Stallion sperm selection prior to freezing using a modified colloid swim-up procedure without centrifugation

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dc.contributor.authorHidalgo Prieto, Manuel
dc.contributor.authorOrtiz Jaraba, Isabel
dc.contributor.authorDorado Martín, Jesús
dc.contributor.authorMorrel, Jane M
dc.contributor.authorGosálvez Berenguer, Jaime
dc.contributor.authorConsuegra González, Cesar
dc.contributor.authorDiaz Jimenez,María
dc.contributor.authorPereira Aguilar, Blasa
dc.contributor.authorCrespo Castejón, Francisco
dc.date.accessioned2024-01-31T15:38:00Z
dc.date.available2024-01-31T15:38:00Z
dc.date.issued2017-10
dc.description.abstractThe aims of this study were to: 1) develop a new method for stallion sperm selection using a modified swim-up procedure through a colloid and 2) evaluate its impact in good quality eja-culates from bad freezers in comparison to methods involving centrifugation such as single layer centrifugation and sperm washing. Ejaculates were processed before freezing using three dif-ferent procedures: sperm washing (SW), colloid single layer centrifugation (SLC) and a modified colloid swim-up (SU). After semen processing, sperm recovery rates were measured and sperm were frozen. Post-thaw sperm motility (assessed by computer-assisted sperm analysis), normal forms and plasma membrane integrity (evaluated under bright-field and fluorescence microscopy respectively), and DNA fragmentation (assessed by the Sperm-Halomax kit) were compared be-tween treatments. Sperm recovery rates were similar between SU and SLC but lower than SW. Sperm motility after thawing was lower in SU in comparison to SLC and SW, maybe due to the incomplete removal of seminal plasma before freezing. Sperm DNA fragmentation was lower in SU and SLC selection methods, particularly in SLC selected samples during the first 6 h of in-cubation. The remaining sperm parameters assessed were similar among treatments. In conclu-sion, SLC is more suitable than SW and SU to process stallion semen prior to freezing, in parti-cular when sperm DNA damage is suspected. Further studies are needed in order to determine the potential benefits of SU in samples where centrifugation is not necessary, such as epididymal sperm, ejaculate fractioning or post-thaw semen samples.
dc.description.departmentDepto. de Medicina y Cirugía Animal
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipDr JMM provided the Androcoll-E and Halotech DNA SL the Sperm-Halomax kits used in the experiments
dc.description.statuspub
dc.identifier.citation1) Hidalgo M, Ortiz I, Dorado J, Morrell JM, Gosálvez J, Consuegra C, Diaz-Jimenez M, Pereira B, Crespo F. Stallion sperm selection prior to freezing using a modified colloid swim-up procedure without centrifugation. Anim Reprod Sci. 2017 Oct;185:83-88
dc.identifier.doi10.1016/j.anireprosci.2017.08.005
dc.identifier.essn1873-2232
dc.identifier.issn0378-4320
dc.identifier.officialurlhttps://www.sciencedirect.com/science/article/pii/S037843201730057X?via%3Dihub
dc.identifier.pmid28827080
dc.identifier.relatedurlhttp://www.journals.elsevier.com/animal-reproduction-science/
dc.identifier.urihttps://hdl.handle.net/20.500.14352/97292
dc.journal.titleAnimal Rerpoduction Science
dc.language.isoeng
dc.page.final88
dc.page.initial83
dc.publisherElsevier
dc.relation.projectIDAGL-2013-42726-R (Secretaría de Estado de Investigación, Desarrollo e Innovación, Ministerio de Economía y Competitividad, Spain)
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsrestricted access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.keywordEquine
dc.subject.keywordSperm selection
dc.subject.keywordCryopreservation
dc.subject.ucmVeterinaria
dc.subject.unesco24 Ciencias de la Vida
dc.titleStallion sperm selection prior to freezing using a modified colloid swim-up procedure without centrifugation
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number185
dspace.entity.typePublication
relation.isAuthorOfPublicationd4b46bbe-e6cb-410e-8b74-b2361b41941d
relation.isAuthorOfPublication.latestForDiscoveryd4b46bbe-e6cb-410e-8b74-b2361b41941d
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