Electrochemical biosensor for creatinine based on the immobilization of creatininase, creatinase and sarcosine oxidase onto a ferrocene/horseradish peroxidase/gold nanoparticles/multi-walled carbon nanotubes/Teflon composite electrode

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dc.contributor.authorSerafín González-Carrato, Verónica
dc.contributor.authorHernández, Paloma
dc.contributor.authorAgüí Chicharro, María Lourdes
dc.contributor.authorYáñez-Sedeño Orive, Paloma
dc.contributor.authorPingarrón Carrazón, José Manuel
dc.date.accessioned2024-01-22T17:11:39Z
dc.date.available2024-01-22T17:11:39Z
dc.date.issued2013
dc.description.abstractA composite electrode consisted of gold nanoparticles (AuNPs), multi-walled carbon nanotubes (MWCNTs) and Teflon, to which peroxidase (HRP) and ferrocene (Fc) were incorporated as auxiliary enzyme and redox mediator, respectively, was constructed. The enzymes creatininase, creatinase and sarcosine oxidase were then co-immobilized onto the surface of the resulting HRP/Fc/AuNPs/MWCNTs/Teflon electrode for the preparation of a creatinine biosensor. Amperometry in stirred solutions using a detection potential of 0.0 V vs Ag/AgCl allowed a linear calibration plot to be obtained in the 0.003–1.0 mM creatinine concentration range with a limit of detection of 0.1 M (S/N = 3). The apparent Michaelis-Menten constant for creatininase was KMap= 4.1 ± 0.4 mM. The developed biosensor was validated with good results by determining creatinine in human serum and correlating with the spectrophotometric Jaffe’s method.
dc.description.departmentDepto. de Química Analítica
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia e Innovación (España)
dc.description.sponsorshipComunidad de Madrid
dc.description.statuspub
dc.identifier.citationSerafín V, Hernández P, Agüí L, Yánez-Sedeño P, Pingarrón J.M. Electrochemical biosensor for creatinine based on the immobilization of creatininase, creatinase and sarcosine oxidase onto a ferrocene/horseradish peroxidase/gold nanoparticles/multi-walled carbon nanotubes/Teflon composite electrode. Electrochim. Acta. 2013 Marzo 13; 97: 175-83
dc.identifier.doi10.1016/j.electacta.2013.03.005
dc.identifier.issn0013-4686
dc.identifier.officialurlhttps://doi.org/10.1016/j.electacta.2013.03.005
dc.identifier.relatedurlhttps://produccioncientifica.ucm.es/documentos/5d9daf07299952484ef814c8
dc.identifier.urihttps://hdl.handle.net/20.500.14352/94510
dc.journal.titleElectrochimica Acta
dc.language.isoeng
dc.page.final183
dc.page.initial175
dc.publisherElsevier Ltd
dc.relation.projectIDCTQ2012-35041
dc.relation.projectIDS2009/PPQ1642
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsrestricted access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.cdu544.6:543.25
dc.subject.keywordCreatinine
dc.subject.keywordElectrochemical biosensor
dc.subject.keywordGold nanoparticles
dc.subject.keywordCarbon nanotubes
dc.subject.keywordComposite electrodes
dc.subject.ucmQuímica analítica (Química)
dc.subject.unesco2301 Química Analítica
dc.titleElectrochemical biosensor for creatinine based on the immobilization of creatininase, creatinase and sarcosine oxidase onto a ferrocene/horseradish peroxidase/gold nanoparticles/multi-walled carbon nanotubes/Teflon composite electrode
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number97
dspace.entity.typePublication
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