B and RM-EA, respectively. Conclusions: The high content of both phenolic compounds and triterpenoids combined with the remarkable antiinflammatory effect and antioxidant activity of ethyl acetate and n-butanol extracts from R. macrophyllus Desf. support the wide spread use of this traditional plant on some skin disorders (inflammatory skin disorders).Item Valorisation of the green waste parts from large-leaved buttercup (Ranunculus macrophyllus Desf.): phenolic profile and health promoting effects study(Waste and biomass valorization, 2020) Deghima, Amirouche; Righi, Nadjat; Rosales Conrado, Noelia; León González, María Eugenia De; Baali, Faiza; Gómez Mejía, Esther; Madrid Albarrán, María Yolanda; Bedjou, Fatiha; SpringerDue to the extensive use of Ranunculus macrophyllus Desf. roots for medicinal purposes, most of the leafy green parts are just wasted. The aim of this work is to valorize the leafy green parts and promote their application in different modern industries. Methods For this purpose, we studied the phenolic profile of R. macrophyllus Desf. (RM-B) using chromatographic and spectrophotometric methods and we tested the in-vitro antioxidant activity and the in-vivo effect of RM-B on plasma and liver antioxidant statuts. Results RM-B contained high amounts of polyphenols (675 mg GAE/100 g dry weigh dw) and flavonoids (105 mg QE/100 g dw). In-vitro, RM-B exhibited promising radical scavenging activity against 2,2′-azino-bis(3-éthylbenzothiazoline-6-sulphonique) (ABTS+·) (IC50: 247 µg/mL), hydrogen peroxide radicals (IC50: 626 µg/mL) and inhibited oxidative red blood cells hemolysis (IC50: 120 µg/mL), RM-B also showed strong reducing power (982 µM FeSO4/mg extract). In-vivo, RM-B improved the radical scavenging ability and reducing power of plasma and enhanced liver antioxidant status by increasing catalase and reduced glutathione levels and decreasing malondialdhyde levels without altering the key serum biochemical parameters reflecting liver and kidney functions. Polyphenols identified using capillary LC-DAD and LC–MS/MS analyses like hesperidin (131.2 mg/100 g dw), rutin (29.0 mg/100 g dw) and p-coumaric acid (5.8 mg/100 g dw), may be responsible for the health promoting effects of RM-B. Conclusion We may conclude that R. macrophyllus Desf. is a good source of beneficial polyphenols with strong antioxidant, anti-hemolytic and health-promoting effects, which promotes its use in pharmaceutical, medicinal and nutraceutical industries.