Person: Blanco Gutiérrez, María Del Mar
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First Name
María Del Mar
Last Name
Blanco Gutiérrez
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Sanidad Animal
Area
Sanidad Animal
Identifiers
17 results
Search Results
Now showing 1 - 10 of 17
Item 16S-23S rRNA Internal Transcribed Spacer Region (ITS) Sequencing: A Potential Molecular Diagnostic Tool for Differentiating Lactococcus garvieae and Lactococcus petauri(Microorganisms, 2023) Stoppani, Nadia; Colussi, Silvia; Pastorino, Paolo; Prearo, Marino; Sciuto, Simona; Altinok, Ilhan; Çağrı Öztürk, Rafet; Ture, Mustafa; Vela Alonso, Ana Isabel; Blanco Gutiérrez, María Del Mar; Kotzamanidis, Charalampos; Bitchava, Konstantina; Malousi, Andigoni; Fariano, Lucio; Volpatti, Donatella; Acutis, Pier Luigi; Fernández-Garayzábal Fernández, José FranciscoLactococcus garvieae is the etiological agent of lactococcosis, a clinically and economically significant infectious disease affecting farmed rainbow trout. L. garvieae had been considered the only cause of lactococcosis for a long time; however, L. petauri, another species of the genus Lactococcus, has lately been linked to the same disease. The genomes and biochemical profiles of L. petauri and L. garvieae have a high degree of similarity. Traditional diagnostic tests currently available cannot distinguish between these two species. The aim of this study was to use the transcribed spacer (ITS) region between 16S rRNA and 23S rRNA as a potential useful molecular target to differentiate L. garvieae from L. petauri, saving time and money compared to genomics methods currently used as diagnostic tools for accurate discrimination between these two species. The ITS region of 82 strains was amplified and sequenced. The amplified fragments varied in size from 500 to 550 bp. Based on the sequence, seven SNPs were identified that separate L. garvieae from L. petauri. The 16S-23S rRNA ITS region has enough resolution to distinguish between closely related L. garvieae and L. petauri and it can be used as a diagnostic marker to quickly identify the pathogens in a lactococcosis outbreak.Item Utilization of lactose and presence of the phospho-β-galactosidase (lacG) gene in Lactococcus garvieae isolates from different sources(International microbiology: the official journal of the Spanish Society for Microbiology, 2010) Aguado Urda, Mónica; Blanco Gutiérrez, María Del Mar; Cutuli Simón, María Teresa; Aspiroz, Carmen; Tejedor, José L; Gibello Prieto, Alicia; Fernández-Garayzábal Fernández, José FranciscoThis study evaluates the utilization of lactose (Lac) and the presence of the phospho-β-galactosidase (lacG) gene as markers for distinguishing between fish (Lac-/lacG-) and dairy isolates (Lac+/lacG+) of Lactococcus garvieae, using a panel of L. garvieae isolates from different sources. None of the fish isolates produced acid from lactose (Lac-), however Lac-/lacG- isolates were observed in pigs, cows, birds and humans. Most of the dairy isolates (77.8%) were Lac+/lacG+, but some dairy isolates did not produce acid from this sugar. Data in the present study show that the ability to metabolize lactose and the presence of the lacG gene are heterogeneously scattered among L. garvieae isolates of different sources. Therefore, the use of these criteria as markers to differentiate between L. garvieae isolates of dairy and fish origin should be considered with caution.Item Spleen and head kidney differential gene expression patterns in trout infected with Lactococcus garvieae correlate with spleen granulomas(2019) Castro, R; Coll, J; Jouneau, L; Blanco Gutiérrez, María Del Mar; Rodríguez Bertos, Antonio Manuel; Fernández-Garayzábal Fernández, José Francisco; Gibello Prieto, AliciaLactococcus garvieae is a significant pathogen in aquaculture with a potential zoonotic risk. To begin to characterize the late immune response of trout to lactococcosis, we selected infected individuals showing clinical signs of lactococcosis. At the time lactococcosis clinical signs appeared, infection by L. garvieae induced a robust inflammatory response in the spleen of rainbow trout, which correlated with abundant granulomatous lesions. The response in kidney goes in parallel with that of spleen, and most of the gene regulations are similar in both organs. A correlation existed between the early inflammatory granulomas in spleen (containing macrophages with internalized L. garvieae) and up-regulated gene sets, which defined the presence of macrophages and neutrophils. This is the first analysis of the immune transcriptome of rainbow trout following L. garvieae infection during the initiation of adaptive immune mechanisms and shows a ranscriptome induction of antibody response by both IgM (+) and IgT (+) spleen B cells to respond to systemic infection. These results increase our understanding of lactococcosis and pave the way for future research to improve control measures of lactococcosis on fish farms.- Project number: 25
Item Epidemia vir(t)ual: el juego sobre virus para teléfonos inteligentes(2015) Gómez-Lucía Duato, María Esperanza; Benítez Rico, Laura; Blanco Gutiérrez, María Del Mar; Cutuli Simón, María Teresa; Domenech Gómez, Ana María; Flores Pedauyé, Ricardo; Quer Sivila, Josep; Romero Cano, JavierInforme del PIMCD 25 del año 2014. Item Alternative strategy for visceral leishmaniosis control: HisAK70-Salmonella Choleraesuis-pulsed dendritic cells(Comparative Immunology, Microbiology and Infectious Diseases, 2017) Carrión Herrero, Francisco Javier; Domínguez Bernal, Gustavo Ramón; Martínez Rodrigo, Abel; Mas Zubiri, Alicia; Blanco Gutiérrez, María Del Mar; Orden Gutiérrez, José Antonio; Fuente López, Ricardo De LaHere, we describe a novel approach that exploits an attenuated mutant of Salmonella enterica serovar Choleraesuis as carrier to deliver a plasmid encoding protein HisAK70. Subsequently, dendritic cells (DCs) were pulsed with this vaccine vector. The aim of this study was to evaluate the effectiveness of the prepared HisAK70-S. Choleraesuis-pulsed DCs (HisAK70-SAL DCs) against visceral leishmaniosis (VL). In our ex vivo model of infection, the prepared formulations could decrease parasite growth by up to 80% by augmenting the production of IL-12p40 and by reducing arginase activity (ARG). Also, BALB/c mice when immunised with this formulation showed significant reduction in parasite burden in both spleen (20% of reduction) and liver (75% of reduction). The balance of the immune ratios IFN-γ/IL-10, TNF-α/IL-10, and IgG2a/IgG1 reflected the acquisition of an improved resistant phenotype in HisAK70-SAL DCs vaccinated mice compared to control mice. Our results suggest that HisAK70-SAL DCs could be a promising alternative approach for vaccine delivery that has the potential to fight Leishmania infantum (L. infantum) infection.Item Global transcriptome analysis of Lactococcus garvieae strains in response to temperature(PloS one, 2013) Aguado Urda, Mónica; Gibello Prieto, Alicia; Blanco Gutiérrez, María Del Mar; López Alonso, Victoria; López Campos, Guillermo H; Fernández-Garayzábal Fernández, José FranciscoLactococcus garvieae is an important fish and an opportunistic human pathogen. The genomic sequences of several L. garvieae strains have been recently published, opening the possibility of global studies on the biology of this pathogen. In this study, a whole genome DNA microarray of two strains of L. garvieae was designed and validated. This DNA microarray was used to investigate the effects of growth temperature (18°C and 37°C) on the transcriptome of two clinical strains of L. garvieae that were isolated from fish (Lg8831) and from a human case of septicemia (Lg21881). The transcriptome profiles evidenced a strain-specific response to temperature, which was more evident at 18°C. Among the most significant findings, Lg8831 was found to up-regulate at 18°C several genes encoding different cold-shock and cold-induced proteins involved in an efficient adaptive response of this strain to low-temperature conditions. Another relevant result was the description, for the first time, of respiratory metabolism in L. garvieae, whose gene expression regulation was temperature-dependent in Lg21881. This study provides new insights about how environmental factors such as temperature can affect L. garvieae gene expression. These data could improve our understanding of the regulatory networks and adaptive biology of this important pathogen.Item The association of Lactococcus petauri with lactococcosis is older than expected(Aquaculture, 2023) Vela Alonso, Ana Isabel; Blanco Gutiérrez, María Del Mar; Colussi, Silvia; Charalampos, Kotzamanidis; Prearo, Marino; Altinok, Ilhan; Luigi Acutis, Pier; Volpatti, Donatella; Alba, Patricia; Feltrin, Fabiola; Ianzano, Angela; Domínguez Rodríguez, Lucas José; Fernández-Garayzábal Fernández, José FranciscoLactococcosis is a globally prevalent infectious disease that has a significant economic and sanitary impact on the rainbow trout industry. Lactococcus garvieae has traditionally been considered the only species implicated in the etiology of this disease, but Lactococcus petauri, a new species, has recently been implicated as another etiological agent. Both species cannot be distinguished by routine methods commonly used in diagnostic laboratories, resulting in their misidentification. In the present study, the identification of 48 isolates initially identified as L. garvieae was studied by determining their in-silico DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values using pairwise comparisons of their whole genome sequences and the genomes of the type strains of L. garvieae and L. petauri. The genome sequences of 37 isolates from countries in which lactococcosis can be considered endemic (Spain, Italy, Türkiye, and Greece) were obtained in this study, and the genomes of 11 isolates were retrieved from the GenBank database. Isolates from Italy, Singapore, Japan, South Korea, India, one Turkish isolate from 2013 and two Spanish isolates recovered in 1992 and 1996 were confirmed as L. garvieae. The remaining isolates from Spain and Türkiye, as well as those from Portugal, Israel, USA, and Greece were identified as L. petauri. Overall, 60.4% of isolates previously identified as L. garvieae were found to be L. petauri. These results confirm the implication of both species in the etiology of lactococcosis and suggest that L. petauri plays a significant role in the epidemiology of this disease. Some of the isolates identified as L. petauri in the present study were isolated three decades ago, indicating that its association with lactococcosis is older than might be expected from the recent descriptions. The commercial Rapid ID32 Strep system was unable to discriminate between L. garvieae and L. petauri. However, both species exhibited some biochemical differences that might serve as phenotypic markers for their presumptive recognition. Consequently, isolates that hydrolyze hippurate and produce acid from sucrose and tagatose could be presumptively recognized as L. petauri, while those that fail these tests could be identified as L. garvieae. The results of this work indicate that great attention should be given to L. petauri in the epidemiology of lactococcosis.- Project number: nº 136
Item La innovación educativa aplicada a la Ictiopatología(2018) Vela Alonso, Ana Isabel; Blanco Gutiérrez, María Del Mar; Gibello Prieto, Alicia; Cutuli Simón, María Teresa; Sánchez-Fortún Rodríguez, Sebastián; Ruiz Zarzuela, Imanol; Blas Giral, Ignacio de; Muniesa del Campo, Ana; Fernández-Garayzábal Fernández, José FranciscoSe ha elaborado un material aplicable a la docencia y que puede favorecer el ejercicio profesional de un veterinario y de otras áreas dedicados a la Acuicultura. Además, tiene el valor añadido de ser una colaboración entre docentes pertenecientes a dos Universidades. Item Inmunotrivial: un juego de autoevaluación para el aprendizaje de la inmunología(V Jornada Campus Virtual UCM: buenas prácticas e indicios de calidad, 2009) Blanco Gutiérrez, María Del Mar; Cutuli Simón, María Teresa; Domenech Gómez, Ana María; Domínguez Bernal, Gustavo Ramón; Gibello Prieto, Alicia; Gómez-Lucía Duato, María EsperanzaHoy en día la Inmunología es un instrumento fundamental de la medicina moderna, ya que posibilita la comprensión de los mecanismos de acción de los agentes patógenos y de la respuesta de defensa del organismo frente a ellos. Por tanto, el conocimiento de la Inmunología es vital para los profesionales de distintos ámbitos relacionados con Ciencias de la Salud: médicos, odontólogos, farmacéuticos, biólogos, veterinarios. Por otra parte, el proceso de adaptación curricular al EEES implica una tendencia al aprendizaje de forma más autónoma y al empleo de las nuevas tecnologías a nuestro alcance. Por estos motivos, y con la experiencia que nos proporcionan nuestras actividades tanto de investigación como docentes en Inmunología, así como la experiencia adquirida en la elaboración de nuevos materiales docentes, como el «Manual de Inmunología Veterinaria» (2007, Pearson/Prentice Hall), y el CD «Microbiología Veterinaria. Laboratorio Virtual» (2007, UCM/Editorial Complutense), nos hemos planteado un nuevo reto: el diseño de material de autoevaluación en Inmunología. Se trata de una aplicación informática con formato de juego, en el que el estudiante pone a prueba sus conocimientos en Inmunología de una manera divertida, estimulando el autoaprendizaje y la autoevaluación.Item Laboratorio virtual de microbiología veterinaria(III jornada Campus Virtual UCM : Innovación en el Campus Virtual metodologías y herramientas, 2007) Gómez-Lucía Duato, María Esperanza; Gibello Prieto, Alicia; Cutuli Simón, María Teresa; Aranaz Martín, Alicia; Blanco Gutiérrez, María Del Mar; Blanco Cancelo, José Luis; García Sánchez, Marta Eulalia; González Zorn, Bruno; Fernández-Garayzábal Fernández, José Francisco; Fernández-Valmayor Crespo, Alfredo; Fernández-Pampillón Cesteros, Ana María; Merino Granizo, JorgePara el Proyecto de Innovación Educativa se ha realizado un CD como complemento a las prácticas de la Licenciatura de Veterinaria que tienen un componente microbiológico. Incluye aspectos fundamentales del ejercicio profesional (Toma de Muestras, Bioseguridad, Laboratorio de Virología), pero que con las infraestructuras actuales es casi imposible presentarlos al alumno de forma eficaz y personalizada. Para su desarrollo hemos elaborado el material escrito, apoyándolo con imágenes y videos explicativos. La confección del CD en sí ha sido realizada por una empresa de informática, siguiendo nuestras especificaciones y requerimientos.