Person:
Giné Domínguez, Elena

First Name

Elena

Last Name

Giné Domínguez

URI

https://hdl.handle.net/20.500.14352/79657

Affiliation

Universidad Complutense de Madrid

Faculty / Institute

Medicina

Department

Biología Celular

Area

Biología Celular

Identifiers

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Now showing 1 - 10 of 11

Project number: PIMCD207
Desarrollo e implementación de una herramienta de evaluación en los tribunales de los Trabajos de Fin de Grado y Fin de Máster en las ciencias de la salud
(2016) Sanz Miguel, María del Carmen; Giné Domínguez, Elena; Navas Hernández, Ángeles; Hurtado Carneiro, Verónica; Benito Miguel, Marta; Gutiérrez Nogués, Ángel; Dongil Sánchez, Pilar; Pérez García, Ana
Red Bull® energy drink increases consumption of higher concentrations of alcohol
(Addiction Biology, 2018) Roldán, Marta; Echeverry-Alzate, Victor; Buhler, Kora Mareen Katharina; Sánchez-Diez, Israel J; Calleja Conde, Javier; Olmos, Pedro; Boehm, Stephen L; Maldonado, Rafael; Rodríguez De Fonseca, Fernando Antonio; Santiago, Catalina; Gómez Gallego, Felix; Giné Domínguez, Elena; López Moreno, José Antonio
Mixing alcohol with caffeinated energy drinks is a common practice, especially among young people. In humans, the research on this issue has mainly focused on the use of the mass-marketed energy drinks themselves, whereas in animal models, it has focused on the individual effects of their active ingredients (i.e. caffeine). Here, we have characterized how Red Bull®, one of the most consumed caffeinated energy drink worldwide, modulates operant alcohol self-administration in Wistar rats. We found that animals readily and steadily responded for Red Bull (mean: 90 responses, 30 minutes and fixed-ratio 1), which was accompanied by locomotor stimulating effects (26 percent increase). The higher the concentration of alcohol (3–20 percent), the higher the consumption of alcohol (g/kg) and associated blood alcohol levels (91.76 percent) in the mixed Red Bull–alcohol group (60 percent increase). Blood caffeine levels in the Red Bull group were 4.69 μg/ml and 1.31 μg/ml in the Red Bull–alcohol group after the 30-minute session. Because Red Bull also contains 11 percent sucrose, we examined the time course of blood glucose as well as insulin and corticosterone. The correlation between intake of Red Bull and blood glucose levels was higher at 90 minutes than 5 minutes after its consumption, and there was no relationship with blood insulin or blood corticosterone levels. Red Bull did not alter extinction and reacquisition of responding for alcohol nor did it affect relapse-like drinking. Overall, our results suggest that Red Bull might be a vulnerability factor to develop alcoholism given that it intensifies the consumption of higher concentrations of alcohol.
Project number: 168
La figura de Cajal: Vacuna contra la neurofobia y estímulo de vocaciones investigadoras para los estudiantes de medicina
(2019) Sanz Miguel, María del Carmen; Martínez Mora, María del Carmen; Giné Domínguez, Elena; Morales García, José Angel; López Moreno, José Antonio; Zemanova, Marketa; Pérez Martínez, David A.; Jiménez Canales, Francisco Javier
Proponemos actividades centradas en D. Santiago Ramón y Cajal dirigidas a prevenir la neurofobia (miedo a las neurociencias y a la neurología clínica) entre los estudiantes de Medicina y fomentar su interés por la investigación biomédica traslacional.
Long-term effects of intermittent adolescent alcohol exposure in male and female rats
(Frontiers in Behavioral Neuroscience, 2017) Marco López, Eva María; Peñasco, Sara; Hernández, María Donina; Gil, Anabel; Borcel, Erika; Moya, Marta; Giné Domínguez, Elena; López Moreno, José Antonio; Guerri, Consuelo; López Gallardo, Meritxell; Rodríguez de Fonseca, Fernando
Alcohol is a serious public health concern that has a differential impact on individuals depending upon age and sex. Patterns of alcohol consumption have recently changed: heavy episodic drinking—known as binge-drinking—has become most popular among the youth. Herein, we aimed to investigate the consequences of intermittent adolescent alcohol consumption in male and female animals. Thus, Wistar rats were given free access to ethanol (20% in drinking water) or tap water for 2-h sessions during 3 days, and for an additional 4-h session on the 4th day; every week during adolescence, from postnatal day (pnd) 28–52. During this period, animals consumed a moderate amount of alcohol despite blood ethanol concentration (BEC) did not achieve binge-drinking levels. No withdrawal signs were observed: no changes were observed regarding anxiety-like responses in the elevated plus-maze or plasma corticosterone levels (pnd 53–54). In the novel object recognition (NOR) test (pnd 63), a significant deficit in recognition memory was observed in both male and female rats. Western Blot analyses resulted in an increase in the expression of synaptophysin in the frontal cortex (FC) of male and female animals, together with a decrease in the expression of the CB2R in the same brain region. In addition, adolescent alcohol induced, exclusively among females, a decrease in several markers of dopaminergic and serotonergic neurotransmission, in which epigenetic mechanisms, i.e., histone acetylation, might be involved. Taken together, further research is still needed to specifically correlate sex-specific brain and behavioral consequences of adolescent alcohol exposure.
Project number: 101
Santiago Ramón y Cajal: un modelo de excelencia para desarrollar competencias en el Grado en Medicina
(2018) Martínez Mora, María del Carmen; Giné Domínguez, Elena; Sanz Miguel, María del Carmen; Varas Fajardo, Alberto; Pérez Martínez, David Andrés; Valiño Seoane, Iria; Martínez Murillo, Ricardo; De Castro Soubriet, Fernando
El presente proyecto ha acercado la figura de Santiago Ramón y Cajal a los estudiantes de Medicina como estrategia para el desarrollo de competencias generales, específicas y transversales necesarias para la formación de profesionales competentes. Se han realizado una serie de actividades en torno a nuestro Premio Nobel que han involucrado a docentes, investigadores y clínicos.
Adult-onset hypothyroidism increases ethanol consumption
(Psychopharmacology, 2019) Echeverry-Alzate, Victor; Buhler, Kora Mareen Katharina; Calleja Conde, Javier; Huertas Rodríguez, Evelio; Maldonado, Rafael; Rodríguez De Fonseca, Fernando Antonio; Santiago, Catalina; Gómez-Gallego, Santiago; Santos Montes, Gregorio Ángel; Giné Domínguez, Elena; López Moreno, José Antonio
Rationale Only in Europe it can be estimated that more than 20 million of people would be affected by hypothyroidism in some moment of their life. Given that ethanol consumption is so frequent, it would be reasonable to ask what the consequences of ethanol consumption in those individuals affected by hypothyroidism are. Objectives To study the interaction between hypothyroidism and ethanol consumption. Methods We study ethanol consumption in a rat model of methyl-mercaptoimidazole-induced-adult-onset hypothyroidism and thyroid T4/T3 hormone supplementation. Also, we studied the effects of ethanol on motor activity, memory, and anxiety. Results We found that hypothyroidism increased the voluntary ethanol consumption and that this was enhanced by thyroid hormone supplementation. Hypothyroidism was associated with motor hyperactivity which was prevented either by T4/T3 supplementation or ethanol. The relationship between hypothyroidism, ethanol, and anxiety was more complex. In an anxiogenic context, hypothyroidism and T4/T3 supplementation would increase immobility, an anxiety-like behavior, while in a less anxiogenic context would decrease rearing, a behavior related to anxiety. Regarding memory, acute ethanol administration did not alter episodic-like memory in hypothyroid rats. Gene expression of enzymes involved in the metabolism of ethanol, i.e., Adh1 and Aldh2, were altered by hypothyroidism and T4/T3 supplementation. Conclusions Our results suggest that hypothyroid patients would need personalized attention in terms of ethanol consumption. In addition, they point that it would be useful to embrace the thyroid axis in the study of ethanol addiction, including as a possible therapeutic target for the treatment of alcoholism and its comorbid disorders
Project number: 48
Elaboración de blogs como herramienta virtual de aprendizaje y trabajo en equipo
(2017) Sanz Miguel, Carmen; Martínez Mora, Carmen; Giné Domínguez, Elena; López Moreno, José Antonio; Hurtado Carneiro, Verónica; Lamana Rodríguez, Amalia; Valiño Seoane, Iria; Triguero Martínez, Ana
Este proyecto de innovación se enmarca dentro de las actividades académicas no presenciales. Se trata de la elaboración de blogs como herramienta virtual de aprendizaje. Los blogs son un recurso de aprendizaje individual o grupal, de gran versatilidad y dinamización del aula. Además, promueve el desarrollo de competencias generales, específicas y transversales que los alumnos deben adquirir durante su formación. Creemos que su aplicación es especialmente interesante en asignaturas como “Bases Celulares de la Genética Humana” impartida en primero de Medicina, donde los alumnos se encuentran con una dificultad añadida a la complejidad de sus fundamentos: unos conceptos complejos con una terminología muy específica para asimilarlos. Los futuros médicos deberán ser capaces, de explicar con un lenguaje sencillo y comprensible a los pacientes la implicación de la genética en las patologías. Consideramos que el uso de blogs está especialmente indicado en este caso para facilitar la adquisición de esta competencia específica, además de apoyar el aprendizaje de parte de los contenidos de la asignatura y promover el trabajo en equipo. Esta herramienta aporta a los alumnos las competencias necesarias para su formación integral como profesional de la salud promoviendo el intercambio colaborativo. Es además, un recurso que puede ser utilizado tanto para la evaluación continua, por parte del profesor durante y a la finalización del diseño del blog, así como para la coevaluación entre los alumnos. Por tanto, esta propuesta facilita el aprendizaje creando espacios para ello. En definitiva, crea oportunidades de aprendizaje.
Differential effects of environmental enrichment and isolation housing on the hormonal and neurochemical responses to stress in the prefrontal cortex of the adult rat: relationship to working and emotional memories.
(Journal of Neural Transmission, 2013) Garrido, Pedro; Blas, Marta de; Ronzoni Blázquez, Giacomo; Cordero, Isabel; Antón, María; Giné Domínguez, Elena; Santos Montes, Ángel; Arco González, Alberto del; Segovia Camargo, Gregorio; Mora Teruel, Francisco
The present study was designed to investigate the modulation of the stress responses by the environmental conditions and its putative neurobiological mechanisms. For that an integrative study on the effects of environmental enrichment and isolation housing on (1) the corticosterone, dopamine and acetylcholine responses to acute restraint stress in the prefrontal cortex (PFC) of the awake rat; (2) the mRNA levels of glucocorticoid receptors (GRs) in the PFC, and (3) the behavioral responses to stress, related to the PFC (habituation to a novel environment, spatial-working memory and inhibitory avoidance response) was performed. Male Wistar rats were maintained from 3 to 6 months of age in two different conditions: enriched (EC) or impoverished (IC). Animals were stereotaxically implanted with bilateral guide cannulae in the PFC to perform microdialysis experiments to evaluate the concentrations of corticosterone, dopamine and acetylcholine. EC animals showed lower increases of corticosterone and dopamine but not of acetylcholine than IC animals in the PFC in response to acute restraint stress (20 min). In the PFC, GR mRNA levels showed a trend towards an enhancement in EC animals. EC reduced the days to learn the spatial working memory task (radial-water maze). Spatial working memory, however, was not different between groups in either basal or stress conditions. Inhibitory avoidance response was reduced in EC rats. The changes produced by EC in the neurochemical, neuroendocrine and behavioral parameters evaluated suggest that EC rats could show a better coping during an acute stress challenge.
Nalmefene is effective at reducing alcohol seeking, treating alcohol-cocaine interactions and reducing alcohol-induced histone deacetylases gene expression in blood
(British Journal of Pharmacology, 2016) Calleja Conde, Javier; Echeverry Alzate, Víctor; Giné Domínguez, Elena; Buhler, Kora Mareen Katharina; Nadal, Roser; Maldonado, Rafael; Rodríguez De Fonseca, Fernando Antonio; Gual, Antoni; López Moreno, José Antonio
Background and Purpose The opioid antagonist nalmefene (selincro®) was approved for alcohol-related disorders by the European Medicines Agency in 2013. However, there have been no studies regarding the effectiveness of nalmefene when alcohol is used in combination with cocaine. Experimental Approach Using operant alcohol self-administration in Wistar rats and qRT-PCR, we evaluated (i) the dose–response curve for s.c. and p.o. nalmefene; (ii) the effects of nalmefene with increasing concentrations of alcohol; (iii) the efficacy of nalmefene on cocaine-potentiated alcohol responding; and (iv) the gene expression profiles of histone deacetylases (Hdac1–11) in peripheral blood in vivo and in the prefrontal cortex, heart, liver and kidney post mortem. Key Results S.c. (0.01, 0.05, 0.1 mg·kg−1) and p.o. (10, 20, 40 mg·kg−1) nalmefene dose-dependently reduced alcohol-reinforced responding by up to 50.3%. This effect of nalmefene was not dependent on alcohol concentration (10, 15, 20%). Cocaine potentiated alcohol responding by approximately 40% and nalmefene (0.05 mg·kg−1) reversed this effect of cocaine. Alcohol increased Hdac gene expression in blood and nalmefene prevented the increases in Hdacs 3, 8, 5, 7, 9, 6 and 10. In the other tissues, alcohol and nalmefene either did not alter the gene expression of Hdacs, as in the prefrontal cortex, or a tissue-Hdac-specific effect was observed. Conclusions and Implications Nalmefene might be effective as a treatment for alcohol-dependent patients who also use cocaine. Also, the expression of Hdacs in peripheral blood might be useful as a biomarker of alcohol use and drug response.
Histone Deacetylase Gene Expression Following Binge Alcohol Consumption in Rats and Humans
(Alcoholism: Clinical and Experimental Research, 2015) López Moreno, José Antonio; Marcos, Miguel; Calleja Conde, Javier; Echeverry-Alzate, Victor; Buhler, Kora M.; Costa-Alba, Pilar; Bernardo, Edgar; Laso, Francisco Javier; Rodríguez de Fonseca, Fernando; Nadal, Rose; Viveros, María Paz; Maldonado, Rafael; Giné Domínguez, Elena
Background: Alcohol binge drinking is one of the most common patterns of excessive alcohol use and recent data would suggest that histone deacetylases (HDACs) gene expression profiling could be useful as a biomarker for psychiatric disorders. Methods: This study aimed to characterize the gene expression patterns of Hdac 1–11 in samples of rat peripheral blood, liver, heart, prefrontal cortex, and amygdala following repeated binge alcohol consumption and to determine the parallelism of Hdac gene expression between rats and humans in peripheral blood. To accomplish this goal, we examined Hdac gene expression following 1, 4, or 8 alcohol binges (3 g/kg, orally) in the rat, in patients who were admitted to the hospital emergency department for acute alcohol intoxication, and in rats trained in daily operant alcohol self-administration. Results: We primarily found that acute alcohol binging reduced gene expression (Hdac1–10) in the peripheral blood of alcohol-na€ıve rats and that this effect was attenuated following repeated alcohol binges. There was also a reduction of Hdac gene expression in the liver (Hdac2,4,5), whereas there was increased expression in the heart (Hdac1,7,8) and amygdala (Hdac1,2,5). Additionally, increased blood alcohol concentrations were measured in rat blood at 1 to 4 hours following repeated alcohol binging, and the only group that developed hepatic steotosis (fatty liver) were those animals exposed to 8 alcohol binge events. Finally, both binge consumption of alcohol in humans and daily operant alcohol self-administration in rats increased Hdac gene expression in peripheral blood. Conclusions: Our results suggest that increases in HDAC gene expression within the peripheral blood are associated with chronic alcohol consumption, whereas HDAC gene expression is reduced following initial exposure to alcohol.