Person: Giné Domínguez, Elena
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First Name
Elena
Last Name
Giné Domínguez
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Medicina
Department
Biología Celular
Area
Biología Celular
Identifiers
9 results
Search Results
Now showing 1 - 9 of 9
Item Classic psychedelics and alcohol use disorders: A systematic review of human and animal studies(Addiction Biology, 2022) Calleja Conde, Javier; Morales García, Jose Angel; Echeverry Alzate, Víctor; Bühler, Kora Mareen; Giné Domínguez, Elena; López Moreno, José AntonioClassic psychedelics refer to substances such as lysergic acid diethylamide (LSD), psilocybin, ayahuasca, and mescaline, which induce altered states of consciousness by acting mainly on 5-HT2A receptors. Recently, the interest of psychedelics as pharmacological treatment for psychiatric disorders has increased significantly, including their use on problematic use of alcohol. This systematic review is aimed to analyse the last two decades of studies examining the relationship between classic psychedelics and alcohol consumption. We searched PubMed and PsycInfo for human and preclinical studies published between January 2000 to December 2021. The search identified 639 publications. After selection, 27 studies were included. Human studies (n = 20) generally show promising data and seem to indicate that classic psychedelics could help reduce alcohol consumption. Nevertheless, some of these studies present methodological concerns such as low number of participants, lack of control group or difficulty in determining the effect of classic psychedelics in isolation. On the other hand, preclinical studies (n = 7) investigating the effect of these compounds on voluntary alcohol consumption are scarce and show some conflicting data. Among these compounds, psilocybin seems to show the most consistent data indicating that this compound could be a potential candidate to treat alcohol use disorders. In the absence of understanding the biological and/or psychological mechanisms, more studies including methodological quality parameters are needed to finally determine the effects of classic psychedelics on alcohol consumption.- Project number: 374
Item I Olimpiadas de Histología UCM. Aplicación del aprendizaje basado en juegos en el conocimiento de la Histología en el grado en Medicina(2023) Martínez Mora, Mª del Carmen; Cuesta Rubio, Natalia; Sanz Miguel, Mª del Carmen; Morales García, José Ángel; Giné Domínguez, Elena; Gómez del Moral Martín Consuegra, Manuel; Cortegano Jimeno, María Isabel; López Moreno, José Antonio; Buhler, Kora Mareen Katharina; López Gómez, Ana; Jiménez Canales, Francisco Javier; Calleja Conde, Javier; Echeverry Alzate, Víctor Alfonso; Grijota Martínez, Mª CarmenEl presente proyecto ha desarrollado una actividad de aprendizaje basado en juegos (Olimpiada de Histología) para asentar los contenidos de Histología Humana en el grado en Medicina, mediante una experiencia de aprendizaje activo divertida y motivadora. Item Gut microbiota and voluntary alcohol consumption(Translational Psychiatry, 2022) Segovia Rodríguez, Lucía; Echeverry Alzate, Víctor; Rincón Pérez. Irene; Calleja Conde, Javier; Buhler, Kora Mareen Katharina; Giné Domínguez, Elena; Albert, J.; Hinojosa Poveda, José Antonio; Huertas Rodríguez, Evelio; Gómez Gallego, Felix; Bressa, C.; Rodríguez De Fonseca, Fernando Antonio; López Moreno, José Antoniolcohol is part of the usual diet of millions of individuals worldwide. However, not all individuals who drink alcohol experience the same effects, nor will everyone develop an alcohol use disorder. Here we propose that the intestinal microbiota (IMB) helps explain the different consumption patterns of alcohol among individuals. 507 humans participated in this study and alcohol consumption and IMB composition were analyzed. On the other hand, in 80 adult male Wistar rats, behavioral tests, alcohol intoxication, fecal transplantation, administration of antibiotics and collection of fecal samples were performed. For identification and relative quantification of bacterial taxa was used the bacterial 16 S ribosomal RNA gene. In humans, we found that heavy episodic drinking is associated with a specific stool type phenotype (type 1, according to Bristol Stool Scale; p < 0.05) and with an increase in the abundance of Actinobacteria (p < 0.05). Next, using rats, we demonstrate that the transfer of IMB from alcohol-intoxicated animals causes an increase in voluntary alcohol consumption in transplant-recipient animals (p < 0.001). The relative quantification data indicate that the genus Porphyromonas could be associated with the effect on voluntary alcohol consumption. We also show that gut microbiota depletion by antibiotics administration causes a reduction in alcohol consumption (p < 0.001) and altered the relative abundance of relevant phyla such as Firmicutes, Bacteroidetes or Cyanobacteria (p < 0.05), among others. Benjamini-Hochberg false discovery rate (FDR) correction was performed for multiple comparisons. These studies reveal some of the consequences of alcohol on the IMB and provide evidence that manipulation of IMB may alter voluntary alcohol consumption.Item Red Bull® energy drink increases consumption of higher concentrations of alcohol(Addiction Biology, 2018) Roldán, Marta; Echeverry-Alzate, Victor; Buhler, Kora Mareen Katharina; Sánchez-Diez, Israel J; Calleja Conde, Javier; Olmos, Pedro; Boehm, Stephen L; Maldonado, Rafael; Rodríguez De Fonseca, Fernando Antonio; Santiago, Catalina; Gómez Gallego, Felix; Giné Domínguez, Elena; López Moreno, José AntonioMixing alcohol with caffeinated energy drinks is a common practice, especially among young people. In humans, the research on this issue has mainly focused on the use of the mass-marketed energy drinks themselves, whereas in animal models, it has focused on the individual effects of their active ingredients (i.e. caffeine). Here, we have characterized how Red Bull®, one of the most consumed caffeinated energy drink worldwide, modulates operant alcohol self-administration in Wistar rats. We found that animals readily and steadily responded for Red Bull (mean: 90 responses, 30 minutes and fixed-ratio 1), which was accompanied by locomotor stimulating effects (26 percent increase). The higher the concentration of alcohol (3–20 percent), the higher the consumption of alcohol (g/kg) and associated blood alcohol levels (91.76 percent) in the mixed Red Bull–alcohol group (60 percent increase). Blood caffeine levels in the Red Bull group were 4.69 μg/ml and 1.31 μg/ml in the Red Bull–alcohol group after the 30-minute session. Because Red Bull also contains 11 percent sucrose, we examined the time course of blood glucose as well as insulin and corticosterone. The correlation between intake of Red Bull and blood glucose levels was higher at 90 minutes than 5 minutes after its consumption, and there was no relationship with blood insulin or blood corticosterone levels. Red Bull did not alter extinction and reacquisition of responding for alcohol nor did it affect relapse-like drinking. Overall, our results suggest that Red Bull might be a vulnerability factor to develop alcoholism given that it intensifies the consumption of higher concentrations of alcohol.Item Adult-onset hypothyroidism increases ethanol consumption(Psychopharmacology, 2019) Echeverry-Alzate, Victor; Buhler, Kora Mareen Katharina; Calleja Conde, Javier; Huertas Rodríguez, Evelio; Maldonado, Rafael; Rodríguez De Fonseca, Fernando Antonio; Santiago, Catalina; Gómez-Gallego, Santiago; Santos Montes, Gregorio Ángel; Giné Domínguez, Elena; López Moreno, José AntonioRationale Only in Europe it can be estimated that more than 20 million of people would be affected by hypothyroidism in some moment of their life. Given that ethanol consumption is so frequent, it would be reasonable to ask what the consequences of ethanol consumption in those individuals affected by hypothyroidism are. Objectives To study the interaction between hypothyroidism and ethanol consumption. Methods We study ethanol consumption in a rat model of methyl-mercaptoimidazole-induced-adult-onset hypothyroidism and thyroid T4/T3 hormone supplementation. Also, we studied the effects of ethanol on motor activity, memory, and anxiety. Results We found that hypothyroidism increased the voluntary ethanol consumption and that this was enhanced by thyroid hormone supplementation. Hypothyroidism was associated with motor hyperactivity which was prevented either by T4/T3 supplementation or ethanol. The relationship between hypothyroidism, ethanol, and anxiety was more complex. In an anxiogenic context, hypothyroidism and T4/T3 supplementation would increase immobility, an anxiety-like behavior, while in a less anxiogenic context would decrease rearing, a behavior related to anxiety. Regarding memory, acute ethanol administration did not alter episodic-like memory in hypothyroid rats. Gene expression of enzymes involved in the metabolism of ethanol, i.e., Adh1 and Aldh2, were altered by hypothyroidism and T4/T3 supplementation. Conclusions Our results suggest that hypothyroid patients would need personalized attention in terms of ethanol consumption. In addition, they point that it would be useful to embrace the thyroid axis in the study of ethanol addiction, including as a possible therapeutic target for the treatment of alcoholism and its comorbid disordersItem The Immune System through the Lens of Alcohol Intake and Gut Microbiota(International Journal of Molecular Sciences, 2021) Calleja Conde, Javier; Echeverry Alzate, Víctor; Buhler, Kora Mareen Katharina; Durán González, Pedro; Morales García, José Ángel; Segovia Rodríguez, Lucía; Rodríguez De Fonseca, Fernando Antonio; Giné Domínguez, Elena; López Moreno, José AntonioThe human gut is the largest organ with immune function in our body, responsible for regulating the homeostasis of the intestinal barrier. A diverse, complex and dynamic population of microorganisms, called microbiota, which exert a significant impact on the host during homeostasis and disease, supports this role. In fact, intestinal bacteria maintain immune and metabolic homeostasis, protecting our organism against pathogens. The development of numerous inflammatory disorders and infections has been linked to altered gut bacterial composition or dysbiosis. Multiple factors contribute to the establishment of the human gut microbiota. For instance, diet is considered as one of the many drivers in shaping the gut microbiota across the lifetime. By contrast, alcohol is one of the many factors that disrupt the proper functioning of the gut, leading to a disruption of the intestinal barrier integrity that increases the permeability of the mucosa, with the final result of a disrupted mucosal immunity. This damage to the permeability of the intestinal membrane allows bacteria and their components to enter the blood tissue, reaching other organs such as the liver or the brain. Although chronic heavy drinking has harmful effects on the immune system cells at the systemic level, this review focuses on the effect produced on gut, brain and liver, because of their significance in the link between alcohol consumption, gut microbiota and the immune system.Item Nalmefene is effective at reducing alcohol seeking, treating alcohol-cocaine interactions and reducing alcohol-induced histone deacetylases gene expression in blood(British Journal of Pharmacology, 2016) Calleja Conde, Javier; Echeverry Alzate, Víctor; Giné Domínguez, Elena; Buhler, Kora Mareen Katharina; Nadal, Roser; Maldonado, Rafael; Rodríguez De Fonseca, Fernando Antonio; Gual, Antoni; López Moreno, José AntonioBackground and Purpose The opioid antagonist nalmefene (selincro®) was approved for alcohol-related disorders by the European Medicines Agency in 2013. However, there have been no studies regarding the effectiveness of nalmefene when alcohol is used in combination with cocaine. Experimental Approach Using operant alcohol self-administration in Wistar rats and qRT-PCR, we evaluated (i) the dose–response curve for s.c. and p.o. nalmefene; (ii) the effects of nalmefene with increasing concentrations of alcohol; (iii) the efficacy of nalmefene on cocaine-potentiated alcohol responding; and (iv) the gene expression profiles of histone deacetylases (Hdac1–11) in peripheral blood in vivo and in the prefrontal cortex, heart, liver and kidney post mortem. Key Results S.c. (0.01, 0.05, 0.1 mg·kg−1) and p.o. (10, 20, 40 mg·kg−1) nalmefene dose-dependently reduced alcohol-reinforced responding by up to 50.3%. This effect of nalmefene was not dependent on alcohol concentration (10, 15, 20%). Cocaine potentiated alcohol responding by approximately 40% and nalmefene (0.05 mg·kg−1) reversed this effect of cocaine. Alcohol increased Hdac gene expression in blood and nalmefene prevented the increases in Hdacs 3, 8, 5, 7, 9, 6 and 10. In the other tissues, alcohol and nalmefene either did not alter the gene expression of Hdacs, as in the prefrontal cortex, or a tissue-Hdac-specific effect was observed. Conclusions and Implications Nalmefene might be effective as a treatment for alcohol-dependent patients who also use cocaine. Also, the expression of Hdacs in peripheral blood might be useful as a biomarker of alcohol use and drug response.Item Histone Deacetylase Gene Expression Following Binge Alcohol Consumption in Rats and Humans(Alcoholism: Clinical and Experimental Research, 2015) López Moreno, José Antonio; Marcos, Miguel; Calleja Conde, Javier; Echeverry-Alzate, Victor; Buhler, Kora M.; Costa-Alba, Pilar; Bernardo, Edgar; Laso, Francisco Javier; Rodríguez de Fonseca, Fernando; Nadal, Rose; Viveros, María Paz; Maldonado, Rafael; Giné Domínguez, ElenaBackground: Alcohol binge drinking is one of the most common patterns of excessive alcohol use and recent data would suggest that histone deacetylases (HDACs) gene expression profiling could be useful as a biomarker for psychiatric disorders. Methods: This study aimed to characterize the gene expression patterns of Hdac 1–11 in samples of rat peripheral blood, liver, heart, prefrontal cortex, and amygdala following repeated binge alcohol consumption and to determine the parallelism of Hdac gene expression between rats and humans in peripheral blood. To accomplish this goal, we examined Hdac gene expression following 1, 4, or 8 alcohol binges (3 g/kg, orally) in the rat, in patients who were admitted to the hospital emergency department for acute alcohol intoxication, and in rats trained in daily operant alcohol self-administration. Results: We primarily found that acute alcohol binging reduced gene expression (Hdac1–10) in the peripheral blood of alcohol-na€ıve rats and that this effect was attenuated following repeated alcohol binges. There was also a reduction of Hdac gene expression in the liver (Hdac2,4,5), whereas there was increased expression in the heart (Hdac1,7,8) and amygdala (Hdac1,2,5). Additionally, increased blood alcohol concentrations were measured in rat blood at 1 to 4 hours following repeated alcohol binging, and the only group that developed hepatic steotosis (fatty liver) were those animals exposed to 8 alcohol binge events. Finally, both binge consumption of alcohol in humans and daily operant alcohol self-administration in rats increased Hdac gene expression in peripheral blood. Conclusions: Our results suggest that increases in HDAC gene expression within the peripheral blood are associated with chronic alcohol consumption, whereas HDAC gene expression is reduced following initial exposure to alcohol.Item The genetics of self-reported trait impulsivity: Contribution of catecholaminergic gene variants in European ancestry individuals(Personality and Individual Differences, 2022) Bühler, Kora Mareen; Rincón Pérez, Irene; Calleja Conde, Javier; Albert, Jacobo; Hinojosa Poveda, José Antonio; Giné Domínguez, Elena; Echeverry lzate, Víctor; López Moreno, José Antonio; Huertas Rodríguez, EvelioIncreased trait impulsivity is a core element in several mental disorders. Given the durable and consistent nature of trait impulsivity, studies have explored its relation to stable biological measures. Variation in catecholaminergic neurotransmission by genetic variants could be one of these biological substrates. Here, 905 participants of European-ancestry completed the Barratt Impulsiveness Scale–11 and were genotyped in three single nucleotide polymorphisms related to catecholaminergic neurotransmission: the DRD2/ ANKK Taq1A, the C957T DRD2 and the Val158Met of the COMT gene. We found significant main effects of Val158Met and C957T on BIS-11 score. Also, interactions with gender were significant in both SNPs with a tendency to slightly different genotype and allele associations with the BIStotal score between male and female participants. Whereas in females, higher impulsivity scores were obtained by participants with the Val158Met heterozygous genotype (Met/Val), data indicate a trend towards a higher impulsivity score in male Val-allele carriers. In the case of C957T, only a tentative association between male Tallele carriers and higher impulsivity scores in comparison to CC genotype carriers could be established. No significant associations were found between BIS-11 and Taq1A. We provide further evidence for a gender-specific implication of Val158Met and C957T in trait impulsivity.