Person: Giné Domínguez, Elena
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First Name
Elena
Last Name
Giné Domínguez
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Medicina
Department
Biología Celular
Area
Biología Celular
Identifiers
6 results
Search Results
Now showing 1 - 6 of 6
- Project number: 374
Item I Olimpiadas de Histología UCM. Aplicación del aprendizaje basado en juegos en el conocimiento de la Histología en el grado en Medicina(2023) Martínez Mora, Mª del Carmen; Cuesta Rubio, Natalia; Sanz Miguel, Mª del Carmen; Morales García, José Ángel; Giné Domínguez, Elena; Gómez del Moral Martín Consuegra, Manuel; Cortegano Jimeno, María Isabel; López Moreno, José Antonio; Buhler, Kora Mareen Katharina; López Gómez, Ana; Jiménez Canales, Francisco Javier; Calleja Conde, Javier; Echeverry Alzate, Víctor Alfonso; Grijota Martínez, Mª CarmenEl presente proyecto ha desarrollado una actividad de aprendizaje basado en juegos (Olimpiada de Histología) para asentar los contenidos de Histología Humana en el grado en Medicina, mediante una experiencia de aprendizaje activo divertida y motivadora. Item Gut microbiota and voluntary alcohol consumption(Translational Psychiatry, 2022) Segovia Rodríguez, Lucía; Echeverry Alzate, Víctor; Rincón Pérez. Irene; Calleja Conde, Javier; Buhler, Kora Mareen Katharina; Giné Domínguez, Elena; Albert, J.; Hinojosa Poveda, José Antonio; Huertas Rodríguez, Evelio; Gómez Gallego, Felix; Bressa, C.; Rodríguez De Fonseca, Fernando Antonio; López Moreno, José Antoniolcohol is part of the usual diet of millions of individuals worldwide. However, not all individuals who drink alcohol experience the same effects, nor will everyone develop an alcohol use disorder. Here we propose that the intestinal microbiota (IMB) helps explain the different consumption patterns of alcohol among individuals. 507 humans participated in this study and alcohol consumption and IMB composition were analyzed. On the other hand, in 80 adult male Wistar rats, behavioral tests, alcohol intoxication, fecal transplantation, administration of antibiotics and collection of fecal samples were performed. For identification and relative quantification of bacterial taxa was used the bacterial 16 S ribosomal RNA gene. In humans, we found that heavy episodic drinking is associated with a specific stool type phenotype (type 1, according to Bristol Stool Scale; p < 0.05) and with an increase in the abundance of Actinobacteria (p < 0.05). Next, using rats, we demonstrate that the transfer of IMB from alcohol-intoxicated animals causes an increase in voluntary alcohol consumption in transplant-recipient animals (p < 0.001). The relative quantification data indicate that the genus Porphyromonas could be associated with the effect on voluntary alcohol consumption. We also show that gut microbiota depletion by antibiotics administration causes a reduction in alcohol consumption (p < 0.001) and altered the relative abundance of relevant phyla such as Firmicutes, Bacteroidetes or Cyanobacteria (p < 0.05), among others. Benjamini-Hochberg false discovery rate (FDR) correction was performed for multiple comparisons. These studies reveal some of the consequences of alcohol on the IMB and provide evidence that manipulation of IMB may alter voluntary alcohol consumption.Item Red Bull® energy drink increases consumption of higher concentrations of alcohol(Addiction Biology, 2018) Roldán, Marta; Echeverry-Alzate, Victor; Buhler, Kora Mareen Katharina; Sánchez-Diez, Israel J; Calleja Conde, Javier; Olmos, Pedro; Boehm, Stephen L; Maldonado, Rafael; Rodríguez De Fonseca, Fernando Antonio; Santiago, Catalina; Gómez Gallego, Felix; Giné Domínguez, Elena; López Moreno, José AntonioMixing alcohol with caffeinated energy drinks is a common practice, especially among young people. In humans, the research on this issue has mainly focused on the use of the mass-marketed energy drinks themselves, whereas in animal models, it has focused on the individual effects of their active ingredients (i.e. caffeine). Here, we have characterized how Red Bull®, one of the most consumed caffeinated energy drink worldwide, modulates operant alcohol self-administration in Wistar rats. We found that animals readily and steadily responded for Red Bull (mean: 90 responses, 30 minutes and fixed-ratio 1), which was accompanied by locomotor stimulating effects (26 percent increase). The higher the concentration of alcohol (3–20 percent), the higher the consumption of alcohol (g/kg) and associated blood alcohol levels (91.76 percent) in the mixed Red Bull–alcohol group (60 percent increase). Blood caffeine levels in the Red Bull group were 4.69 μg/ml and 1.31 μg/ml in the Red Bull–alcohol group after the 30-minute session. Because Red Bull also contains 11 percent sucrose, we examined the time course of blood glucose as well as insulin and corticosterone. The correlation between intake of Red Bull and blood glucose levels was higher at 90 minutes than 5 minutes after its consumption, and there was no relationship with blood insulin or blood corticosterone levels. Red Bull did not alter extinction and reacquisition of responding for alcohol nor did it affect relapse-like drinking. Overall, our results suggest that Red Bull might be a vulnerability factor to develop alcoholism given that it intensifies the consumption of higher concentrations of alcohol.Item Adult-onset hypothyroidism increases ethanol consumption(Psychopharmacology, 2019) Echeverry-Alzate, Victor; Buhler, Kora Mareen Katharina; Calleja Conde, Javier; Huertas Rodríguez, Evelio; Maldonado, Rafael; Rodríguez De Fonseca, Fernando Antonio; Santiago, Catalina; Gómez-Gallego, Santiago; Santos Montes, Gregorio Ángel; Giné Domínguez, Elena; López Moreno, José AntonioRationale Only in Europe it can be estimated that more than 20 million of people would be affected by hypothyroidism in some moment of their life. Given that ethanol consumption is so frequent, it would be reasonable to ask what the consequences of ethanol consumption in those individuals affected by hypothyroidism are. Objectives To study the interaction between hypothyroidism and ethanol consumption. Methods We study ethanol consumption in a rat model of methyl-mercaptoimidazole-induced-adult-onset hypothyroidism and thyroid T4/T3 hormone supplementation. Also, we studied the effects of ethanol on motor activity, memory, and anxiety. Results We found that hypothyroidism increased the voluntary ethanol consumption and that this was enhanced by thyroid hormone supplementation. Hypothyroidism was associated with motor hyperactivity which was prevented either by T4/T3 supplementation or ethanol. The relationship between hypothyroidism, ethanol, and anxiety was more complex. In an anxiogenic context, hypothyroidism and T4/T3 supplementation would increase immobility, an anxiety-like behavior, while in a less anxiogenic context would decrease rearing, a behavior related to anxiety. Regarding memory, acute ethanol administration did not alter episodic-like memory in hypothyroid rats. Gene expression of enzymes involved in the metabolism of ethanol, i.e., Adh1 and Aldh2, were altered by hypothyroidism and T4/T3 supplementation. Conclusions Our results suggest that hypothyroid patients would need personalized attention in terms of ethanol consumption. In addition, they point that it would be useful to embrace the thyroid axis in the study of ethanol addiction, including as a possible therapeutic target for the treatment of alcoholism and its comorbid disordersItem The Immune System through the Lens of Alcohol Intake and Gut Microbiota(International Journal of Molecular Sciences, 2021) Calleja Conde, Javier; Echeverry Alzate, Víctor; Buhler, Kora Mareen Katharina; Durán González, Pedro; Morales García, José Ángel; Segovia Rodríguez, Lucía; Rodríguez De Fonseca, Fernando Antonio; Giné Domínguez, Elena; López Moreno, José AntonioThe human gut is the largest organ with immune function in our body, responsible for regulating the homeostasis of the intestinal barrier. A diverse, complex and dynamic population of microorganisms, called microbiota, which exert a significant impact on the host during homeostasis and disease, supports this role. In fact, intestinal bacteria maintain immune and metabolic homeostasis, protecting our organism against pathogens. The development of numerous inflammatory disorders and infections has been linked to altered gut bacterial composition or dysbiosis. Multiple factors contribute to the establishment of the human gut microbiota. For instance, diet is considered as one of the many drivers in shaping the gut microbiota across the lifetime. By contrast, alcohol is one of the many factors that disrupt the proper functioning of the gut, leading to a disruption of the intestinal barrier integrity that increases the permeability of the mucosa, with the final result of a disrupted mucosal immunity. This damage to the permeability of the intestinal membrane allows bacteria and their components to enter the blood tissue, reaching other organs such as the liver or the brain. Although chronic heavy drinking has harmful effects on the immune system cells at the systemic level, this review focuses on the effect produced on gut, brain and liver, because of their significance in the link between alcohol consumption, gut microbiota and the immune system.Item Nalmefene is effective at reducing alcohol seeking, treating alcohol-cocaine interactions and reducing alcohol-induced histone deacetylases gene expression in blood(British Journal of Pharmacology, 2016) Calleja Conde, Javier; Echeverry Alzate, Víctor; Giné Domínguez, Elena; Buhler, Kora Mareen Katharina; Nadal, Roser; Maldonado, Rafael; Rodríguez De Fonseca, Fernando Antonio; Gual, Antoni; López Moreno, José AntonioBackground and Purpose The opioid antagonist nalmefene (selincro®) was approved for alcohol-related disorders by the European Medicines Agency in 2013. However, there have been no studies regarding the effectiveness of nalmefene when alcohol is used in combination with cocaine. Experimental Approach Using operant alcohol self-administration in Wistar rats and qRT-PCR, we evaluated (i) the dose–response curve for s.c. and p.o. nalmefene; (ii) the effects of nalmefene with increasing concentrations of alcohol; (iii) the efficacy of nalmefene on cocaine-potentiated alcohol responding; and (iv) the gene expression profiles of histone deacetylases (Hdac1–11) in peripheral blood in vivo and in the prefrontal cortex, heart, liver and kidney post mortem. Key Results S.c. (0.01, 0.05, 0.1 mg·kg−1) and p.o. (10, 20, 40 mg·kg−1) nalmefene dose-dependently reduced alcohol-reinforced responding by up to 50.3%. This effect of nalmefene was not dependent on alcohol concentration (10, 15, 20%). Cocaine potentiated alcohol responding by approximately 40% and nalmefene (0.05 mg·kg−1) reversed this effect of cocaine. Alcohol increased Hdac gene expression in blood and nalmefene prevented the increases in Hdacs 3, 8, 5, 7, 9, 6 and 10. In the other tissues, alcohol and nalmefene either did not alter the gene expression of Hdacs, as in the prefrontal cortex, or a tissue-Hdac-specific effect was observed. Conclusions and Implications Nalmefene might be effective as a treatment for alcohol-dependent patients who also use cocaine. Also, the expression of Hdacs in peripheral blood might be useful as a biomarker of alcohol use and drug response.