In vitro and in vivo genotoxicity assessment of gold nanoparticles of different sizes by comet and SMART assays

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dc.contributor.authorÁvalos, A.
dc.contributor.authorHaza Duaso, Ana Isabel
dc.contributor.authorMateo, D.
dc.contributor.authorMorales Gómez, Paloma
dc.date.accessioned2024-01-16T15:47:58Z
dc.date.available2024-01-16T15:47:58Z
dc.date.issued2018-06-28
dc.description.abstractDue to the increasing use of gold nanoparticles (AuNPs) in different areas such as medicine, biotechnology or food sector, human exposure to them has grown significantly and its toxicity evaluation has become essential. Therefore, the purpose of this study was to compare the potential genotoxic effects of 30, 50 and 90 nm AuNPs, using in vitro comet assay with the in vivo mutagenic and recombinogenic activity (SMART Test) in Drosophila. The results indicated that in both cell lines, 30, 50 and 90 nm (1–10 μg ml−1) AuNPs increased DNA strand breaks following 24 h treatment. This damage was not dose and size-dependent. Moreover, a modified comet assay using endonuclease III and formamidopyrimidine-DNA glycosylase restriction enzymes showed that in both cell lines, pyrimidines and purines were oxidatively damaged by all AuNPs, being 90 nm AuNPs slightly more genotoxic. However, the data obtained with SMART showed that 30 nm AuNPs did not modify the spontaneous frequencies of spots indicating lack of mutagenic and recombinogenic activity. Therefore, further experiments must be carried out to gain a better understanding of the mechanism of action of AuNPs to ensure their safe use.
dc.description.departmentSección Dptal. de Nutrición y Ciencia de los Alimentos (Veterinaria)
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia, Innovación y Universidades - Grant/Award Number: PGC2018-096198-A-I00
dc.description.statuspub
dc.identifier.citationÁvalos, A., Haza, A.I. y Morales, P. In vitro and in vivo genotoxicity of gold nanoparticles by comet and SMART assays. Food and Chemical Toxicology, 2018, 120: 81-88. https://doi.org/10.1016/j.fct.2018.06.061.
dc.identifier.doi10.1016/j.fct.2018.06.061
dc.identifier.issn0278-6915
dc.identifier.officialurlhttps://www.sciencedirect.com/science/article/pii/S0278691518304356?via%3Dihub
dc.identifier.urihttps://hdl.handle.net/20.500.14352/93421
dc.journal.titleFood and Chemical Toxicology
dc.language.isoeng
dc.page.final88
dc.page.initial81
dc.publisherElsevier
dc.relation.projectIDAGL2010-16561
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-096198-A-I00/ES/APLICACION GENOMICA Y BUSQUEDA DE INDICADORES DE RESILIENCIA EN UN EXPERIMENTO DE SELECCION DIVERGENTE PARA LA VARIABILIDAD AMBIENTAL DEL PESO AL NACIMIENTO EN MUS MUSCULUS/
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.cdu663/665
dc.subject.keywordComet assay
dc.subject.keywordDrosophila
dc.subject.keywordGenotoxicity
dc.subject.keywordHuman cell lines
dc.subject.keywordGold nanoparticles
dc.subject.keywordSMART
dc.subject.ucmTecnología de los alimentos
dc.subject.unesco3309 Tecnología de Los Alimentos
dc.subject.unesco3212 Salud Publica
dc.titleIn vitro and in vivo genotoxicity assessment of gold nanoparticles of different sizes by comet and SMART assays
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number120
dspace.entity.typePublication
relation.isAuthorOfPublication7b71c224-30ca-42fc-be0f-98647103a21f
relation.isAuthorOfPublication80805744-56b4-475b-bb25-b3be6622b96c
relation.isAuthorOfPublication.latestForDiscovery80805744-56b4-475b-bb25-b3be6622b96c
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