Comparación del microbiona subgingival entre salud/gingivitis y periodontitis estadio III/IV.

Thumbnail Image
Official URL
Full text at PDC
Publication Date
Advisors (or tutors)
Journal Title
Journal ISSN
Volume Title
Google Scholar
Research Projects
Organizational Units
Journal Issue
El objetivo principal de este trabajo, es comparar las diferencias a nivel de microbioma entre sujetos españoles con un estado periodontal de salud/gingivitis y diagnosticados con periodontitis estadio III/IV, utilizando para ello el sistema de secuenciación MiSeq (Illumina®, San Diego/California, USA).
Trabajo Fin de Máster encuadrado en la línea de investigación: Eficacia de intervenciones para el tratamiento de enfermedades periodontales.
UCM subjects
1. Chapple ILC, Mealey BL, Van Dyke TE, Bartold PM, Dommisch H, Eickholz P, et al. Periodontal health and gingival diseases and conditions on an intact and a reduced periodontium: Consensus report of workgroup 1 of the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions. J Clin Periodontol. 2018;45 Suppl 20:S68-S77. 2. Papapanou PN, Sanz M, Buduneli N, Dietrich T, Feres M, Fine DH, et al. Periodontitis: Consensus report of workgroup 2 of the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions. J Clin Periodontol. 2018;45 Suppl 20:S162‐S170. 3. Heaton B, Dietrich T. Causal theory and the etiology of periodontal diseases. Periodontol 2000. 2012;58(1):26‐36. 4. The pathogenesis of periodontal diseases. J Periodontol. 1999;70(4):457‐470. 5. Hajishengallis G. Immunomicrobial pathogenesis of periodontitis: keystones,pathobionts, and host response. Trends Immunol. 2014;35(1):3‐11. 6. Darveau RP. Periodontitis: a polymicrobial disruption of host homeostasis. Nat Rev Microbiol. 2010;8(7):481‐490. 7. Chimenos-Küstner E, Giovannoni ML, Schemel-Suárez M. Dysbiosis as a determinant factor of systemic and oral pathology: importance of microbiome. Med Clin (Barc). 2017;149(7):305‐309. 8. Lederberg J, McCray AT. 'Ome Sweet 'Omics - A genealogical treasury of words genealogical treasury of words. The Scientist. 2001;15(7):8. 9. Baquero F, Nombela C. The microbiome as a human organ. Clin Microbiol Infect. 2012;18 Suppl 4:2‐4. 10. Kilian M, Chapple IL, Hannig M, Marsh PD, Meuric V, Pedersen AML, et al. The oral microbiome - an update for oral healthcare professionals. Br Dent J. 2016;221(10):657‐666. 11. Bartold PM, Van Dyke TE. An appraisal of the role of specific bacteria in the initial pathogenesis of periodontitis. J Clin Periodontol. 2019;46(1):6‐11. 12. Dongari-Bagtzoglou A. Pathogenesis of mucosal biofilm infections: challenges and progress. Expert Rev Anti Infect Ther. 2008;6(2):201‐208. 13. Kolenbrander PE, Palmer RJ Jr, Periasamy S, Jakubovics NS. Oral multispecies biofilm development and the key role of cell-cell distance. Nat Rev Microbiol. 2010;8(7):471‐480. 14. Wade WG. Has the use of molecular methods for the characterization of the human oral microbiome changed our understanding of the role of bacteria in the pathogenesis of periodontal disease?. J Clin Periodontol. 2011;38 Suppl 11:7‐16. 15. Ronaghi M. Pyrosequencing sheds light on DNA sequencing. Genome Res.2001;11(1):3‐11. 16. Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977;74(12):5463‐5467. 17. Slatko BE, Gardner AF, Ausubel FM. Overview of next-generation sequencing technologies. Curr Protoc Mol Biol. 2018;122(1):e59. 18. Morgan XC, Huttenhower C. Chapter 12: Human microbiome analysis. PLoS Comput Biol.012;8(12):e1002808. 19. Hamady M, Knight R. Microbial community profiling for human microbiome projects: Tools, techniques, and challenges. Genome Res. 2009;19(7):1141‐1152. 20. Lozupone CA, Knight R. Species divergence and the measurement of microbial diversity. FEMS Microbiol Rev. 2008;32(4):557‐578. 21. Chao A. Nonparametric estimation of the number of classes in a population. Scand Stat Theory Appl. 1984; 11(4):265-270. 22. Chao A, Ma MC, Yang MCK. Stopping rules and estimation for recapture debugging with unequal failure rates. Biometrika. 1993;80(1):193-201. 23. Heltshe JF, Forrester NE. Estimating species richness using the jackknife procedure. Biometrics. 1983;39(1):1‐11. 24. Colwell RK, Coddington JA. Estimating terrestrial biodiversity through extrapolation. Philos Trans R Soc Lond B Biol Sci. 1994;345(1311):101‐118. 25. Shannon CE. A mathematical theory of communication. Bell Syst Tech J. 1948;27(3):379-423. 26. Simpson EH. Measurement of diversity. Nature. 1949;163(4148):688. 27. Bray JR, Curtis JT. An ordination of the upland forest communities of Southern Wisconsin. Ecol Monogr. 1957;27(4):325-349. 28. Wade WG. The oral microbiome in health and disease. Pharmacol Res. 2013;69(1):137‐143. 29. Krishnan K, Chen T, Paster BJ. A practical guide to the oral microbiome and its relation to health and disease. Oral Dis. 2017;23(3):276‐286. 30. Dewhirst FE, Chen T, Izard J, Paster BJ, Tanner ACR, Yu WH, et al. The human oral microbiome. J Bacteriol. 2010;192(19):5002‐5017 31. Donlan RM, Costerton JW. Biofilms: survival mechanisms of clinically relevant microorganisms. Clin Microbiol Rev. 2002;15(2):167‐193. 32. Verma D, Garg PK, Dubey AK. Insights into the human oral microbiome. Arch Microbiol. 2018;200(4):525‐540. 33. Mason MR, Preshaw PM, Nagaraja HN, Dabdoub SM, Rahman A, Kumar PS. The subgingival microbiome of clinically healthy current and never smokers. ISME J. 2015;9(1):268‐272. 34. Li J, Quinque D, Horz HP, Li M, Rzhetskaya M, Raff JA, et al. Comparative analysis of the human saliva microbiome from different climate zones: Alaska, Germany, and Africa. BMC Microbiol. 2014;14:316. 35. Segata N, Haake SK, Mannon P, Lemon KP, Waldron L, Gevers D, et al.Composition of the adult digestive tract bacterial microbiome based on seven mouth surfaces, tonsils, throat and stool samples. Genome Biol. 2012;13(6):R42. 36. Chen H, Jiang W. Application of high-throughput sequencing in understanding human oral microbiome related with health and disease. Front Microbiol. 2014;5:508. 37. Bik EM, Long CD, Armitage GC, Loomer P, Emerson J, Mongodin EF, et al. Bacterial diversity in the oral cavity of 10 healthy individuals. ISME J. 2010;4(8):962‐974. 38. Aas JA, Paster BJ, Stokes LN, Olsen I, Dewhirst FE. Defining the normal bacterial flora of the oral cavity. J Clin Microbiol. 2005;43(11):5721‐5732. 39. Consensus report. Periodontal diseases: pathogenesis and microbial factors. Ann Periodontol. 1996;1(1):926‐932. 40. Hajishengallis G, Darveau RP, Curtis MA. The keystone-pathogen hypothesis. Nat Rev Microbiol. 2012;10(10):717‐725. 41. O'Leary TJ, Drake RB, Naylor JE. The plaque control record. J Periodontol.1972;43(1):38. 42. Badersten A, Nilvéus R, Egelberg J. Effect of nonsurgical periodontal therapy. VII. Bleeding, suppuration and probing depth in sites with probing attachment loss. J Clin Periodontol. 1985;12(6):432‐440. 43. Syed SA, Loesche WJ. Survival of human dental plaque flora in various transport media. Appl Microbiol. 1972;24(4):638‐644. 44. Schulz S, Porsch M, Grosse I, Hoffmann K, Schaller HG, Reichert S. Comparison of the oral microbiome of patients with generalized aggressive periodontitis and periodontitis-free subjects. Arch Oral Biol. 2019;99:169‐176. 45. Achtman M, Wagner M. Microbial diversity and the genetic nature of microbial species. Nat Rev Microbiol. 2008;6(6):431‐440. 46. Schloss PD. The effects of alignment quality, distance calculation method, sequence filtering, and region on the analysis of 16S rRNA gene-based studies. PLoS Comput Biol. 2010;6(7):e1000844. 47. Shi M, Wei Y, Hu W, Nie Y, Wu X, Lu R. The subgingival microbiome of periodontal pockets with different probing depths in chronic and aggressive periodontitis: A pilot study. Front Cell Infect Microbiol. 2018;8:124. 48. Liu B, Faller LL, Klitgord N, Mazumdar V, Ghodsi M, Sommer DD, et al. Deep sequencing of the oral microbiome reveals signatures of periodontal disease. PLoS One. 2012;7(6):e37919. 49. Deng ZL, Szafrański SP, Jarek M, Bhuju S, Wagner-Döbler I. Dysbiosis in chronic periodontitis: key microbial players and interactions with the human host. Sci Rep.2017;7(1):3703. 50. Socransky SS, Haffajee AD. Periodontal microbial ecology. Periodontol 2000.2005;38:135‐187. 51. Ai D, Huang R, Wen J, Li C, Zhu J, Xia LC. Integrated metagenomic data analysis demonstrates that a loss of diversity in oral microbiota is associated with periodontitis. BMC Genomics. 2017;18(Suppl 1):1041. 52. Galimanas V, Hall MW, Singh N, Lynch MDJ, Goldberg M, Tenenbaum H, et al.Bacterial community composition of chronic periodontitis and novel oral sampling sites for detecting disease indicators. Microbiome. 2014;2:32. 53. Abusleme L, Dupuy AK, Dutzan N, Silva N, Burleson JA, Strausbaugh LD, et al. The subgingival microbiome in health and periodontitis and its relationship with community biomass and inflammation. ISME J. 2013;7(5):1016‐1025. 54. Griffen AL, Beall CJ, Campbell JH, Firestone ND, Kumar PS, Yang ZK, et al. Distinct and complex bacterial profiles in human periodontitis and health revealed by 16S pyrosequencing. ISME J. 2012;6(6):1176‐1185. 55. Duran-Pinedo AE, Paster B, Teles R, Frias-Lopez J. Correlation network analysis applied to complex biofilm communities. PLoS One. 2011;6(12):e28438. 56. Duran-Pinedo AE, Frias-Lopez J. Beyond microbial community composition: functional activities of the oral microbiome in health and disease. Microbes Infect. 2015;17(7):505‐516. 57. Yamada R, Okada D, Wang J, Basak T, Koyama S. Interpretation of omics data analyses. J Hum Genet. 2020;10.1038/s10038-020-0763-5. 58. Jiang D, Armour CR, Hu C, Mei M, Tian C, Sharpton TJ, et al. Microbiome multiomics network analysis: tatistical considerations, limitations, and opportunities. Front Genet. 2019;10:995.