Person:
Giné Domínguez, Elena

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First Name
Elena
Last Name
Giné Domínguez
URI
https://hdl.handle.net/20.500.14352/79657
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Medicina
Department
Biología Celular
Area
Biología Celular
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2015 - 20172
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Item Type: Publication × Subject: 591.1 ×

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    Long-term effects of intermittent adolescent alcohol exposure in male and female rats
    (Frontiers in Behavioral Neuroscience, 2017) Marco López, Eva María; Peñasco, Sara; Hernández, María Donina; Gil, Anabel; Borcel, Erika; Moya, Marta; Giné Domínguez, Elena; López Moreno, José Antonio; Guerri, Consuelo; López Gallardo, Meritxell; Rodríguez de Fonseca, Fernando
    Alcohol is a serious public health concern that has a differential impact on individuals depending upon age and sex. Patterns of alcohol consumption have recently changed: heavy episodic drinking—known as binge-drinking—has become most popular among the youth. Herein, we aimed to investigate the consequences of intermittent adolescent alcohol consumption in male and female animals. Thus, Wistar rats were given free access to ethanol (20% in drinking water) or tap water for 2-h sessions during 3 days, and for an additional 4-h session on the 4th day; every week during adolescence, from postnatal day (pnd) 28–52. During this period, animals consumed a moderate amount of alcohol despite blood ethanol concentration (BEC) did not achieve binge-drinking levels. No withdrawal signs were observed: no changes were observed regarding anxiety-like responses in the elevated plus-maze or plasma corticosterone levels (pnd 53–54). In the novel object recognition (NOR) test (pnd 63), a significant deficit in recognition memory was observed in both male and female rats. Western Blot analyses resulted in an increase in the expression of synaptophysin in the frontal cortex (FC) of male and female animals, together with a decrease in the expression of the CB2R in the same brain region. In addition, adolescent alcohol induced, exclusively among females, a decrease in several markers of dopaminergic and serotonergic neurotransmission, in which epigenetic mechanisms, i.e., histone acetylation, might be involved. Taken together, further research is still needed to specifically correlate sex-specific brain and behavioral consequences of adolescent alcohol exposure.
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    Histone Deacetylase Gene Expression Following Binge Alcohol Consumption in Rats and Humans
    (Alcoholism: Clinical and Experimental Research, 2015) López Moreno, José Antonio; Marcos, Miguel; Calleja Conde, Javier; Echeverry-Alzate, Victor; Buhler, Kora M.; Costa-Alba, Pilar; Bernardo, Edgar; Laso, Francisco Javier; Rodríguez de Fonseca, Fernando; Nadal, Rose; Viveros, María Paz; Maldonado, Rafael; Giné Domínguez, Elena
    Background: Alcohol binge drinking is one of the most common patterns of excessive alcohol use and recent data would suggest that histone deacetylases (HDACs) gene expression profiling could be useful as a biomarker for psychiatric disorders. Methods: This study aimed to characterize the gene expression patterns of Hdac 1–11 in samples of rat peripheral blood, liver, heart, prefrontal cortex, and amygdala following repeated binge alcohol consumption and to determine the parallelism of Hdac gene expression between rats and humans in peripheral blood. To accomplish this goal, we examined Hdac gene expression following 1, 4, or 8 alcohol binges (3 g/kg, orally) in the rat, in patients who were admitted to the hospital emergency department for acute alcohol intoxication, and in rats trained in daily operant alcohol self-administration. Results: We primarily found that acute alcohol binging reduced gene expression (Hdac1–10) in the peripheral blood of alcohol-na€ıve rats and that this effect was attenuated following repeated alcohol binges. There was also a reduction of Hdac gene expression in the liver (Hdac2,4,5), whereas there was increased expression in the heart (Hdac1,7,8) and amygdala (Hdac1,2,5). Additionally, increased blood alcohol concentrations were measured in rat blood at 1 to 4 hours following repeated alcohol binging, and the only group that developed hepatic steotosis (fatty liver) were those animals exposed to 8 alcohol binge events. Finally, both binge consumption of alcohol in humans and daily operant alcohol self-administration in rats increased Hdac gene expression in peripheral blood. Conclusions: Our results suggest that increases in HDAC gene expression within the peripheral blood are associated with chronic alcohol consumption, whereas HDAC gene expression is reduced following initial exposure to alcohol.