Epidermal Growth Factor Impairs Palatal Shelf Adhesion and Fusion in the T gf-β3 Null Mutant

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dc.contributor.authorBarrio Asensio, María Del Carmen
dc.contributor.authorRío Sevilla, Aurora Del
dc.contributor.authorMurillo González, Jorge Alfonso
dc.contributor.authorMaldonado Bautista, Estela
dc.contributor.authorLópez Gordillo, Yamila
dc.contributor.authorParadas Lara, Irene
dc.contributor.authorHernandes, Luzmarina
dc.contributor.authorCatón Vázquez, Javier
dc.contributor.authorMartínez Álvarez, Concepción
dc.date.accessioned2023-06-19T15:03:03Z
dc.date.available2023-06-19T15:03:03Z
dc.date.copyrightAccepted after revision: March 14, 2014 Published online: May 23, 2014
dc.date.issued2014
dc.description.abstractThe cleft palate presented by transforming growth factor-β3 (Tgf-β3 ) null mutant mice is caused by altered palatal shelf adhesion, cell proliferation, epithelial-to-mesenchymal transformation and cell death. The expression of epidermal growth factor (EGF), transforming growth factor-β1 ( Tgf-β1 ) and muscle segment homeobox-1 (Msx-1) is modified in the palates of these knockout mice, and the cell proliferation defect is caused by the change in EGF expression. In this study, we aimed to determine whether this change in EGF expression has any effect on the other mechanisms altered in Tgf-β 3 knockout mouse palates. We tested the effect of inhibiting EGF activity in vitro in the knockout palates via the addition of Tyrphostin AG 1478. We also investigated possible interactions between EGF, Tgf-β 1 and Msx-1 in Tgf-β 3 null mouse palate cultures. The results show that the inhibition of EGF activity in Tgf-β 3 null mouse palate cultures improves palatal shelf adhesion and fusion, with a particular effect on cell death, and restores the normal distribution pattern of Msx-1 in the palatal esenchyme. Inhibition of TGF-β 1 does not affect either EGF or Msx-1 expression.en
dc.description.departmentDepto. de Anatomía y Embriología
dc.description.departmentUnidad Docente de Anatomía y Embriología
dc.description.facultyFac. de Medicina
dc.description.facultyFac. de Óptica y Optometría
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía, Comercio y Empresa (España)
dc.description.sponsorshipUniversidad Complutense
dc.description.sponsorshipComunidad Autónoma de Madrid
dc.description.sponsorshipBanco Santander
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/38561
dc.identifier.citationBarrio Asensio, M. C., Rio Sevilla, A., Murillo González, J. A. et al. «Epidermal Growth Factor Impairs Palatal Shelf Adhesion and Fusion in the Tgf-β3 Null Mutant». Cells Tissues Organs, vol. 199, n.o 2-3, mayo de 2014, pp. 201-11. Silverchair, https://doi.org/10.1159/000362227.
dc.identifier.doi10.1159/000362227
dc.identifier.issn1422-6405
dc.identifier.officialurlhttp://dx.doi.org/10.1159/000362227
dc.identifier.relatedurlhttp://www.karger.com/Article/FullText/362227
dc.identifier.urihttps://hdl.handle.net/20.500.14352/35215
dc.issue.number2-3
dc.journal.titleCells Tissues Organs
dc.language.isoeng
dc.page.final2011
dc.page.initial201
dc.publisherKarger
dc.relation.projectIDPI06/0184
dc.relation.projectIDPS09/01762
dc.relation.projectIDCCG07-ECM/BIO-2592
dc.rights.accessRightsrestricted access
dc.subject.cdu612.315
dc.subject.cdu611.315
dc.subject.cdu616.314
dc.subject.keywordTgf-beta3
dc.subject.keywordTgf-beta1
dc.subject.keywordEpidermal growth factor
dc.subject.keywordMsx-1
dc.subject.keywordKnockout
dc.subject.keywordPalate development
dc.subject.ucmMedicina
dc.subject.ucmFisiología
dc.subject.ucmNeurociencias (Medicina)
dc.subject.ucmOdontología (Medicina)
dc.subject.unesco32 Ciencias Médicas
dc.subject.unesco2411 Fisiología Humana
dc.subject.unesco2490 Neurociencias
dc.titleEpidermal Growth Factor Impairs Palatal Shelf Adhesion and Fusion in the T gf-β3 Null Mutant
dc.typejournal article
dc.volume.number199
dspace.entity.typePublication
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relation.isAuthorOfPublication.latestForDiscovery7a351e41-71e3-4b0e-b130-49213b6b3d50
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