Click chemistry-assisted antibodies immobilization for immunosensing of CXCL7 chemokine in serum

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dc.contributor.authorGuerrero Irigoyen, Sara
dc.contributor.authorCadanno Mendía, Dona
dc.contributor.authorAgüí Chicharro, María Lourdes
dc.contributor.authorBarderas Manchado, Rodrigo
dc.contributor.authorCampuzano Ruiz, Susana
dc.contributor.authorYáñez-Sedeño Orive, Paloma
dc.contributor.authorPingarrón Carrazón, José Manuel
dc.date.accessioned2024-01-12T11:36:10Z
dc.date.available2024-01-12T11:36:10Z
dc.date.issued2019
dc.description.abstractThe first electrochemical immunosensor for the determination of CXCL7 (chemokine (C-X-C motif) ligand 7) autoimmune biomarker is reported in this work. Click chemistry-assisted antibodies immobilization was per formed by reaction of azide functionalized-multi-walled carbon nanotubes (MWCNTs) and ethynyl-IgG onto screen-printed carbon electrodes. The capture antibodies were further immobilized onto IgG-MWCNTs con jugates. After a blocking step with casein, a sandwich immunoassay was implemented involving biotinylated detector antibodies and alkaline phosphatase (AP)-streptavidin conjugate. Differential pulse voltammetry upon addition of 1-naphthylphosphate was used as the analytical readout. A linear calibration plot between 0.5 and 600 pg mL−1 CXCL7 and a LOD value of 0.1 pg mL−1 were obtained. The usefulness of the immunosensor was demonstrated by the successful analysis of serum samples from patients with rheumatoid arthritis.en
dc.description.departmentDepto. de Química Analítica
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía, Comercio y Empresa (España)
dc.description.sponsorshipComunidad de Madrid
dc.description.sponsorshipInstituto de Salud Carlos III
dc.description.statuspub
dc.identifier.citationGuerrero S, Cadano D, Agüí L, Barderas R, Campuzano S, Yáñez-Sedeño P, et al. Click chemistry-assisted antibodies immobilization for immunosensing of CXCL7 chemokine in serum. J Electroanal Chem. 2019 Feb 21; 837:246–53.
dc.identifier.doi10.1016/j.jelechem.2019.02.043
dc.identifier.issn1572-6657
dc.identifier.officialurlhttps://doi.org/10.1016/j.jelechem.2019.02.043
dc.identifier.urihttps://hdl.handle.net/20.500.14352/92759
dc.journal.titleJournal of Electroanalytical Chemistry
dc.language.isoeng
dc.page.final253
dc.page.initial246
dc.publisherElsevier
dc.relation.projectIDCTQ2015-70023-R
dc.relation.projectIDCTQ2015-64402-C2-1- R
dc.relation.projectIDTRANSNANOAVANSENS P2018/NMT-4349
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsrestricted access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu543
dc.subject.cdu544.6:543.25
dc.subject.cdu543.25:544.6
dc.subject.keywordChemokines
dc.subject.keywordCXCL7
dc.subject.keywordAutoimmune diseases
dc.subject.keywordRheumatoid arthritis
dc.subject.keywordBiomarkers
dc.subject.keywordClick chemistry
dc.subject.keywordElectrochemical immunosensor
dc.subject.keywordSerum
dc.subject.ucmQuímica analítica (Química)
dc.subject.unesco2301 Química Analítica
dc.subject.unesco2301.04 Análisis Electroquímico
dc.titleClick chemistry-assisted antibodies immobilization for immunosensing of CXCL7 chemokine in serumen
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number837
dspace.entity.typePublication
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