Differential Apoptotic Effects of Bee Product Mixtures on Normal and Cancer Hepatic Cells

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dc.contributor.authorSánchez Martín, Vanesa
dc.contributor.authorMorales Gómez, Paloma
dc.contributor.authorIriondo De Hond, Amaia
dc.contributor.authorFernández Hospital, Xavier
dc.contributor.authorFernández Álvarez, Manuela
dc.contributor.authorHierro Paredes, Eva
dc.contributor.authorHaza Duaso, Ana Isabel
dc.contributor.editorGan, Ren-You
dc.date.accessioned2023-12-19T17:08:19Z
dc.date.available2023-12-19T17:08:19Z
dc.date.issued2023-03-02
dc.description.abstractMost effective anticancer drugs normally generate considerable cytotoxicity in normal cells; therefore, the preferential activation of apoptosis in cancer cells and the reduction of toxicity in normal cells is a great challenge in cancer research. Natural products with selective anticancer properties used as complementary medicine can help to achieve this goal. The aim of the present study was to analyze the effect of the addition of bee products [propolis (PR) or royal jelly (RJ) or propolis and royal jelly (PR+RJ), 2–10%] to thyme (TH) and chestnut honeys (CH) on the differential anticancer properties, mainly the cytotoxic and pro-apoptotic effects, in normal and cancer hepatic cells. The cytotoxic effects of samples were analyzed using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay (0–250 mg/mL) and the effects on apoptosis were analyzed using cell cycle analysis, TdT-dUTP terminal nick-end labeling (TUNEL) assay, DR5 (Death Receptor 5) and BAX (BCL-2-Associated X) activation, and caspases 8, 9, and 3 activities. Both honey samples alone and honey mixtures had no or very little apoptotic effect on normal cells. Antioxidant honey mixtures enhanced the apoptotic capacity of the corresponding honey alone via both extrinsic and intrinsic pathways. Of all the samples, chestnut honey enriched with 10% royal jelly and 10% propolis (sample 14, CH+10RJ+10PR) showed the highest apoptotic effect on tumor liver cells. The enrichment of monofloral honey with bee products could be used together with conventional anticancer treatments as a dietary supplement without side effects. On the other hand, it could be included in the diet as a natural sweetener with high added value.
dc.description.departmentSección Dptal. de Nutrición y Ciencia de los Alimentos (Veterinaria)
dc.description.departmentSección Deptal. de Farmacia Galénica y Tecnología Alimentaria (Veterinaria)
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia e Innovación
dc.description.statuspub
dc.identifier.citationSánchez-Martín, V.; Morales, P.; Iriondo-DeHond, A.; Hospital, X.F.; Fernández, M.; Hierro, E.; Haza, A.I. Differential Apoptotic Effects of Bee Product Mixtures on Normal and Cancer Hepatic Cells. Antioxidants 2023, 12, 615. https:// doi.org/10.3390/antiox12030615
dc.identifier.doi10.3390/antiox12030615
dc.identifier.essn2076-3921
dc.identifier.officialurlhttps://www.mdpi.com/2076-3921/12/3/615
dc.identifier.urihttps://hdl.handle.net/20.500.14352/91544
dc.issue.number3
dc.journal.titleAntioxidants
dc.language.isoeng
dc.page.initial615
dc.publisherMDPI
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-097549-B-I00/ES/ESTRATEGIAS PARA REDUCIR O REEMPLAZAR ADITIVOS ALIMENTARIOS (CONSERVANTES Y ANTIOXIDANTES) EN PRODUCTOS CARNICOS CURADOS/
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.keywordAntioxidant honey mixtures
dc.subject.keywordApoptosis
dc.subject.keywordBee products
dc.subject.keywordCancer
dc.subject.keywordCytotoxicity
dc.subject.keywordHoney
dc.subject.keywordPropolis
dc.subject.keywordRoyal jelly
dc.subject.ucmCiencias Biomédicas
dc.subject.ucmDietética y nutrición (Farmacia)
dc.subject.unesco31 Ciencias Agrarias
dc.subject.unesco3212 Salud Publica
dc.subject.unesco3309 Tecnología de Los Alimentos
dc.subject.unesco3214 Toxicología
dc.titleDifferential Apoptotic Effects of Bee Product Mixtures on Normal and Cancer Hepatic Cells
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number12
dspace.entity.typePublication
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