High glucose alters the secretome of mechanically stimulated osteocyte-like cells affecting osteoclast precursor recruitment and differentiation
dc.contributor.author | Maycas, Marta | |
dc.contributor.author | Portolés Pérez, María Teresa | |
dc.contributor.author | Matesanz Sancho, María Concepción | |
dc.contributor.author | Buendía, Irene | |
dc.contributor.author | Linares, Javier | |
dc.contributor.author | Feito Castellano, María José | |
dc.contributor.author | Arcos Navarrete, Daniel | |
dc.contributor.author | Vallet Regí, María Dulce Nombre | |
dc.contributor.author | Plotkin, Lilian I. | |
dc.contributor.author | Esbrit, Pedro | |
dc.contributor.author | Gortázar, Arancha R. | |
dc.date.accessioned | 2023-06-17T22:07:01Z | |
dc.date.available | 2023-06-17T22:07:01Z | |
dc.date.issued | 2017-12 | |
dc.description.abstract | Diabetes mellitus (DM) induces bone deterioration, while mechanical stimulation promotes osteocyte-driven bone formation. We aimed to evaluate the interaction of acute exposure (24 h) to high glucose (HG) with both the pro-survival effect conferred to osteocytic MLO-Y4 cells and osteoblastic MC3T3-E1 cells by mechanical stimulation and the interaction of these cells with osteoclast precursor RAW264.7 cells. We found that 24 h of HG (25 mM) preexposure prevented both cell survival and ERK and β-catenin nuclear translocation upon mechanical stimulation by fluid flow (FF) (10 min) in both MLO-Y4 and MC3T3-E1 cells. However, migration of RAW 264.7 cells was inhibited by MLO-Y4 cell-conditioned medium (CM), but not by MC3T3-E1 cell-CM, with HG or FF. This inhibitory effect was associated with consistent changes in VEGF, RANTES, MIP-1α, MIP-1β MCP-1, and GM-CSF in MLO-Y4 cellCM. RAW264.7 proliferation was inhibited by MLO-Y4 CM under static or HG conditions, but it increased by FF-CM with or without HG. In addition, both FF and HG abrogated the capacity of RAW 264.7 cells to differentiate into osteoclasts, but in a different manner. Thus, HG-CM in static condition allowed formation of osteoclast-like cells, which were unable to resorb hydroxyapatite. In contrast, FF-CM prevented osteoclastogenesis even in HG condition. Moreover, HG did not affect basal RANKL or IL-6 secretion or their inhibition induced by FF in MLO-Y4 cells. In conclusion, this in vitro study demonstrates that HG exerts disparate effects on osteocyte mechanotransduction, and provides a novel mechanism by which DM disturbs skeletal metabolism through altered osteocyte-osteoclast communication. | |
dc.description.department | Sección Deptal. de Bioquímica y Biología Molecular (Biológicas) | |
dc.description.faculty | Fac. de Ciencias Biológicas | |
dc.description.refereed | TRUE | |
dc.description.sponsorship | Unión Europea. H2020 | |
dc.description.sponsorship | Ministerio de Ciencia e Innovación (MICINN) | |
dc.description.sponsorship | Ministerio de Economía y Competitividad (MINECO) | |
dc.description.sponsorship | Universidad San Pablo CEU (Emerging Group) | |
dc.description.status | pub | |
dc.eprint.id | https://eprints.ucm.es/id/eprint/44713 | |
dc.identifier.doi | 10.1002/jcp.25829 | |
dc.identifier.issn | 0021-9541, ESSN: 1097-4652 | |
dc.identifier.officialurl | http://onlinelibrary.wiley.com/doi/10.1002/jcp.25829/full | |
dc.identifier.uri | https://hdl.handle.net/20.500.14352/18086 | |
dc.issue.number | 12 | |
dc.journal.title | Journal of Cellular Physiology | |
dc.language.iso | eng | |
dc.page.final | 3621 | |
dc.page.initial | 3611 | |
dc.publisher | Wiley | |
dc.relation.projectID | VERDI (694160) | |
dc.relation.projectID | MAT2012-35556 | |
dc.relation.projectID | (MAT2013-43299-R, MAT2015-64831-R and MAT2016-75611-R ) | |
dc.relation.projectID | (FI12/00458) | |
dc.rights.accessRights | open access | |
dc.subject.cdu | 577.1 | |
dc.subject.cdu | 612.75 | |
dc.subject.keyword | Diabetes mellitus | |
dc.subject.keyword | Mechanotransduction | |
dc.subject.keyword | Osteoclast migration | |
dc.subject.keyword | Osteoclastogenesis | |
dc.subject.keyword | Osteocyte | |
dc.subject.ucm | Fisiología | |
dc.subject.ucm | Bioquímica (Biología) | |
dc.subject.unesco | 2411 Fisiología Humana | |
dc.subject.unesco | 2302 Bioquímica | |
dc.title | High glucose alters the secretome of mechanically stimulated osteocyte-like cells affecting osteoclast precursor recruitment and differentiation | |
dc.type | journal article | |
dc.volume.number | 232 | |
dspace.entity.type | Publication | |
relation.isAuthorOfPublication | 4b317058-0bd1-4fd8-afab-5fa79a4b7002 | |
relation.isAuthorOfPublication | 216318f7-e25a-4850-b122-856eb08b3e2f | |
relation.isAuthorOfPublication | d92c7075-3d31-45ec-a18d-35a5010ee8e1 | |
relation.isAuthorOfPublication | 791023b8-2531-44eb-ba01-56e3b7caa0cb | |
relation.isAuthorOfPublication.latestForDiscovery | 4b317058-0bd1-4fd8-afab-5fa79a4b7002 |
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