Delayed alveolar clearance of nanoparticles through control of coating composition and interaction with lung surfactant protein A

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dc.contributor.authorCarregal Romero, Susana
dc.contributor.authorGroult, Hugo
dc.contributor.authorCañadas Benito, Olga
dc.contributor.authorA-Gonzalez, Noelia
dc.contributor.authorLechuga Vieco, Ana Victoria
dc.contributor.authorGarcía Fojeda, Belén
dc.contributor.authorHerranz, Fernando
dc.contributor.authorPellico, Juan
dc.contributor.authorHidalgo, Andrés
dc.contributor.authorCasals, Cristina
dc.contributor.authorRuiz Cabello, Jesús
dc.date.accessioned2023-06-16T14:19:26Z
dc.date.available2023-06-16T14:19:26Z
dc.date.issued2021-11-14
dc.descriptionCRUE-CSIC (Acuerdos Transformativos 2021)
dc.description.abstractThe coating composition of nanomedicines is one of the main features in determining the medicines' fate, clearance, and immunoresponse in the body. To highlight the coatings' impact in pulmonary administration, two micellar superparamagnetic iron oxide nanoparticles (SPION) were compared. These nanoparticles are similar in size and charge but have different coatings: either phosphatidylcholine (PC-SPION) or bovine serum albumin (BSA-SPION). The aim of the study was to increase the understanding of the nano-bio interaction with the cellular and non-cellular components of the lung and underline valuable coatings either for local lung-targeted drug delivery in theranostic application or patient-friendly route systemic administration. PC-SPION and BSA-SPION were deposited in the alveoli by in vivo instillation and, despite the complexity of imaging the lung, SPION were macroscopically visualized by MRI. Impressively, PC-SPION were retained within the lungs for at least a week, while BSA-SPION were cleared more rapidly. The different lung residence times were confirmed by histological analysis and supported by a flow cytometry analysis of the SPION interactions with different myeloid cell populations. To further comprehend the way in which these nanoformulations interact with lung components at the molecular level, we used fluorescence spectroscopy, turbidity measurements, and dynamic light scattering to evaluate the interactions of the two SPION with surfactant protein A (SP-A), a key protein in setting up the nanoparticle behavior in the alveolar fluid. We found that SP-A induced aggregation of PC-SPION, but not BSA-SPION, which likely caused PC-SPION retention in the lung without inducing inflammation. In conclusion, the two SPION show different outcomes from interaction with SP-A leading to distinctive fate in the lung. PC-SPION hold great promise as imaging and theranostic agents when prolonged pulmonary drug delivery is required.
dc.description.departmentDepto. de Bioquímica y Biología Molecular
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia e Innovación (MICINN)
dc.description.sponsorshipGobierno Vasco.ELKARTEK Program
dc.description.sponsorshipBBVA Foundation
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/70127
dc.identifier.doi10.1016/j.msec.2021.112551
dc.identifier.issn0928-4931
dc.identifier.officialurlhttps://doi.org/10.1016/j.msec.2021.112551
dc.identifier.urihttps://hdl.handle.net/20.500.14352/4680
dc.journal.titleMaterials Science & Engineering C
dc.language.isoeng
dc.page.initial112551
dc.publisherElsevier
dc.relation.projectID(Grants SAF2017-84494-C2-R and MAT2015-65184-C2-2-R to J.R-C and SAF2015-65307-R and RTI2018-094355-BI00 to C.C.)
dc.relation.projectID(Grant No. KK-2019/bmG19 to J.R-C)
dc.relation.projectID(PR[18]_BIO_IMG_0008 to J.R-C)
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subject.cdu577.1
dc.subject.keywordMicellar superparamagnetic iron oxide
dc.subject.keywordnanoparticles
dc.subject.keywordPulmonary administration
dc.subject.keywordMagnetic resonance imaging
dc.subject.keywordLung clearance
dc.subject.keywordSurfactant protein A
dc.subject.ucmBiología molecular (Química)
dc.titleDelayed alveolar clearance of nanoparticles through control of coating composition and interaction with lung surfactant protein A
dc.typejournal article
dspace.entity.typePublication
relation.isAuthorOfPublication25a89a2f-a381-4f15-9a6b-59430ee96a63
relation.isAuthorOfPublication.latestForDiscovery25a89a2f-a381-4f15-9a6b-59430ee96a63
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