Enhancing tellurite and selenite bioconversions by overexpressing a methyltransferase from Aromatoleum sp. CIB

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dc.contributor.authorAlonso‐Fernandes, Elena
dc.contributor.authorFernández‐Llamosas, Helga
dc.contributor.authorCano, Irene
dc.contributor.authorSerrano‐Pelejero, Cristina
dc.contributor.authorCastro Ruiz, Laura
dc.contributor.authorDíaz, Eduardo
dc.contributor.authorCarmona, Manuel
dc.date.accessioned2023-12-13T16:20:59Z
dc.date.available2023-12-13T16:20:59Z
dc.date.issued2022
dc.description.abstractPollution by metalloids, e.g., tellurite and selenite, is of serious environmental concern and, therefore, there is an increasing interest in searching for ecologically friendly solutions for their elimination. Some microorganisms are able to reduce toxic tellurite/selenite into less toxic elemental tellurium (Te) and selenium (Se). Here, we describe the use of the environmentally relevant β‐proteobacterium Aromatoleum sp. CIB as a platform for tellurite elimination. Aromatoleum sp. CIB was shown to tolerate 0.2 and 0.5 mM tellurite at aerobic and anaerobic conditions, respectively. Furthermore, the CIB strain was able to reduce tellurite into elemental Te producing rod‐shaped Te nanoparticles (TeNPs) of around 200 nm length. A search in the genome of Aromatoleum sp. CIB revealed the presence of a gene, AzCIB_0135, which encodes a new methyltransferase that methylates tellurite and also selenite. AzCIB_0135 orthologs are widely distributed in bacterial genomes. The overexpression of the AzCIB_0135 gene both in Escherichia coli and Aromatoleum sp. CIB speeds up tellurite and selenite removal, and it enhances the production of rod‐shaped TeNPs and spherical Se nanoparticles (SeNPs), respectively. Thus, the overexpression of a methylase becomes a new genetic strategy to optimize bacterial catalysts for tellurite/selenite bioremediation and for the programmed biosynthesis of metallic nanoparticles of biotechnological interest.
dc.description.departmentDepto. de Ingeniería Química y de Materiales
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia e Innovación (España)
dc.description.statuspub
dc.identifier.citationAlonso-Fernandes, E.; Fernández-Llamosas, H.; Cano, I.; Serrano-Pelejero, C.; Castro, L.; Díaz, E.; Carmona, M. Enhancing tellurite and selenite bioconversions by overexpressing a methyltransferase from Aromatoleum sp. CIB. Microb. Biotechnol. 2023, 16, 915-930.
dc.identifier.doi10.1111/1751-7915.14162
dc.identifier.issn1751-7915
dc.identifier.officialurlhttps://doi.org/10.1111/1751-7915.14162
dc.identifier.urihttps://hdl.handle.net/20.500.14352/91249
dc.journal.titleMicrobial Biotechnology
dc.language.isoeng
dc.page.final930
dc.page.initial915
dc.publisherWiley
dc.relation.projectIDBIO2016-79736-R
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-110612RB-I00/ES/DISEÑO DE NUEVAS ESTRATEGIAS METABOLICAS EN PLATAFORMAS BACTERIANAS MODELO PARA LA BIODETECCION Y BIOCONVERSION DE CONTAMINANTES, Y PARA LA VALORIZACION DE RESIDUOS/
dc.relation.projectIDPCI2019-111833-2
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.cdu66
dc.subject.ucmMetalurgia
dc.subject.unesco3303 Ingeniería y Tecnología Químicas
dc.titleEnhancing tellurite and selenite bioconversions by overexpressing a methyltransferase from Aromatoleum sp. CIB
dc.typejournal article
dc.volume.number16
dspace.entity.typePublication
relation.isAuthorOfPublication93b5b649-29f0-4442-b669-6ee59981ae2b
relation.isAuthorOfPublication.latestForDiscovery93b5b649-29f0-4442-b669-6ee59981ae2b
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