Person:
Rosales Conrado, Noelia

First Name

Noelia

Last Name

Rosales Conrado

URI

https://hdl.handle.net/20.500.14352/76222

Affiliation

Universidad Complutense de Madrid

Faculty / Institute

Ciencias Químicas

Department

Química Analítica

Area

Química Analítica

Identifiers

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Search Results

Now showing 1 - 10 of 27

Unravelling the in vitro and in vivo potential of selenium nanoparticles in Alzheimer’s disease: A bioanalytical review
(Talanta, 2023) Vicente Zurdo, David; Rosales Conrado, Noelia; León González, María Eugenia De
Alzheimer’s disease (AD) is a devastating neurodegenerative disorder characterized by progressive cognitive decline and the accumulation of beta-amyloid plaques and tau tangles in the brain. Current therapies have limited efficacy, prompting the search for novel treatments. Selenium nanoparticles (SeNPs) have emerged as promising candidates for AD therapy due to their unique physicochemical properties and potential therapeutic effects. This review provides an overview of SeNPs and their potential application in AD treatment, as well as the main bioanalytical techniques applied in this field. SeNPs possess antioxidant and anti-inflammatory properties, making them potential candidates to combat the oxidative stress and neuroinflammation associated with AD. Moreover, SeNPs have shown the ability to cross the blood-brain barrier (BBB), allowing them to target brain regions affected by AD pathology. Various methods for synthesizing SeNPs are explored, including chemical,physical and biological synthesis approaches. Based on the employment of algae, yeast, fungi, and plants, green methods offer a promising and biocompatible alternative for SeNPs production. In vitro studies have demonstrated the potential of SeNPs in reducing beta-amyloid aggregation and inhibiting tau hyperphosphorylation, providing evidence of their neuroprotective effects on neuronal cells. In vivo studies using transgenic mousem models and AD-induced symptoms have shown promising results, with SeNPs treatment leading to cognitive improvements and reduced amyloid plaque burden in the hippocampus. Looking ahead, future trends in SeNPs research involve developing innovative brain delivery strategies to enhance their therapeutic potential, exploring alternative animal models to complement traditional mouse studies, and investigating multi-targeted SeNPs formulations to address multiple aspects of AD pathology. Overall, SeNPs represent a promising avenue for AD treatment, and further research in this field may pave the way for effective and much-needed therapeutic interventions for individuals affected by this debilitating disease.
Valorization of prunus seed oils: fatty acids composition and oxidative stability
(Molecules, 2023) Rodríguez-Blázquez, Sandra; Gómez Mejía, Esther; Rosales Conrado, Noelia; León González, María Eugenia De; García-Sánchez, Beatriz; Miranda Carreño, Rubén
Prunus fruit seeds are one of the main types of agri-food waste generated worldwide during the processing of fruits to produce jams, juices and preserves. To valorize this by-product, the aim of this work was the nutritional analysis of peach, apricot, plum and cherry seeds using the official AOAC methods, together with the extraction and characterization of the lipid profile of seed oils using GC-FID, as well as the measurement of the antioxidant activity and oxidative stability using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging method. Chemometric tools were required for data evaluation and the obtained results indicated that the main component of seeds were oils (30–38%, w). All seed oils were rich in oleic (C18:1n9c) and linoleic (C18:2n6c) acids and presented heart-healthy lipid indexes. Oil antioxidant activity was estimated in the range IC50 = 20–35 mg·mL−1, and high oxidative stability was observed for all evaluated oils during 1–22 storage days, with the plum seed oil being the most antioxidant and stable over time. Oxidative stability was also positively correlated with oleic acid content and negatively correlated with linoleic acid content. Therefore, this research showed that the four Prunus seed oils present interesting healthy characteristics for their use and potential application in the cosmetic and nutraceutical industries.
Neuroprotective Action of Multitarget 7-Aminophenanthridin-6(5H)-one Derivatives against Metal-Induced Cell Death and Oxidative Stress in SN56 Cells
(ACS Chemical Neuroscience, 2021) Moyano-Cires Ivanoff, Paula Viviana; Vicente Zurdo, David; Blázquez-Barbadillo, Cristina; Menéndez Ramos, José Carlos; González Matilla, Juan Francisco; Rosales Conrado, Noelia; Pino Sans, Javier Del
Neurodegenerative diseases have been associated with brain metal accumulation, which produces oxidative stress (OS), matrix metalloproteinases (MMPs) induction, and neuronal cell death. Several metals have been reported to downregulate both the nuclear factor erythroid 2-related factor 2 (Nrf2) pathway and the antioxidant enzymes regulated by it, mediating OS induction and neurodegeneration. Among a recently discovered family of multitarget 7-amino-phenanthridin-6-one derivatives (APH) the most promising compounds were tested against metal-induced cell death and OS in SN56 cells. These compounds, designed to have chelating activity, are known to inhibit some MMPs and to present antioxidant and neuroprotective effects against hydrogen peroxide treatment to SN56 neuronal cells. However, the mechanisms that mediate this protective effect are not fully understood. The obtained results show that compounds APH1, APH2, APH3, APH4, and APH5 were only able to chelate iron and copper ions among all metals studied and that APH3, APH4, and APH5 were also able to chelate mercury ion. However, none of them was able to chelate zinc, cadmium, and aluminum, thus exhibiting selective chelating activity that can be partly responsible for their neuroprotective action. Otherwise, our results indicate that their antioxidant effect is mediated through induction of the Nrf2 pathway that leads to overexpression of antioxidant enzymes. Finally, these compounds exhibited neuroprotective effects, reversing partially or completely the cytotoxic effects induced by the metals studied depending on the compound used. APH4 was the most effective and safe compound.
Adding sustainability in analytical chemistry education through monitoring aquarium water quality
(Sustainable Chemistry, 2023) Rosales Conrado, Noelia; Peña Martínez, Juan
This paper introduces a captivating topic for upper-level analytical chemistry capstone projects, focusing on aquarium water analysis. This provides a more comprehensive understanding of the role of analytical chemistry towards sustainability and its environmental, economic, societal and education dimensions. Regarding the crucial role of maintaining optimal aquarium water quality for the welfare of aquatic life, students are tasked with envisioning and executing the measurement of key parameters, including pH, ammonium, nitrite, and nitrate contents. This hands-on experience not only engages students in real-world applications, but also allows them to delve into essential analytical chemistry principles. They carefully select measurement methods, considering factors such as instrument availability, ease of use, precision and sensitivity requirements, sample size, and matrix effects. Besides fostering the acquisition of technical and soft skills, one notable aspect of this type of project is the exceptionally high student satisfaction. Furthermore, the project’s outcomes have proven to be significant predictors of learning achievements. Additionally, it lays the foundation for exploring potential designs of aquaponics systems and fosters interdisciplinary projects, expanding the practical applications in the field of chemistry education. Overall, these projects exemplify enriching and engaging educational experiences that empower students with valuable skills and knowledge while encouraging them to explore novel avenues in analytical chemistry.
A combined analytical-chemometric approach for the in vitro determination of polyphenol bioaccessibility by simulated gastrointestinal digestion
(Analytical and Bioanalytical Chemistry, 2022) Gómez Mejía, Esther; Rosales Conrado, Noelia; León González, María Eugenia; Valverde de la Fuente, Alejandro; Madrid Albarrán, Yolanda
In this study, an integrated characterisation through polyphenol and cafeine content and antioxidant activity was combined with chemometric analysis to assess the efects of simulated in vitro gastrointestinal digestion on the bioaccessibility of these bioactive compounds from nine diferent tea infusions. Tea infusions were characterised based on total favonoids, total polyphenols and antioxidant activity, together with the determination of individual polyphenol content. Fourteen phenolic compounds, including phenolic acids, stilbenes and favonoids, were selected based on their reported bioactivity and high accessibility, attributed to their low molecular weight. Both polyphenols and cafeine were initially monitored in raw tea infusions and through the diferent digestion stages (salivary, gastric and duodenal) by capillary high performance liquid chromatography coupled to diode array detection (cHPLC-DAD) and/or HPLC coupled to a triple quadrupole mass analyser (HPLC–MS/MS). Multivariate analysis of the studied bioactives, using principal component analysis and cluster analysis, revealed that the decafeination process seems to increase the stability and concentration of the compounds evaluated during digestion. The greatest transformations occurred mainly in the gastric and duodenal stages, where low bioactivity indices (IVBA) were shown for resveratrol and cafeic acid (IVBA=0%). In contrast, the polyphenols gallic acid, chlorogenic acid and quercetin gave rise to their availability in white, green and oolong infusion teas (IVBA>90%). Furthermore, highly fermented black and pu-erh varieties could be designated as less bioaccessible environments in the duodenum with respect to the tested compounds.
Valorization of citrus reticulata blanco peels to produce enriched wheat bread: phenolic bioaccessibility and antioxidant potential
(ANTIOXIDANTS, 2023) Gómez Mejía, Esther; Sacristán, Iván; Rosales Conrado, Noelia; León González, María Eugenia De; Madrid Albarrán, María Yolanda
The fortification of foods with bioactive polyphenols aims to improve their functional properties and to provide health benefits. Yet, to exert their benefits, phenolic compounds must be released from the food matrix and absorbed by the small intestine after digestion, so assessing their bioaccessibility is crucial to determine their potential role. This work aims to incorporate Citrus reticulata Blanco peel extracts into wheat bread as a promising opportunity to increase their bioactive potential, along with supporting the sustainable management of citrus-industry waste. A control and a wheat bread enriched at 2% and 4% (w/v) with a phenolic extract from mandarin peels were prepared and analyzed for antioxidant activity and phenolic composition using LC-MS and UV-Vis spectrophotometry. In addition, in vitro digestion was performed, and the digested extracts were analyzed with HPLC-MS/MS. The results showed a significant increase in total flavonoid content (TFC, 2.2 ± 0.1 mg·g−1), antioxidant activity (IC50 = 37 ± 4 mg·g−1), and contents of quercetin, caffeic acid, and hesperidin in the 4% (w/v) enriched bread. Yet, most polyphenols were completely degraded after the in vitro digestion process, barring hesperidin (159 ± 36 μg·g−1), highlighting the contribution of citrus enrichment in the development of an enriched bread with antioxidant potential.
Phenolic profile, safety, antioxidant and anti-inflammatory activities of wasted Bunium ferulaceum Sm. aerial parts
(Food Research International, 2022) Deghima, Amirouche; Righi, Nadjat; Rosales Conrado, Noelia; León González, María Eugenia De; Baali, Faiza; Gómez Mejía, Esther; Madrid Albarrán, María Yolanda; Bedjou, Fatiha; ELSEVIER
The pharmaceutical and nutraceutical industries benefit greatly from recycling and transforming non-utilized parts of medicinal plants from agro-industrial operations into value added products. Hence, the aim of this work was to study the potential nutraceutical and pharmaceutical applications of Bunium ferulaceum Sm. aerial parts, in order to maximize their value. The phenolic profile of their hydromethanolic extract was determined and its antioxidant activity was evaluated in vitro and in vivo alongside with its anti-inflammatory activity and safety profile. The extract exerted an in vitro antioxidant activity mainly through radical scavenging (DPPH IC50: 14.0 ± 0.3 μg/ml) and iron chelating ability (24 ± 2 μg/ml), while, in vivo, the extract did not cause any mortality or visible signs of acute toxicity at high dose (2000 mg/kg body weight). The supplementation of the extract at different doses improved mice liver redox state by increasing catalase and reduced glutathione levels and reducing lipid peroxidation, without causing any toxicity. Moreover, the extract efficiently inhibited xylene induced ear inflammation (62 %). These different bioactivities were linked to the phenolic compounds present in the extract, particularly, chlorogenic acid (78 ± 6 mg/g extract), rutin (44 ± 2 mg/g extract) and hesperidin (56 ± 9 mg/g extract). However, further studies should be carried out on the isolated major compounds found in the extract to correlate the activity with these compounds or their mixture. The wasted aerial parts of Bunium ferulaceum Sm. proved to be a valuable source of polyphenols and exhibited interesting health promoting effects with no toxicity. Thus, Bunium ferulaceum Sm. aerial parts can be included in nutraceutical formulations or used as functional food and the extracted compounds may be used as an alternative food preservative.
Project number: 71
Diseño de un e-portfolio para la adquisición de competencias transversales en Educación para la Sostenibilidad usando las TIC
(2021) Peña Martínez, Juan; Fernández Lázaro, Gloria; Pérez López, Raquel; Ortiz Iniesta, María Jesús; Lara Caballero, Ana; Rosales Conrado, Noelia; Andrés Casado, Nuria
Diseño de un portafolio digital, incluido sistema de rúbrica, para integrar la Educación para la Sostenibilidad en la enseñanza-aprendizaje de las ciencias experimentales favoreciendo el empleo de las TIC y competencias como el razonamiento reflexivo.
Project number: PIMCD213/23-24
Seminario permanente sobre educación para la paz
(2024) Peña Martínez, Juan; Rosales Conrado, Noelia; Salcedo Megales, Damián; Gonzalo Llera, Carolina; Momobela Rosario, Felicitas Carlota; Parello, Mara Lourdes; Li, Minghui; Jia, Qian
Se ha creado un espacio universitario donde, gracias a destacados especialistas en paz, desarrollo, seguridad y educación transformadora, se han aportado enfoques y prácticas para afrontar los desafíos de la convivencia social e impulsar la cultura de la paz, todo ello muy necesario para la formación inicial de maestros y maestras. Por otro lado, se ha facilitado y experimentado una propuesta didáctica orientada a promover el dialogo y respeto mutuo en el ámbito de la Didáctica de la Química. Finalmente, se ha llevado a cabo un estudio exploratorio sobre la actitud de los futuros docentes (estudiantes de la Facultad de Educación - Centro de Formación del Profesorado de la Universidad Complutense de Madrid) hacia la paz y la guerra.
Clase invertida de espectroscopía: una experiencia en el marco de un Programa de Excelencia para Bachillerato
(Educación Química, 2020) Gómez Gómez, Beatriz; Peña Martínez, Juan; Rosales Conrado, Noelia
En el presente trabajo se describe una experiencia didáctica en un curso de Bachillerato, perteneciente al Programa de Excelencia, de un centro escolar de la Comunidad de Madrid (España). Mediante un modelo de clase invertida o flipped classroom (de su traducción en inglés), se abordaron los fundamentos relacionados con las técnicas de análisis espectroscópico y se realizaron actividades experimentales como la preparación de un espectroscopio “casero”. Tras la intervención, se llevó a cabo una encuesta de satisfacción para conocer la percepción de los estudiantes sobre las actividades realizadas y sobre el modelo de enseñanza-aprendizaje aplicado. Los alumnos han valorado positivamente la nueva metodología, si bien, su aplicación ha generado un cierto desconcierto que puede generar dudas a la hora de aplicar el enfoque de clase invertida a otras asignaturas.