Genomic and Functional Evaluation of Two Lacticaseibacillus paracasei and Two Lactiplantibacillus plantarum Strains, Isolated from a Rearing Tank of Rotifers (Brachionus plicatilis), as Probiotics for Aquaculture

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dc.contributor.authorContente, Diogo
dc.contributor.authorDíaz Formoso, Lara
dc.contributor.authorFeito Hermida, Javier
dc.contributor.authorHernández Cruza, Pablo Elpidio
dc.contributor.authorMuñoz Atienza, Estefanía
dc.contributor.authorBorrero Del Pino, Juan
dc.contributor.authorPoeta, Patrícia
dc.contributor.authorCintas Izarra, Luis Miguel
dc.contributor.editorFigueras, Antonio
dc.date.accessioned2024-05-08T18:39:37Z
dc.date.available2024-05-08T18:39:37Z
dc.date.issued2024-01-01
dc.descriptionAuthor Contributions: Conceptualization, L.M.C. and P.P.; methodology, D.C., L.D.-F., J.F. and E.M.-A.; software, D.C. and L.D.-F.; validation, J.F. and J.B.; formal analysis, D.C., J.F. and P.E.H.; investigation, D.C., L.D.-F., J.F. and E.M.-A.; resources, J.B., P.P. and L.M.C.; data curation, E.M.- A.; writing—original draft preparation, D.C.; writing—review and editing, L.M.C., P.P. and P.E.H.; visualization, D.C., J.F., P.E.H. and E.M.-A.; supervision, L.M.C. and P.P.; project administration, L.M.C. and J.B.; funding acquisition, L.M.C. and J.B. All authors have read and agreed to the published version of the manuscript.
dc.description.abstractAquaculture plays a crucial role in meeting the increasing global demand for food and protein sources. However, its expansion is followed by increasing challenges, such as infectious disease outbreaks and antibiotic misuse. The present study focuses on the genetic and functional analyses of two Lacticaseibacillus paracasei (BF3 and RT4) and two Lactiplantibacillus plantarum (BF12 and WT12) strains isolated from a rotifer cultivation tank used for turbot larviculture. Whole-genome sequencing (WGS) and bioinformatics analyses confirmed their probiotic potential, the absence of transferable antibiotic resistance genes, and the absence of virulence and pathogenicity factors. Bacteriocin mining identified a gene cluster encoding six plantaricins, suggesting their role in the antimicrobial activity exerted by these strains. In vitro cell-free protein synthesis (IV-CFPS) analyses was used to evaluate the expression of the plantaricin genes. The in vitro-synthesized class IIb (two-peptide bacteriocins) plantaricin E/F (PlnE/F) exerted antimicrobial activity against three indicator microorganisms, including the well-known ichthyopathogen Lactococcus garvieae. Furthermore, MALDI-TOF MS on colonies detected the presence of a major peptide that matches the dimeric form of plantaricins E (PlnE) and F (PlnF). This study emphasizes the importance of genome sequencing and bioinformatic analysis for evaluating aquaculture probiotic candidates. Moreover, it provides valuable insights into their genetic features and antimicrobial mechanisms, paving the way for their application as probiotics in larviculture, which is a major bottleneck in aquaculture.
dc.description.departmentSección Dptal. de Nutrición y Ciencia de los Alimentos (Veterinaria)
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia, Innovación y Universidades (España)
dc.description.statuspub
dc.identifier.citationContente, D.; Díaz-Formoso, L.; Feito, J.; Hernández, P.E.; Muñoz-Atienza, E.; Borrero, J.; Poeta, P.; Cintas, L.M. Genomic and Functional Evaluation of Two Lacticaseibacillus paracasei and Two Lactiplantibacillus plantarum Strains, Isolated from a Rearing Tank of Rotifers (Brachionus plicatilis), as Probiotics for Aquaculture. Genes 2024, 15, 64. https://doi.org/ 10.3390/genes15010064
dc.identifier.doi10.3390/genes15010064
dc.identifier.issn2073-4425
dc.identifier.officialurlhttps://doi.org/10.3390/genes15010064
dc.identifier.relatedurlhttps://www.mdpi.com/journal/genes
dc.identifier.urihttps://hdl.handle.net/20.500.14352/103836
dc.issue.number64
dc.journal.titleGenes
dc.language.isoeng
dc.publisherMDPI
dc.relation.projectIDinfo:eu-repo/grantAgreement/MCIU/RTI2018/094907-B-I00
dc.relation.projectIDinfo:eu-repo/grantAgreement/MCIU/PID2019/104808RA-I00
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu612.39
dc.subject.keywordAquaculture
dc.subject.keywordLarviculture
dc.subject.keywordRotifers (Brachionus plicatilis)
dc.subject.keywordProbiotics
dc.subject.keywordLactic acid bacteria
dc.subject.keywordLactobacilli
dc.subject.keywordWGS
dc.subject.keywordBioinformatics
dc.subject.keywordPlantaricins
dc.subject.ucmCiencias Biomédicas
dc.subject.unesco3104.06 Nutrición
dc.titleGenomic and Functional Evaluation of Two Lacticaseibacillus paracasei and Two Lactiplantibacillus plantarum Strains, Isolated from a Rearing Tank of Rotifers (Brachionus plicatilis), as Probiotics for Aquaculture
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number15
dspace.entity.typePublication
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relation.isAuthorOfPublication.latestForDiscovery48922a93-481a-488b-b69d-d70831dea16c
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Genomic and Functional Evaluation of Two Lacticaseibacillus paracasei and Two Lactiplantibacillus plantarum Strains, Isolated from a Rearing Tank of Rotifers (Brachionus plicatilis), as Probiotics for Aquaculture
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