Electrochemical magnetoimmunosensor for the ultrasensitive determination of interleukin-6 in saliva and urine using poly-HRP streptavidin conjugates as labels for signal amplification

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dc.contributor.authorOjeda, I
dc.contributor.authorMoreno Guzmán, María
dc.contributor.authorGonzález Cortés, Araceli
dc.contributor.authorYáñez Sedeño, Paloma
dc.contributor.authorPingarrón Carrazón, José Manuel
dc.date.accessioned2023-06-19T15:11:50Z
dc.date.available2023-06-19T15:11:50Z
dc.date.issued2014
dc.description.abstractA novel magnetoimmunosensor design for interleukin-6 (IL-6) which involved the covalent immobilization of anti-IL-6 antibodies onto carboxyl-functionalized magnetic microparticles and a sandwich-type immunoassay with signal amplification using poly-HRP-streptavidin conjugates is reported. All the variables concerning the preparation and the electroanalytical performance of the immunosensor were optimized. The use of poly-HRP-strept conjugates as enzymatic labels instead of conventional HRP-strept allowed enhanced signal-to-blank current ratios to be obtained. A linear calibration plot between the measured steady-state current and the log of IL-6 concentration was achieved in the 1.75 to 500 pg/mL range, which was not feasible when using HRP-strep as label. A limit of detection of 0.39 pg/mL IL-6 was obtained. The antiIL-6-MB conjugates exhibited an excellent storage stability providing amperometric responses with no significant loss during at least 36 days. The magnetoimmunosensor showed also an excellent selectivity against potentially interfering substances. The immunosensor was used to determine IL-6 in urine samples spiked at three different concentration levels with clinical relevance. Moreover, IL-6 was measured in three different saliva samples corresponding to a periodontitis patient, a smoker volunteer, and a non-smoker volunteer. The obtained results were statistically in agreement with those provided by a commercial ELISA kit.
dc.description.departmentDepto. de Química Analítica
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía y Competitividad (MINECO)
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/44805
dc.identifier.doi10.1007/s00216-014-8055-6
dc.identifier.issn1618-2642 (Print) 1618-2650 (Web)
dc.identifier.officialurlhttps://link.springer.com/article/10.1007/s00216-014-8055-6
dc.identifier.urihttps://hdl.handle.net/20.500.14352/35525
dc.journal.titleAnalytical and Bioanalytical Chemistry
dc.language.isoeng
dc.page.final6371
dc.page.initial6363
dc.publisherSpringer
dc.relation.projectID(Project CTQ 2012–35041)
dc.rights.accessRightsrestricted access
dc.subject.cdu543
dc.subject.keywordInterleukin-6
dc.subject.keywordElectrochemical magnetoimmunosensor
dc.subject.keywordSignal amplification
dc.subject.keywordPoly-HRP-streptavidin
dc.subject.keywordUrine
dc.subject.keywordSaliva
dc.subject.ucmQuímica analítica (Química)
dc.subject.unesco2301 Química Analítica
dc.titleElectrochemical magnetoimmunosensor for the ultrasensitive determination of interleukin-6 in saliva and urine using poly-HRP streptavidin conjugates as labels for signal amplification
dc.typejournal article
dc.volume.number406
dspace.entity.typePublication
relation.isAuthorOfPublication96968530-a80d-4987-a341-f4a96697f66c
relation.isAuthorOfPublication8a730734-cdf5-4b59-b017-12e2320c872e
relation.isAuthorOfPublication8808f99c-5f56-4562-839a-517626c76dad
relation.isAuthorOfPublication.latestForDiscovery96968530-a80d-4987-a341-f4a96697f66c
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