Protective Effect of Thyme and Chestnut Honeys Enriched with Bee Products against Benzo(a)pyrene-Induced DNA Damage

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dc.contributor.authorSánchez Martín, Vanesa
dc.contributor.authorHaza Duaso, Ana Isabel
dc.contributor.authorIriondo De Hond, Amaia
dc.contributor.authorDel Castillo, María Dolores
dc.contributor.authorFernández Hospital, Xavier
dc.contributor.authorFernández Álvarez, Manuela
dc.contributor.authorHierro Paredes, Eva
dc.contributor.authorMorales Gómez, Paloma
dc.contributor.editorTchounwou, Paul B.
dc.date.accessioned2024-01-16T16:06:32Z
dc.date.available2024-01-16T16:06:32Z
dc.date.issued2022-12-17
dc.description.abstractThe aim of the present study was to validate the cytotoxicity, genotoxicity, and preventive potential against benzo(a)pyrene (BaP)-induced DNA damage of nine samples of thyme and chestnut honeys enriched with bee products (royal jelly and propolis, 2–10%). Cell viability was determined by the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay (0–250 mg/mL) to select nontoxic concentrations, and DNA damage (0.1–10 μg/mL) was evaluated by the alkaline single-cell gel electrophoresis or comet assay. Treatment with honey samples or royal jelly and propolis did not affect the viability of HepG2 cells up to 100 and 50 mg/mL, respectively. Treatment with 100 μM BaP significantly increased (p ≤ 0.001) the levels of the DNA strand breaks. None of the tested concentrations (0.1–10 μg/mL) of the honey samples (thyme and chestnut), royal jelly, and propolis caused DNA damage per se. All tested samples at all the concentrations used decreased the genotoxic effect of BaP. In addition, all mixtures of thyme or chestnut honeys with royal jelly or propolis showed a greater protective effect against BaP than the samples alone, being the thyme and chestnut honey samples enriched with 10% royal jelly and 10% propolis the most effective (70.4% and 69.4%, respectively). The observed protective effect may be associated with the phenolic content and antioxidant capacity of the studied samples. In conclusion, the thyme and chestnut honey samples enriched with bee products present potential as natural chemoprotective agents against the chemical carcinogen BaP.
dc.description.departmentSección Dptal. de Nutrición y Ciencia de los Alimentos (Veterinaria)
dc.description.departmentSección Deptal. de Farmacia Galénica y Tecnología Alimentaria (Veterinaria)
dc.description.facultyCAI Ciencias de la Tierra y Arqueometría
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia e Innovación - Grant RTI2018-097549-B-I00
dc.description.statuspub
dc.identifier.citationSánchez-Martín, V.; Haza, A.I.; Iriondo-DeHond, A.; del Castillo, M.D.; Hospital, X.F.; Fernández, M.; Hierro, E.; Morales, P. Protective Effect of Thyme and Chestnut Honeys Enriched with Bee Products against Benzo(a)pyreneInduced DNA Damage. Int. J. Environ. Res. Public Health 2022, 19, 16969. https://doi.org/10.3390/ ijerph192416969
dc.identifier.doi10.3390/ijerph192416969
dc.identifier.issn1660-4601
dc.identifier.officialurlhttps://www.mdpi.com/1660-4601/19/24/16969
dc.identifier.urihttps://hdl.handle.net/20.500.14352/93434
dc.issue.number24
dc.journal.titleInternational Journal of Environmental Research and Public Health
dc.language.isoeng
dc.page.initial16969
dc.publisherMDPI
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-097549-B-I00/ES/ESTRATEGIAS PARA REDUCIR O REEMPLAZAR ADITIVOS ALIMENTARIOS (CONSERVANTES Y ANTIOXIDANTES) EN PRODUCTOS CARNICOS CURADOS/
dc.rights.accessRightsopen access
dc.subject.cdu663/665
dc.subject.keywordAntioxidant
dc.subject.keywordBenzo(a)pyrene
dc.subject.keywordChemopreventive agent
dc.subject.keywordComet assay
dc.subject.keywordDNA damage
dc.subject.keywordGenotoxicity
dc.subject.keywordHoney
dc.subject.keywordRoyal jelly
dc.subject.keywordPropolis
dc.subject.ucmAlimentación
dc.subject.unesco3309 Tecnología de Los Alimentos
dc.titleProtective Effect of Thyme and Chestnut Honeys Enriched with Bee Products against Benzo(a)pyrene-Induced DNA Damage
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number19
dspace.entity.typePublication
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