Biocompatible Probes Based on Rare-Earth Doped Strontium Aluminates with Long-Lasting Phosphorescent Properties for In Vitro Optical IMAGING

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dc.contributor.authorCalatayud, David G.
dc.contributor.authorJardiel, Teresa
dc.contributor.authorCordero Oyonarte, Erica
dc.contributor.authorCaballero, Amador C.
dc.contributor.authorVillegas, Marina
dc.contributor.authorValle Noguera, Ana
dc.contributor.authorCruz Adalia, Aranzazu
dc.contributor.authorPeiteado, Marco
dc.date.accessioned2023-06-22T10:49:45Z
dc.date.available2023-06-22T10:49:45Z
dc.date.issued2022-03-21
dc.description.abstractIn recent decades, the demand for biomedical imaging tools has grown very rapidly as a key feature for biomedical research and diagnostic applications. Particularly, fluorescence imaging has gained increased attention as a non-invasive, inexpensive technique that allows real-time imaging. However, tissue auto-fluorescence under external illumination, together with a weak tissue penetration of low wavelength excitation light, largely restricts the application of the technique. Accordingly, new types of fluorescent labels are currently being investigated and, in this search, phosphorescent nanoparticles promise great potential, as they combine the interesting size-dependent properties of nanoscale materials with a long-lasting phosphorescence-type emission that allows optical imaging well after excitation (so avoiding autofluorescence). In this work, core-shell structures consisting of SrAlO:Eu,Dy luminescent cores encapsulated within a biocompatible silica shell were prepared, showing a green persistent phosphorescence with an afterglow time of more than 1000 s. A high-energy ball milling procedure was used to reduce the size of the starting phosphors to a size suitable for cellular uptake, while the silica coating was produced by a reverse micelle methodology that eventually allows the excitation and emission light to pass efficiently through the shell. Confocal fluorescence microscopy using HeLa cancer cells confirmed the potential of the all-ceramic composites produced as feasible labels for in vitro optical imaging.
dc.description.departmentDepto. de Inmunología, Oftalmología y ORL
dc.description.departmentDepto. de Medicina
dc.description.facultyFac. de Medicina
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia e Innovación (MICINN)
dc.description.sponsorshipMinisterio de Economía y Competitividad (MINECO)
dc.description.sponsorshipMinisterio de Ciencia e Innovación (MICINN)/FEDER
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/73369
dc.identifier.doi10.3390/ijms23063410
dc.identifier.issn1422-0067
dc.identifier.officialurlhttps://doi.org/10.3390/ijms23063410
dc.identifier.relatedurlhttps://www.mdpi.com/1422-0067/23/6/3410/htm
dc.identifier.urihttps://hdl.handle.net/20.500.14352/71741
dc.issue.number6
dc.journal.titleInternational Journal of Molecular Sciences
dc.language.isoeng
dc.page.initial3410
dc.publisherMPDI
dc.relation.projectIDPID2019-104118RB-C21
dc.relation.projectIDMAT2016-80182-R
dc.relation.projectIDRTI2018-093647-B-I00
dc.rightsAtribución 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/es/
dc.subject.keywordoptical bioimaging
dc.subject.keywordfluorescent labels
dc.subject.keywordceramic composites
dc.subject.keywordphosphorescent inorganics
dc.subject.keywordlong persistent luminescence
dc.subject.keywordcellular uptake
dc.subject.ucmInmunología
dc.subject.ucmÓptica y optometría
dc.subject.unesco2412 Inmunología
dc.subject.unesco2209 Óptica
dc.titleBiocompatible Probes Based on Rare-Earth Doped Strontium Aluminates with Long-Lasting Phosphorescent Properties for In Vitro Optical IMAGING
dc.typejournal article
dc.volume.number23
dspace.entity.typePublication
relation.isAuthorOfPublicationd7ccfedb-f3d6-43c6-b3c7-1196d375be31
relation.isAuthorOfPublicationae965912-b825-4a38-98db-737d69d3759a
relation.isAuthorOfPublication.latestForDiscoveryd7ccfedb-f3d6-43c6-b3c7-1196d375be31
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