Optimization of a Rabbit Dry Eye Model Induced by Topical Instillation of Benzalkonium Chloride

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dc.contributor.authorCarpena Torres, Carlos
dc.contributor.authorPintor Just, Jesús Jerónimo
dc.contributor.authorPérez de Lara, María Jesús
dc.contributor.authorHuete Toral, Fernando
dc.contributor.authorCrooke Álvarez, Almudena
dc.contributor.authorPastrana Robles, Cristina
dc.contributor.authorCarracedo Rodríguez, Juan Gonzalo
dc.date.accessioned2023-06-16T15:18:43Z
dc.date.available2023-06-16T15:18:43Z
dc.date.issued2020-05-30
dc.descriptionThis study was published in memory of Jesus Pintor, who passed away on 2 April 2019. This study has been self-funded.
dc.description.abstractPurpose. To optimize a rabbit dry eye model induced by topical instillation of benzalkonium chloride (BAC), reduce the days of instillation of the original model by increasing the concentration of BAC from 0.1% to 0.2%. Materials and Methods. An experimental, prospective, and randomized study was performed on 10 male New Zealand white rabbits, divided into two groups, considering both eyes: 5 rabbits as control (n = 10) and 5 rabbits with 0.2% BAC treatment (n = 10). Saline solution (control) and 0.2% BAC were instilled for 5 consecutive days, twice daily. Tear secretion with and without anesthesia, tear breakup time, tear osmolarity, corneal staining, conjunctival hyperemia, density of goblet cells, height of mucin cloud, and transcript levels of IL-6 were measured before and after the treatment. Results. After the instillation of 0.2% BAC for 5 consecutive days, there was a significant increase in tear secretion without anesthesia (P < 0.001), corneal staining (P < 0.001), conjunctival hyperemia (P < 0.001), and levels of IL-6 mRNA (P = 0.005) compared to the control group. Conversely, there was a decrease in tear secretion with anesthesia (P < 0.001), tear breakup time (P = 0.007), tear osmolarity (P < 0.001), density of goblet cells (P < 0.001), and height of mucin cloud (P < 0.001). Conclusions. )e topical instillation of 0.2% BAC for 5 consecutive days, twice daily, was a proper procedure to induce a rabbit dry eye model, reducing the number of days of instillation compared to the original model (14 days).en
dc.description.departmentDepto. de Optometría y Visión
dc.description.departmentUnidad Docente de Bioquímica y Biología Molecular
dc.description.facultyFac. de Óptica y Optometría
dc.description.refereedTRUE
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/60780
dc.identifier.citationCarpena-Torres, C., Pintor Just, J. J., Pérez de Lara, M. J. et al. «Optimization of a Rabbit Dry Eye Model Induced by Topical Instillation of Benzalkonium Chloride». Journal of Ophthalmology, vol. 2020, mayo de 2020, pp. 1-10. DOI.org (Crossref), https://doi.org/10.1155/2020/7204951.
dc.identifier.doi10.1155/2020/7204951
dc.identifier.issn2090-004X
dc.identifier.officialurlhttps://doi.org/10.1155/2020/7204951
dc.identifier.relatedurlhttps://www.hindawi.com/journals/joph/2020/7204951/#copyright
dc.identifier.urihttps://hdl.handle.net/20.500.14352/6300
dc.journal.titleJournal of Ophthalmology
dc.language.isoeng
dc.page.initial10 p.
dc.publisherHindawi Publishing Corporation
dc.rightsAtribución 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/es/
dc.subject.cdu617.764.1
dc.subject.keywordDry Eye
dc.subject.keywordRabbit
dc.subject.keywordTopical instillation
dc.subject.keywordBenzalkonium Chloride
dc.subject.ucmOftalmología
dc.subject.ucmAnatomía ocular
dc.subject.ucmÓptica fisiológica
dc.subject.unesco3201.09 Oftalmología
dc.titleOptimization of a Rabbit Dry Eye Model Induced by Topical Instillation of Benzalkonium Chlorideen
dc.typejournal article
dc.volume.number2020
dspace.entity.typePublication
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