Characterization of Pediococcus acidilactici strains isolated from rainbow trout (Oncorhynchus mykiss, Walbaum) feed and larvae: safety, DNA fingerprinting and bacteriocinogenicity

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dc.contributor.authorDias Araújo, C.
dc.contributor.authorMuñoz Atienza, Estefanía
dc.contributor.authorPoeta, P.
dc.contributor.authorIgrejas, G.
dc.contributor.authorHernández Cruza, Pablo Elpidio
dc.contributor.authorHerranz Sorribes, Carmen
dc.contributor.authorCintas Izarra, Luis Miguel
dc.date.accessioned2024-01-24T10:12:30Z
dc.date.available2024-01-24T10:12:30Z
dc.date.issued2016-05-03
dc.description.abstractThe use of lactic acid bacteria (LAB) as probiotics constitutes an alternative or complementary strategy to chemotherapy and vaccination for disease control in aquaculture. The objectives of this work were (1) the in vitro safety assessment of 8 Pediococcus acidilactici strains isolated from rainbow trout (Oncorhynchus mykiss, Walbaum) feed and larvae; (2) the evaluation of their genetic relatedness; (3) the study of their antimicrobial/bacteriocin activity against fish pathogens; and (4) the biochemical and genetic characterization of the bacteriocin produced by the strain displaying the greatest antimicrobial activity. Concerning the safety assessment, none of the pediococci showed antibiotic resistance nor produced hemolysin or gelatinase, degraded gastric mucin, or deconjugated bile salts. Four strains (50%) produced tyramine or putrescine, but the corresponding genes were not amplified by PCR. Enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) fingerprinting allowed clustering of the pediococci into 2 well-defined groups (68% similarity). From the 8 pediococci displaying direct antimicrobial activity against at least 3 out of 9 fish pathogens, 6 strains (75%) were identified as bacteriocin producers. The bacteriocin produced by P. acidilactici L-14 was purified, and mass spectrometry and DNA sequencing revealed its identity to pediocin PA-1 (PedPA-1). Altogether, our results allowed the identification of 4 (50%) putatively safe pediococci, including 2 bacteriocinogenic strains. ERIC-PCR fingerprinting was a valuable tool for genetic profiling of P. acidilactici strains. This work reports for the first time the characterization of a PedPA-1-producing P. acidilactici strain isolated from an aquatic environment (rainbow trout larvae), which shows interesting properties related to its potential use as a probiotic in aquaculture.
dc.description.departmentDepto. de Nutrición y Ciencia de los Alimentos
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economia y Competitividad
dc.description.sponsorshipComunidad de Madrid
dc.description.sponsorshipUniversidad Complutense de Madrid
dc.description.sponsorshipFundação para a Ciência e a Tecnologia (FCT, Portugal)
dc.description.sponsorshipEuropean Social Fund
dc.description.statuspub
dc.identifier.citationAraújo C, Muñoz-Atienza E, Poeta P, Igrejas G, Hernández PE, Herranz C, Cintas LM (2016) Characterization of Pediococcus acidilactici strains isolated from rainbow trout (Oncorhynchus mykiss) feed and larvae: safety, DNA fingerprinting, and bacteriocinogenicity. Dis Aquat Org 119:129-143. https://doi.org/10.3354/dao02992
dc.identifier.doi10.3354/dao02992
dc.identifier.essn1616-1580
dc.identifier.issn0177-5103
dc.identifier.officialurlhttps://www.int-res.com/journals/dao/dao-home/
dc.identifier.pmid27137071
dc.identifier.urihttps://hdl.handle.net/20.500.14352/95012
dc.issue.number2
dc.journal.titleDiseases of Aquatic Organisms
dc.language.isoeng
dc.page.final143
dc.page.initial129
dc.relation.projectIDAGL2012-34829
dc.relation.projectIDS2009/AGR-1489
dc.relation.projectIDP2013/ABI-2747
dc.relation.projectIDSFRH/BD/62416/ 2009
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu61
dc.subject.keywordAquaculture
dc.subject.keywordOncorhynchus mykiss
dc.subject.keywordLactic acid bacteria
dc.subject.keywordAnti-fish pathogen activity
dc.subject.keywordFish probiotics
dc.subject.keywordERIC-PCR
dc.subject.ucmCiencias Biomédicas
dc.subject.unesco32 Ciencias Médicas
dc.subject.unesco24 Ciencias de la Vida
dc.titleCharacterization of Pediococcus acidilactici strains isolated from rainbow trout (Oncorhynchus mykiss, Walbaum) feed and larvae: safety, DNA fingerprinting and bacteriocinogenicity
dc.typejournal article
dc.type.hasVersionAM
dc.volume.number119
dspace.entity.typePublication
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relation.isAuthorOfPublication.latestForDiscovery22d49290-f994-42ad-b8ce-bfe1f9c114c8
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