Angiogenesis inhibitor or aggressiveness marker? The function of endostatin in cancer through electrochemical biosensing

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dc.contributor.authorTejerina Miranda, Sandra
dc.contributor.authorPedrero Muñoz, María
dc.contributor.authorBlázquez García, Marina
dc.contributor.authorSerafín González-Carrato, Verónica
dc.contributor.authorMontero Calle, Ana
dc.contributor.authorGarranzo Asensio, María
dc.contributor.authorReviejo García, Ángel Julio
dc.contributor.authorPingarrón Carrazón, José Manuel
dc.contributor.authorBarderas Manchado, Rodrigo
dc.contributor.authorCampuzano Ruiz, Susana
dc.date.accessioned2024-07-04T13:33:30Z
dc.date.available2024-07-04T13:33:30Z
dc.date.issued2024-02
dc.description.abstractThis work reports the first electrochemical bioplatform developed for the determination of human endostatin (HE), a biomarker with recognized antiangiogenic potential whose elevated circulating levels have also been associated with the development of aggressive cancers. The developed electroanalytical biotool combines the benefits of using magnetic microparticles for the implementation of sandwich immunoassays and amperometric transduction on disposable carbon electrodes. A limit of detection (LOD) of 34.1 pg mL−1 for HE standards and a selectivity suitable for its foray into the clinical oncology area, are demonstrated. The determination of HE in clinical samples such as lysates and secretomes of colorectal cancer (CRC) cells, plasma, and tissue samples from patients with CRC in different stages, has been faced with satisfactory results showing the ability for discriminating the metastatic capabilities of cells and for identifying and staging CRC patients. The developed bioplatform allows precise quantitative determinations, requiring minimal pre-treatments and sample amounts in only 75 min. In addition, due to the instrumentation and the type of substrates used in the detection step, the biotool is compatible with implementation in multiplexed and/or point-of-need devices, features in which this bioplatform is advantageous with respect to the enzyme linked immunosorbent assay (ELISA) or immunoblotting technologies.
dc.description.departmentDepto. de Química Analítica
dc.description.facultyFac. de Ciencias Químicas
dc.description.fundingtypeDescuento UCM
dc.description.refereedTRUE
dc.description.statuspub
dc.identifier.citationandra Tejerina-Miranda, María Pedrero, Marina Blázquez-García, Verónica Serafín, Ana Montero-Calle, Maria Garranzo-Asensio, A. Julio Reviejo, José M. Pingarrón, Rodrigo Barderas, Susana Campuzano, Angiogenesis inhibitor or aggressiveness marker? The function of endostatin in cancer through electrochemical biosensing, Bioelectrochemistry, Volume 155, 2024, 108571, ISSN 1567-5394, https://doi.org/10.1016/j.bioelechem.2023.108571.
dc.identifier.doi10.1016/j.bioelechem.2023.108571
dc.identifier.issn1567-5394
dc.identifier.officialurlhttps://doi.org/10.1016/j.bioelechem.2023.108571
dc.identifier.relatedurlhttps://www.sciencedirect.com/science/article/pii/S1567539423002086?via%3Dihub
dc.identifier.urihttps://hdl.handle.net/20.500.14352/105630
dc.journal.titleBioelectrochemistry
dc.language.isoeng
dc.publisherElsevier
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.cdu543
dc.subject.keywordElectrochemical bioplatform
dc.subject.keywordEndostatin
dc.subject.keywordAngiogenesis
dc.subject.keywordColorectal cancer aggressiveness
dc.subject.keywordTissue
dc.subject.keywordPlasma
dc.subject.ucmCiencias
dc.subject.unesco2301 Química Analítica
dc.titleAngiogenesis inhibitor or aggressiveness marker? The function of endostatin in cancer through electrochemical biosensing
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number155
dspace.entity.typePublication
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