The pathogenic Th profile of human activated memory Th cells in early rheumatoid arthritis can be modulated by VIP

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dc.contributor.authorJimeno Lumeras, Rebeca Gema
dc.contributor.authorPérez Gomáriz, Rosa María
dc.contributor.authorGarín, Marina I.
dc.contributor.authorGutiérrez Cañas, Irene
dc.contributor.authorGonzález Álvaro, Isidoro
dc.contributor.authorCarrión Caballo, Mar
dc.contributor.authorGalindo, María
dc.contributor.authorLeceta Martínez, Javier
dc.contributor.authorJuarranz Moratilla, Yasmina
dc.date.accessioned2023-06-19T13:32:45Z
dc.date.available2023-06-19T13:32:45Z
dc.date.issued2015
dc.description.abstractOur aim is to study the behavior of memory Th cells (Th17, Th17/1, and Th1 profiles) from early rheumatoid arthritis (eRA) patients after their in vitro activation/expansion to provide information about its contribution to RA chronicity. Moreover, we analyzed the potential involvement of vasoactive intestinal peptide (VIP) as an endogenous healing mediator. CD4+CD45RO+ T cells from PBMCs of HD and eRA were activated/expanded in vitro in the presence/absence of VIP. FACS, ELISA, RT-PCR, and immunocytochemistry analyseswere performed. An increase in CCR6+/RORC+ cells and in RORC-proliferating cells and a decrease in T-betproliferating cells and T-bet+/RORC+ cells were shown in eRA. mRNA expression of IL-17, IL-2, RORC, RORA, STAT3, and Tbx21 and protein secretion of IL-17, IFNγ, and GM-CSF were higher in eRA. VIP decreased the mRNA expression of IL-22, IL-2, STAT3, Tbx21, IL-12Rβ2, IL-23R, and IL-21R in HD and it decreased IL-21, IL-2, and STAT3 in eRA. VIP decreased IL-22 and GM-CSF secretion and increased IL-9 secretion in HD and it decreased IL-21 secretion in eRA. VPAC2/VPAC1 ratio expression was increased in eRA. All in all, memory Th cells from eRA patients show a greater proportion of Th17 cells with a pathogenic Th17 and Th17/1 profile compared to HD. VIP is able to modulate the pathogenic profile, mostly in HD. Our results are promising for therapy in the early stages of RA because they suggest that targeting molecules involved in the pathogenic Th17, Th17/1, and Th1 phenotypes and targeting VIP receptors could have a therapeutic effect modulating these subsets.
dc.description.departmentDepto. de Biología Celular
dc.description.facultyFac. de Ciencias Biológicas
dc.description.refereedTRUE
dc.description.sponsorshipInstituto de Salud Carlos III
dc.description.sponsorshipComunidad de Madrid
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/29563
dc.identifier.doi10.1007/s00109-014-1232-4
dc.identifier.issn0946-2716, 1432-1440 (ESSN)
dc.identifier.officialurlhttp://link.springer.com/article/10.1007%2Fs00109-014-1232-4
dc.identifier.urihttps://hdl.handle.net/20.500.14352/33984
dc.issue.number4
dc.journal.titleJournal of Molecular Medicine
dc.language.isoeng
dc.page.final467
dc.page.initial457
dc.publisherSpringer
dc.relation.projectIDPI11/00195, PI12/00758, and RETICS RD08/0075, RD12/0009/0002, RD12/0009/0017
dc.relation.projectIDS2010/ BMD-2350
dc.rightsAtribución-CompartirIgual 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by-sa/3.0/es/
dc.subject.cdu577.175.82
dc.subject.cdu616.72-002
dc.subject.keywordRheumatoid arthritis
dc.subject.keywordTh17
dc.subject.keywordTh1
dc.subject.keywordVasoactive intestinal peptide
dc.subject.keywordVPAC receptors
dc.subject.ucmReumatología
dc.subject.ucmBiología molecular (Biología)
dc.subject.ucmNeurociencias (Biológicas)
dc.subject.unesco3205.09 Reumatología
dc.subject.unesco2415 Biología Molecular
dc.subject.unesco2490 Neurociencias
dc.titleThe pathogenic Th profile of human activated memory Th cells in early rheumatoid arthritis can be modulated by VIP
dc.typejournal article
dc.volume.number93
dspace.entity.typePublication
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relation.isAuthorOfPublication20129605-5bb5-41f6-8dd8-32c0678ecb2f
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relation.isAuthorOfPublication.latestForDiscovery20129605-5bb5-41f6-8dd8-32c0678ecb2f
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