Para depositar en Docta Complutense, identifícate con tu correo @ucm.es en el SSO institucional. Haz clic en el desplegable de INICIO DE SESIÓN situado en la parte superior derecha de la pantalla. Introduce tu correo electrónico y tu contraseña de la UCM y haz clic en el botón MI CUENTA UCM, no autenticación con contraseña.

Intradermal Tuberculin Test in Water Buffalo (Bubalus bubalis): Experimental use of Mycobacterial Antigens for the Diagnosis of Bovine Tuberculosis

dc.contributor.authorMartucciello, Alessandra
dc.contributor.authorMazzone, Piera
dc.contributor.authorNapolitano, Francesco
dc.contributor.authorBezos Garrido, Javier
dc.contributor.authorGrandoni, Francesco
dc.contributor.authorBoniotti, Maria Beatrice
dc.contributor.authorCagiola, Monica
dc.contributor.authorCappelli, Giovanna
dc.contributor.authorDi Vuolo, Gabriele
dc.contributor.authorGaliero, Giorgio
dc.contributor.authorSignorelli, Federica
dc.contributor.authorDe Carlo, Esterina
dc.date.accessioned2024-04-23T13:15:31Z
dc.date.available2024-04-23T13:15:31Z
dc.date.issued2024-02-28
dc.description.abstractThe study aims to evaluate the potential use of mycobacterial ESAT6 and CFP10 antigens, Early Secretory Proteins (ESP) in the Skin Test used for bovine tuberculosis (TB) diagnosis in Water Buffalo. A pilot study was performed on 21 buffaloes from a TB outbreak and 11 buffaloes from a TB-free herd. Three concentrations of ESAT6-CFP10 (10, 20, and 30 mg) and two of ESP (50 and 100 µg) were inoculated in the Skin Test, along with PPDB, PPDA, and PBS as a negative control. Skin thickness was measured with calipers before the test and every 24 hours for 4 days. Then, to evaluate the specificity of the antigens, a field study was conducted, and 100 buffaloes from a TB-free herd were inoculated using the best antigens concentration derived from the pilot study. In the positive buffaloes, the strongest skin response was to PPDB at 24h, with some subjects becoming inconclusive at 72 and 96 h. A peak response to PPDA at 48 hours was detected, followed by a slight decrease. The response to ESP-100 µg remained high at 24 and 48 h, then decreased, remaining positive at 72 h. In the 100 TB-free buffaloes, the best specificity was observed using ESAT6-CFP10 and ESP. ESP yielded the best results, showing higher reactivity in infected animals and no reactivity in the healthy ones at 72 h. Therefore, ESP could be an excellent candidate for further extensive studies in the buffalo species to improve Skin Test performance.
dc.description.departmentDepto. de Sanidad Animal
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipMinistry of Health (Italia)
dc.description.sponsorshipEuropean Commission
dc.description.statuspub
dc.identifier.citationMartucciello, A. ., Mazzone, P. ., Napolitano, F. ., Bezos, J. ., Grandoni, F. ., Boniotti, M. B. ., Cagiola, M. ., Cappelli, G. ., Di Vuolo, G. ., Galiero, G. ., Signorelli, F. ., & De Carlo, E. . (2024). Intradermal Tuberculin Test in Water Buffalo (Bubalus bubalis): Experimental use of Mycobacterial Antigens for the Diagnosis of Bovine Tuberculosis. Journal of Buffalo Science, 13, 46–52. https://doi.org/10.6000/1927-520X.2024.13.05
dc.identifier.doi10.6000/1927-520X.2024.13.05
dc.identifier.essn1927-520X
dc.identifier.issn1927-5196
dc.identifier.officialurlhttps://doi.org/10.6000/1927-520X.2024.13.05
dc.identifier.urihttps://hdl.handle.net/20.500.14352/103383
dc.journal.titleJournal of Buffalo Science
dc.language.isoeng
dc.page.final52
dc.page.initial46
dc.publisherLifescience Global
dc.relation.projectIDinfo:eu-repo/grantAgreement/ItalianMinistryOfHealth/IZSME/009/2019
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/862605
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu636.09
dc.subject.keywordWater buffalo
dc.subject.keywordTuberculosis
dc.subject.keywordDiagnosis
dc.subject.keywordTuberculin skin test
dc.subject.keywordESAT6-CFP10
dc.subject.keywordMycobacterium bovis antigens
dc.subject.ucmVeterinaria
dc.subject.unesco3109 Ciencias Veterinarias
dc.titleIntradermal Tuberculin Test in Water Buffalo (Bubalus bubalis): Experimental use of Mycobacterial Antigens for the Diagnosis of Bovine Tuberculosis
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number13
dspace.entity.typePublication
relation.isAuthorOfPublication2e80b9e6-3809-4756-9ef8-ebc9ce005eb8
relation.isAuthorOfPublication.latestForDiscovery2e80b9e6-3809-4756-9ef8-ebc9ce005eb8

Download

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
JBSV13A5-Mazzone.pdf
Size:
621.53 KB
Format:
Adobe Portable Document Format
Description:
Intradermal Tuberculin Test in Water Buffalo (Bubalus bubalis): Experimental use of Mycobacterial Antigens for the Diagnosis of Bovine Tuberculosis

Collections