Acquisition of fertilization competence in guinea pig spermatozoa under different capacitation protocols

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dc.contributor.authorCañón Beltrán, Karina Esperanza
dc.contributor.authorCajas Suárez, Yulia Nathaly
dc.contributor.authorGonzález Martínez, María Encina
dc.contributor.authorFernández-González, Raul
dc.contributor.authorFierro, Natacha
dc.contributor.authorLorenzo González, Pedro Luis
dc.contributor.authorArias Álvarez, María
dc.contributor.authorGarcía García, Rosa María
dc.contributor.authorGutiérrez Adán, Alfonso
dc.contributor.authorRizos, Dimitri
dc.date.accessioned2024-07-04T11:35:49Z
dc.date.available2024-07-04T11:35:49Z
dc.date.issued2023-03-01
dc.description.abstractGuinea pig in vitro fertilization (IVF) are poorly developed due to the limited accessibility to oocytes and the lack of an efficient method of sperm capacitation. Thus, we aimed to evaluate different capacitation protocols that we validated through sperm analysis and using heterologous (He) IVF with zona-intact bovine oocytes. Spermatozoa of guinea pigs were collected and processed separately by 4 different protocols: A) Spermatozoa were obtained by flushing the lumen of one cauda epididymis and incubated in a minimal culture medium (MCM); B) One epididymis was placed in a prewarmed of M2 medium and gently minced with fine scissors. Spermatozoa were incubated in a modified human tubal fluid medium (HTF). In both protocols, the spermatozoa were capacitated at 37 °C under an atmosphere of 5% CO2 for 2 h. In the protocols C and D, the spermatozoa were collected by flushing the lumen of the cauda epididymis and selected by commercial density gradient Bovipure® (Nidacon Laboratories AB, Göthenborg, Sweden), according to the manufacturer's instructions. Then for Protocol C) spermatozoa were incubated in MCM medium supplemented with 10 mg/mL heparin (MCM-Hep); while for Protocol D) spermatozoa were incubated in FERT medium supplemented 10 mg/mL heparin (FERT-Hep). Incubation of C and D protocols were performed at 38.5 °C under an atmosphere of 5% CO2 for 2 h. Capacitation protocols C and D showed a higher percentage of viability, total and hyperactive-like motility, and acrosome reaction compared to protocols A and B. For this reason, protocols C and D were used for further He-IVF analysis. Guinea pig sperm and matured zona-intact bovine oocytes were co-incubated at 5% CO2 and 38.5 °C. Sperm-oocyte interaction was assessed at 2.5 h post-insemination (hpi) and pronuclear formation (PrF) were evaluated at 18, 20, 22, 24 and 26 hpi, while the cleavage rate was evaluated at 48 hpi. In protocol D, PrF was significantly higher than in protocol C (P ≤ 0.05) at every time point evaluated. Also, the cleavage rate at 48 hpi was higher (P ≤ 0.05) in He-IVF protocol D (69.8 ± 1.7%) compared to He-IVF protocol C (49.1 ± 1.1%). In conclusion, we determined the most adequate sperm capacitation conditions for guinea pig that allow zona-intact bovine oocyte penetration and lead to hybrid embryo formation, suggesting that these conditions could be optimal to develop IVF in guinea pigs.
dc.description.departmentSección Deptal. de Anatomía y Embriología (Veterinaria)
dc.description.departmentSección Deptal. de Fisiología (Veterinaria)
dc.description.departmentDepto. de Producción Animal
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia e Innovación (España)
dc.description.statuspub
dc.identifier.citationKarina Cañón-Beltrán, Yulia N. Cajas, Encina González, Raúl Fernández-González, Natacha Fierro, Pedro L. Lorenzo, María Arias-Álvarez, Rosa M. García-García, Alfonso Gutiérrez-Adán, Dimitrios Rizos, Acquisition of fertilization competence in guinea pig spermatozoa under different capacitation protocols, Theriogenology, Volume 198, 2023, Pp 231-240, https://doi.org/10.1016/j.theriogenology.2022.12.042.
dc.identifier.doi10.1016/j.theriogenology.2022.12.042
dc.identifier.essn1879-3231
dc.identifier.issn0093-691X
dc.identifier.officialurlhttps://doi.org/10.1016/j.theriogenology.2022.12.042
dc.identifier.urihttps://hdl.handle.net/20.500.14352/105623
dc.journal.titleTheriogenology
dc.language.isoeng
dc.page.final240
dc.page.initial231
dc.publisherElsevier
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-111641RB-I00/ES/VESICULAS EXTRACELULARES: MODULADORES DE LA INTERACCION MATERNO-EMBRIONARIA Y DE LA FERTILIDAD EN BOVINO/
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-093548-B-I00/ES/ANALISIS FUNCIONAL DE ISOFORMAS DE ARNM RELACIONADAS CON DESARROLLO EMBRIONARIO Y FERTILIDAD/
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.cdu636.09
dc.subject.keywordHeterologous IVF
dc.subject.keywordSperm capacitation
dc.subject.keywordZona pellucida
dc.subject.keywordFertilization
dc.subject.keywordGuinea pig
dc.subject.ucmVeterinaria
dc.subject.unesco3109 Ciencias Veterinarias
dc.titleAcquisition of fertilization competence in guinea pig spermatozoa under different capacitation protocols
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number198
dspace.entity.typePublication
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