Person: Benito Peña, María Elena
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First Name
María Elena
Last Name
Benito Peña
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Químicas
Department
Química Analítica
Area
Química Analítica
Identifiers
7 results
Search Results
Now showing 1 - 7 of 7
Publication Comparative Study of the Performance of Two Different Luciferases for the Analysis of Fumonisin B1 in Wheat Samples(Wiley-VCH, 2022-02-17) Luque Uría, Álvaro; Peltomaa, Riikka; Navarro Duro, Marina; Fikacek, Sabrina; Head, Trajen; Deo, Sapna K.; Daunert, Sylvia; Benito Peña, María Elena; Moreno Bondi, María CruzThe development of two different immunoassays for the determination of fumonisin B1 in wheat samples is reported. A previously described mimopeptide for fumonisin B1 (FB1) was used to produce fusion proteins in combination with two different luciferases: Gaussia luciferase (GLuc) and NanoLuc luciferase (NLuc). The production, expression and the development of two immunoassays based on these fusion proteins (A2- GLuc and A2-NLuc) is detailed. The assay showing the best performance, A2-NLuc, with a limit of detection of 0.61 ngmL 1 and a dynamic range from 1.9 to 95 ngmL 1 , was employed for the analysis of spiked wheat samples, a reference matrix material, as well as naturally contaminated wheat samples. The recoveries obtained in the spiked samples were acceptable, between 81.5 and 109%, with relative standard deviations lower than 14%. The analysis of naturally contaminated wheat was validated by a liquid chromatography coupled to tandem mass detection method.Publication Desarrollo y validación de métodos analíticos basados en nuevos elementos de reconocimiento molecular para la determinación de antibióticos -Lactámicos en muestras de interés agroalimentario y medioambiental(Universidad Complutense de Madrid, Servicio de Publicaciones, 2007) Benito Peña, María Elena; Moreno Bondi, María CruzEsta Tesis Doctoral se ha realizado en el marco de un Proyecto del V Programa Marco, subvencionado por la Unión Europea dentro del área de Calidad de Vida y Gestión de Recursos Vivos, titulado "Cartridges with molecularly imprinted recognition elements for antibiotic residues monitoring in milk": CREAM (Ref. QLK-1999-00902). La investigación realizada en el marco de la tesis presentada se puede resumir en los siguientes puntos: 1. Caracterización foto física y fotoquímica de los derivados fluorescentes de penicilinas para su aplicación en el análisis de antibióticos. 2. Desarrollo de un fluoro ensayo competitivo, basado en el empleo de mips, como elementos de reconocimiento selectivo para el análisis de antibióticos de la familia de penicilinas en alimentos y medicamentos. 3. Desarrollo de un fluoroinmunoensayo competitivo, totalmente automatizado, basado en el empleo de los derivados fluorescentes de penicilinas y anticuerpos poli clónales selectivos a antibióticos -lacta micos. Aplicación del biosensor al análisis de muestras de leche y medioambientales. 4. Desarrollo de un método cromatográfico basado en la técnica de HPLC con detección UV para la determinación de los residuos de antibióticos -lacta micos (amoxicilina, ampicilina, penicilina G, penicilina V, oxacilina, cloxacilina, nafcilina y dicloxacilina) en muestras biológicas y medioambientales con el fin de validar los dispositivos desarrollados. Este objetivo incluye la optimización de un método de extracción en fase sólida (SPE) para la preconcentración y limpieza de las muestras seleccionadas.Publication Biosensing Based on Nanoparticles for Food Allergens Detection(MDPI, 2018-04-04) Gómez Arribas, Lidia Nazaret; Benito Peña, María Elena; Hurtado Sánchez, María del Carmen; Moreno Bondi, María CruzFood allergy is one of the major health threats for sensitized individuals all over the world and, over the years, the food industry has made significant efforts and investments to offer safe foods for allergic consumers. The analysis of the concentration of food allergen residues in processing equipment, in raw materials or in the final product, provides analytical information that can be used for risk assessment as well as to ensure that food-allergic consumers get accurate and useful information to make their food choices and purchasing decisions. The development of biosensors based on nanomaterials for applications in food analysis is a challenging area of growing interest in the last years. Research in this field requires the combined efforts of experts in very different areas including food chemistry, biotechnology or materials science. However, the outcome of such collaboration can be of significant impact on the food industry as well as for consumer’s safety. These nanobiosensing devices allow the rapid, selective, sensitive, cost-effective and, in some cases, in-field, online and real-time detection of a wide range of compounds, even in complex matrices. Moreover, they can also enable the design of novel allergen detection strategies. Herein we review the main advances in the use of nanoparticles for the development of biosensors and bioassays for allergen detection, in food samples, over the past few years. Research in this area is still in its infancy in comparison, for instance, to the application of nanobiosensors for clinical analysis. However, it will be of interest for the development of new technologies that reduce the gap between laboratory research and industrial applications.Publication "Chem-game", el juego como estrategia para la dinamización del aprendizaje y la evaluación de conocimientos en Química General(2020-05-18) Moreno Bondi, María Cruz; Gutiérrez Alonso, Angel; Lainez Ferrando, Alfredo; Benito Peña, María Elena; Glahn Martínez, Ana Bettina; García Pinar, Gioele; Castellano Perdomo, Raúl; Navarro Duro, Marina; Peng, Wenrong; Morales Benítez, Noelia; Rubio Ramón, Pablo Ignacio; Olmos Alonso, InésEste proyecto pretende aplicar la "gamificación" en la enseñanza de la asignatura de Química General de primer curso del grado en Química para fomentar la formación, creatividad, compromiso y la capacidad de trabajo en equipo de los estudiantes.Publication Material docente interactivo en inglés para la enseñanza práctica y el autoaprendizaje de (bio)sensores químicos ópticos en Grado y Máster(2018-01-15) Descalzo López, Ana Belén; Orellana Moraleda, Guillermo; Moreno Bondi, María Cruz; Benito Peña, María Elena; Urraca Ruiz, JavierMaterial docente (guiones de prácticas, vídeos, cuestionarios multi-respuesta) en inglés para asignaturas de Grado y Máster relacionadas con el tema de sensores (bio)químicos ópticos y la preparación de nanomateriales aplicados en sensores ópticosPublication Improved performance of SPR sensors by a chemical etching of tapered optical fibers(Elsevier Sci. Ltd., 2011-08) Díaz Herrera, Natalia; Esteban Martínez, Óscar; Navarrete Fernández, María Cruz; González Cano, Agustín; Benito Peña, María Elena; Orellana Moraleda, GuillermoWe present the results of a chemical attack on the optical fiber surface previous to the deposition of the double layer (metal plus dielectric) in Double-layer uniform-waist tapered fibers (DLUWTs) used for the development of SPR sensors. It is shown how this simple chemical treatment increases the roughness of the surface and permits improvement of the stability of the deposits and the general performance of the sensors. The obtained devices are robust and very compact, their sensitivity is good and repeatability of the measurements is remarkably increased. The procedure can be useful for any fiber-optic sensor.Publication Optical Biosensors for Label-Free Detection of Small Molecules(MDPI, 2018-11-24) Peltomaa, Riikka Johanna; Glahn Martínez, Ana Bettina; Benito Peña, María Elena; Moreno Bondi, María CruzLabel-free optical biosensors are an intriguing option for the analyses of many analytes, as they offer several advantages such as high sensitivity, direct and real-time measurement in addition to multiplexing capabilities. However, development of label-free optical biosensors for small molecules can be challenging as most of them are not naturally chromogenic or fluorescent, and in some cases, the sensor response is related to the size of the analyte. To overcome some of the limitations associated with the analysis of biologically, pharmacologically, or environmentally relevant compounds of low molecular weight, recent advances in the field have improved the detection of these analytes using outstanding methodology, instrumentation, recognition elements, or immobilization strategies. In this review, we aim to introduce some of the latest developments in the field of label-free optical biosensors with the focus on applications with novel innovations to overcome the challenges related to small molecule detection. Optical label-free methods with different transduction schemes, including evanescent wave and optical fiber sensors, surface plasmon resonance, surface-enhanced Raman spectroscopy, and interferometry, using various biorecognition elements, such as antibodies, aptamers, enzymes, and bioinspired molecularly imprinted polymers, are reviewed.