Person: Giné Domínguez, Elena
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First Name
Elena
Last Name
Giné Domínguez
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Medicina
Department
Biología Celular
Area
Biología Celular
Identifiers
19 results
Search Results
Now showing 1 - 10 of 19
Item Classic psychedelics and alcohol use disorders: A systematic review of human and animal studies(Addiction Biology, 2022) Calleja Conde, Javier; Morales García, Jose Angel; Echeverry Alzate, Víctor; Bühler, Kora Mareen; Giné Domínguez, Elena; López Moreno, José AntonioClassic psychedelics refer to substances such as lysergic acid diethylamide (LSD), psilocybin, ayahuasca, and mescaline, which induce altered states of consciousness by acting mainly on 5-HT2A receptors. Recently, the interest of psychedelics as pharmacological treatment for psychiatric disorders has increased significantly, including their use on problematic use of alcohol. This systematic review is aimed to analyse the last two decades of studies examining the relationship between classic psychedelics and alcohol consumption. We searched PubMed and PsycInfo for human and preclinical studies published between January 2000 to December 2021. The search identified 639 publications. After selection, 27 studies were included. Human studies (n = 20) generally show promising data and seem to indicate that classic psychedelics could help reduce alcohol consumption. Nevertheless, some of these studies present methodological concerns such as low number of participants, lack of control group or difficulty in determining the effect of classic psychedelics in isolation. On the other hand, preclinical studies (n = 7) investigating the effect of these compounds on voluntary alcohol consumption are scarce and show some conflicting data. Among these compounds, psilocybin seems to show the most consistent data indicating that this compound could be a potential candidate to treat alcohol use disorders. In the absence of understanding the biological and/or psychological mechanisms, more studies including methodological quality parameters are needed to finally determine the effects of classic psychedelics on alcohol consumption.- Project number: 384
Item Competencias en comunicación médica: un arma para desmontar bulos en neurociencia(2021) Morales García, Jose Angel; Blázquez Díez, Cristina; Crespo Rivas, Gonzalo; González García, David; Puente Sainz, Marta; Jiménez Canales, Francisco Javier; Cortegano Jimeno, Maria Isabel; Giné Domínguez, Elena; Gómez del Moral Martín-Consuegra, Manuel María; Hurtado Carneiro, Verónica; López Moreno, José Antonio; Martínez Mora, María del Carmen; Pérez Martínez, David Andrés; Sanz Miguel, María del CarmenEste proyecto se diseñó con el objetivo general de favorecer la mejora en las capacidades comunicativas de los alumnos del grado en Medicina. De esta manera fomentamos que, en su futura actividad profesional, sean capaces de transmitir conocimientos y expresar argumentos de una manera clara y sencilla, pero con rigor, sabiéndose adaptar a las características concretas de cada situación y sobre todo del tipo de audiencia. Conseguimos así, una mejora en la comunicación científico-médica con compañeros de profesión, pero también en la comunicación con pacientes y sus familiares, usando un lenguaje más coloquial. En este contexto de comunicación, quisimos además proporcionar la información y herramientas necesarias para que nuestros alumnos puedan ir desarrollando habilidades que les permitan luchar contra los bulos en cuestiones de salud y más específicamente dentro del campo de la neurociencia. De esta manera, además de fomentar sus competencias transversales en comunicación, luchamos contra la neurofobia, puesto que incentivamos la pérdida del miedo a la terminología específica de las disciplinas relacionadas con el sistema nervioso, fomentando su dotes y habilidades comunicativas para expresarse también en términos divulgativos. - Project number: 374
Item I Olimpiadas de Histología UCM. Aplicación del aprendizaje basado en juegos en el conocimiento de la Histología en el grado en Medicina(2023) Martínez Mora, Mª del Carmen; Cuesta Rubio, Natalia; Sanz Miguel, Mª del Carmen; Morales García, José Ángel; Giné Domínguez, Elena; Gómez del Moral Martín Consuegra, Manuel; Cortegano Jimeno, María Isabel; López Moreno, José Antonio; Buhler, Kora Mareen Katharina; López Gómez, Ana; Jiménez Canales, Francisco Javier; Calleja Conde, Javier; Echeverry Alzate, Víctor Alfonso; Grijota Martínez, Mª CarmenEl presente proyecto ha desarrollado una actividad de aprendizaje basado en juegos (Olimpiada de Histología) para asentar los contenidos de Histología Humana en el grado en Medicina, mediante una experiencia de aprendizaje activo divertida y motivadora. Item Gut microbiota and voluntary alcohol consumption(Translational Psychiatry, 2022) Segovia Rodríguez, Lucía; Echeverry Alzate, Víctor; Rincón Pérez. Irene; Calleja Conde, Javier; Buhler, Kora Mareen Katharina; Giné Domínguez, Elena; Albert, J.; Hinojosa Poveda, José Antonio; Huertas Rodríguez, Evelio; Gómez Gallego, Felix; Bressa, C.; Rodríguez De Fonseca, Fernando Antonio; López Moreno, José Antoniolcohol is part of the usual diet of millions of individuals worldwide. However, not all individuals who drink alcohol experience the same effects, nor will everyone develop an alcohol use disorder. Here we propose that the intestinal microbiota (IMB) helps explain the different consumption patterns of alcohol among individuals. 507 humans participated in this study and alcohol consumption and IMB composition were analyzed. On the other hand, in 80 adult male Wistar rats, behavioral tests, alcohol intoxication, fecal transplantation, administration of antibiotics and collection of fecal samples were performed. For identification and relative quantification of bacterial taxa was used the bacterial 16 S ribosomal RNA gene. In humans, we found that heavy episodic drinking is associated with a specific stool type phenotype (type 1, according to Bristol Stool Scale; p < 0.05) and with an increase in the abundance of Actinobacteria (p < 0.05). Next, using rats, we demonstrate that the transfer of IMB from alcohol-intoxicated animals causes an increase in voluntary alcohol consumption in transplant-recipient animals (p < 0.001). The relative quantification data indicate that the genus Porphyromonas could be associated with the effect on voluntary alcohol consumption. We also show that gut microbiota depletion by antibiotics administration causes a reduction in alcohol consumption (p < 0.001) and altered the relative abundance of relevant phyla such as Firmicutes, Bacteroidetes or Cyanobacteria (p < 0.05), among others. Benjamini-Hochberg false discovery rate (FDR) correction was performed for multiple comparisons. These studies reveal some of the consequences of alcohol on the IMB and provide evidence that manipulation of IMB may alter voluntary alcohol consumption.Item C/EBP regulates TFAM expression, mitochondrial function and autophagy in cellular models of Parkinson’s Disease(International Journal of Molecular Sciences, 2023) Sierra Magro, Ana; Giné Domínguez, Elena; Morales García, José Ángel; Bartolome, Fernando; Lozano Muñoz, David; Alarcón Gil, Jesús; Sanz Sancristobal, Marina; Alonso Gil, Sandra; Cortes Canteli, Marta; Carro, Eva; Perez Castillo, AnaLa enfermedad de Parkinson (EP) es un trastorno neurodegenerativo que resulta de la degeneración de las neuronas dopaminérgicas de la sustancia negra pars compacta (SNpc). Dado que sólo se dispone de tratamientos sintomáticos, se necesitan nuevas dianas celulares y moleculares implicadas en el inicio y la progresión de esta enfermedad para desarrollar tratamientos eficaces. Los niveles del factor de transcripción CCAAT/Enhancer Binding Protein β (C/EBPβ) están alterados en pacientes con diversas enfermedades neurodegenerativas, lo que sugiere que puede ser una buena diana terapéutica para el tratamiento de la EP. Mediante la combinación de datos genéticos in silico se generó una lista de genes implicados en la EP que pueden ser regulados por C/EBPβ, entre los que se encuentra el factor de transcripción mitocondrial A (TFAM). En este trabajo, observamos que la sobreexpresión de C/EBPβ aumentaba la actividad promotora de TFAM. Sin embargo, la regulación a la baja de C/EBPβ en diferentes modelos celulares de EP/neuroinflamación produjo un aumento de los niveles de TFAM, junto con otros marcadores mitocondriales. Esto nos llevó a proponer una acumulación de mitocondrias no funcionales posiblemente debido a la alteración de su degradación autofágica en ausencia de C/EBPβ. Entonces, concluimos que C/EBPβ no sólo está involucrado en procesos dañinos que ocurren en la EP, como la inflamación, sino que también está implicado en la función mitocondrial y la autofagia en condiciones similares a la EP.- Project number: PIMCD207
Item Desarrollo e implementación de una herramienta de evaluación en los tribunales de los Trabajos de Fin de Grado y Fin de Máster en las ciencias de la salud(2016) Sanz Miguel, María del Carmen; Giné Domínguez, Elena; Navas Hernández, Ángeles; Hurtado Carneiro, Verónica; Benito Miguel, Marta; Gutiérrez Nogués, Ángel; Dongil Sánchez, Pilar; Pérez García, Ana Item Red Bull® energy drink increases consumption of higher concentrations of alcohol(Addiction Biology, 2018) Roldán, Marta; Echeverry-Alzate, Victor; Buhler, Kora Mareen Katharina; Sánchez-Diez, Israel J; Calleja Conde, Javier; Olmos, Pedro; Boehm, Stephen L; Maldonado, Rafael; Rodríguez De Fonseca, Fernando Antonio; Santiago, Catalina; Gómez Gallego, Felix; Giné Domínguez, Elena; López Moreno, José AntonioMixing alcohol with caffeinated energy drinks is a common practice, especially among young people. In humans, the research on this issue has mainly focused on the use of the mass-marketed energy drinks themselves, whereas in animal models, it has focused on the individual effects of their active ingredients (i.e. caffeine). Here, we have characterized how Red Bull®, one of the most consumed caffeinated energy drink worldwide, modulates operant alcohol self-administration in Wistar rats. We found that animals readily and steadily responded for Red Bull (mean: 90 responses, 30 minutes and fixed-ratio 1), which was accompanied by locomotor stimulating effects (26 percent increase). The higher the concentration of alcohol (3–20 percent), the higher the consumption of alcohol (g/kg) and associated blood alcohol levels (91.76 percent) in the mixed Red Bull–alcohol group (60 percent increase). Blood caffeine levels in the Red Bull group were 4.69 μg/ml and 1.31 μg/ml in the Red Bull–alcohol group after the 30-minute session. Because Red Bull also contains 11 percent sucrose, we examined the time course of blood glucose as well as insulin and corticosterone. The correlation between intake of Red Bull and blood glucose levels was higher at 90 minutes than 5 minutes after its consumption, and there was no relationship with blood insulin or blood corticosterone levels. Red Bull did not alter extinction and reacquisition of responding for alcohol nor did it affect relapse-like drinking. Overall, our results suggest that Red Bull might be a vulnerability factor to develop alcoholism given that it intensifies the consumption of higher concentrations of alcohol.- Project number: 168
Item La figura de Cajal: Vacuna contra la neurofobia y estímulo de vocaciones investigadoras para los estudiantes de medicina(2019) Sanz Miguel, María del Carmen; Martínez Mora, María del Carmen; Giné Domínguez, Elena; Morales García, José Angel; López Moreno, José Antonio; Zemanova, Marketa; Pérez Martínez, David A.; Jiménez Canales, Francisco JavierProponemos actividades centradas en D. Santiago Ramón y Cajal dirigidas a prevenir la neurofobia (miedo a las neurociencias y a la neurología clínica) entre los estudiantes de Medicina y fomentar su interés por la investigación biomédica traslacional. Item Long-term effects of intermittent adolescent alcohol exposure in male and female rats(Frontiers in Behavioral Neuroscience, 2017) Marco López, Eva María; Peñasco, Sara; Hernández, María Donina; Gil, Anabel; Borcel, Erika; Moya, Marta; Giné Domínguez, Elena; López Moreno, José Antonio; Guerri, Consuelo; López Gallardo, Meritxell; Rodríguez de Fonseca, FernandoAlcohol is a serious public health concern that has a differential impact on individuals depending upon age and sex. Patterns of alcohol consumption have recently changed: heavy episodic drinking—known as binge-drinking—has become most popular among the youth. Herein, we aimed to investigate the consequences of intermittent adolescent alcohol consumption in male and female animals. Thus, Wistar rats were given free access to ethanol (20% in drinking water) or tap water for 2-h sessions during 3 days, and for an additional 4-h session on the 4th day; every week during adolescence, from postnatal day (pnd) 28–52. During this period, animals consumed a moderate amount of alcohol despite blood ethanol concentration (BEC) did not achieve binge-drinking levels. No withdrawal signs were observed: no changes were observed regarding anxiety-like responses in the elevated plus-maze or plasma corticosterone levels (pnd 53–54). In the novel object recognition (NOR) test (pnd 63), a significant deficit in recognition memory was observed in both male and female rats. Western Blot analyses resulted in an increase in the expression of synaptophysin in the frontal cortex (FC) of male and female animals, together with a decrease in the expression of the CB2R in the same brain region. In addition, adolescent alcohol induced, exclusively among females, a decrease in several markers of dopaminergic and serotonergic neurotransmission, in which epigenetic mechanisms, i.e., histone acetylation, might be involved. Taken together, further research is still needed to specifically correlate sex-specific brain and behavioral consequences of adolescent alcohol exposure.- Project number: 101
Item Santiago Ramón y Cajal: un modelo de excelencia para desarrollar competencias en el Grado en Medicina(2018) Martínez Mora, María del Carmen; Giné Domínguez, Elena; Sanz Miguel, María del Carmen; Varas Fajardo, Alberto; Pérez Martínez, David Andrés; Valiño Seoane, Iria; Martínez Murillo, Ricardo; De Castro Soubriet, FernandoEl presente proyecto ha acercado la figura de Santiago Ramón y Cajal a los estudiantes de Medicina como estrategia para el desarrollo de competencias generales, específicas y transversales necesarias para la formación de profesionales competentes. Se han realizado una serie de actividades en torno a nuestro Premio Nobel que han involucrado a docentes, investigadores y clínicos.