Extreme metal adapted, knockout and knockdown strains reveal a coordinated gene expression among different Tetrahymena thermophila metallothionein isoforms

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dc.contributor.authorFrancisco Martínez, Patricia de
dc.contributor.authorMartín González, Ana María
dc.contributor.authorTurkewitz, Aaron P.
dc.contributor.authorGutiérrez Fernández, Juan Carlos
dc.date.accessioned2023-06-17T22:22:48Z
dc.date.available2023-06-17T22:22:48Z
dc.date.issued2017
dc.description.abstractMetallothioneins (MT) constitute a superfamily of small cytosolic proteins that are able to bind metal cations through numerous cysteine (Cys) residues. Like other organisms the ciliate Tetrahymena thermophila presents several MT isoforms, which have been classified into two subfamilies (Cd- and Cu-metallothioneins). The main aim of this study was to examine the specific functions and transcriptional regulation of the five MT isoforms present in T. thermophila, by using several strains of this ciliate. After a laboratory evolution experiment over more than two years, three different T. thermophila strains adapted to extreme metal stress (Cd2+, Cu2+ or Pb2+) were obtained. In addition, three knockout and/or knockdown strains for different metallothionein (MT) genes were generated. These strains were then analyzed for expression of the individual MT isoforms. Our results provide a strong basis for assigning differential roles to the set of MT isoforms. MTT1 appears to have a key role in adaptation to Cd. In contrast, MTT2/4 are crucial for Cu-adaptation and MTT5 appears to be important for Pb-adaptation and might be considered as an “alarm” MT gene for responding to metal stress. Moreover, results indicate that likely a coordinated transcriptional regulation exists between the MT genes, particularly among MTT1, MTT5 and MTT2/4. MTT5 appears to be an essential gene, a first such report in any organism of an essential MT gene.
dc.description.departmentDepto. de Genética, Fisiología y Microbiología
dc.description.facultyFac. de Ciencias Biológicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía y Competitividad (MINECO)
dc.description.sponsorshipNIH
dc.description.sponsorshipMinisterio de Educación, Cultura y Deporte (MECD)
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/46739
dc.identifier.doi10.1371/journal.pone.0189076
dc.identifier.issn1932-6203
dc.identifier.officialurlhttp://journals.plos.org/plosone/
dc.identifier.urihttps://hdl.handle.net/20.500.14352/18439
dc.journal.titlePLoS ONE
dc.language.isoeng
dc.publisherPLOS
dc.relation.projectIDCGL2016-75494-R
dc.relation.projectIDGM-105783
dc.relation.projectIDFPU12/02789
dc.rightsAtribución 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/es/
dc.subject.cdu579
dc.subject.keywordcytosolic proteins
dc.subject.keywordTetrahymena thermophila
dc.subject.keywordMT genes
dc.subject.ucmBiología
dc.subject.ucmMicrobiología (Biología)
dc.subject.unesco24 Ciencias de la Vida
dc.subject.unesco2414 Microbiología
dc.titleExtreme metal adapted, knockout and knockdown strains reveal a coordinated gene expression among different Tetrahymena thermophila metallothionein isoforms
dc.typejournal article
dspace.entity.typePublication
relation.isAuthorOfPublicatione48abb18-103b-4edc-9081-81a44bc92b44
relation.isAuthorOfPublication0942b309-8e93-4ae5-a284-d49cb191c64b
relation.isAuthorOfPublication.latestForDiscoverye48abb18-103b-4edc-9081-81a44bc92b44
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