Electrochemical immunosensor for simultaneous determination of interleukin-1 beta and tumor necrosis factor alpha in serum and saliva using dual screen printed electrodes modified with functionalized double–walled carbon nanotubes

Simple item page
dc.contributor.authorSánchez Tirado, Esther
dc.contributor.authorSalvo, Coral
dc.contributor.authorGonzález Cortés, Araceli
dc.contributor.authorYáñez Sedeño, Paloma
dc.contributor.authorLanga, Fernando
dc.contributor.authorPingarrón Carrazón, José Manuel
dc.date.accessioned2023-06-17T22:05:25Z
dc.date.available2023-06-17T22:05:25Z
dc.date.issued2016-12-23
dc.description.abstractDual screen-printed carbon electrodes modified with 4-carboxyphenyl-functionalized double-walled carbon nanotubes (HOOC-Phe-DWCNTs/SPCEs) have been used as scaffolds for the preparation of electrochemical immunosensors for the simultaneous determination of the cytokines Interleukin-1b (IL-1b) and factor necrosis tumor a (TNF-a). IL-1b. Capture antibodies were immobilized onto HOOC-Phe DWCNTs/SPCEs in an oriented form making using the commercial polymeric coating Mix&Go™. Sandwich type immunoassays with amperometric signal amplification through the use of poly-HRPstreptavidin conjugates and H2O2 as HRP substrate and hydroquinone as redox mediator were implemented. Upon optimization of the experimental variables affecting the immunosensor performance, the dual immunosensor allows ranges of linearity extending between 0.5 and 100 pg/mL and from 1 to 200 pg/mL for IL-1b and TNF-a, respectively, these ranges being adequate for the determination of the cytokines in clinical samples. The achieved limits of detection were 0.38 pg/mL (IL-1b) and 0.85 pg/mL (TNF-a). In addition, the dual immunosensor exhibits excellent reproducibility of the measurements, storage stability of the anti-IL-Phe-DWCNTs/SPCE and anti-TNF-Phe-DWCNTs/SPCE conjugates, and selectivity as well as negligible cross-talking. The dual immunosensor was applied to the simultaneous determination of IL-1b and TNF-a in human serum spiked at clinically relevant concentration levels and in real saliva samples.
dc.description.departmentDepto. de Química Analítica
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía y Competitividad (MINECO)
dc.description.sponsorshipComunidad de Madrid
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/44483
dc.identifier.doi10.1016/j.aca.2016.12.034
dc.identifier.issn0003-2670
dc.identifier.officialurlhttps://www.sciencedirect.com/science/article/pii/S0003267016315094
dc.identifier.urihttps://hdl.handle.net/20.500.14352/18045
dc.journal.titleAnalytica Chimica Acta
dc.language.isoeng
dc.page.final73
dc.page.initial66
dc.publisherElsevier
dc.relation.projectIDCTQ2015-70023-R , CTQ2015- 71955-REDT
dc.relation.projectIDNANOAVANSENS (S2013/MT-3029)
dc.rights.accessRightsopen access
dc.subject.cdu543
dc.subject.keywordinterleukin-1 beta
dc.subject.keywordtumor necrosis factor-alpha
dc.subject.keywordelectrochemical immunosensor
dc.subject.keywordmultiplex
dc.subject.keyworddouble-walled carbon nanotubes
dc.subject.keywordsaliva.
dc.subject.ucmQuímica analítica (Química)
dc.subject.unesco2301 Química Analítica
dc.titleElectrochemical immunosensor for simultaneous determination of interleukin-1 beta and tumor necrosis factor alpha in serum and saliva using dual screen printed electrodes modified with functionalized double–walled carbon nanotubes
dc.typejournal article
dc.volume.number959
dspace.entity.typePublication
relation.isAuthorOfPublication8ce0e95b-049c-4147-be76-110e258443a1
relation.isAuthorOfPublication8a730734-cdf5-4b59-b017-12e2320c872e
relation.isAuthorOfPublication8808f99c-5f56-4562-839a-517626c76dad
relation.isAuthorOfPublication.latestForDiscovery8ce0e95b-049c-4147-be76-110e258443a1
Download
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Paper dual saliva revised (clean) (1)Paloma.pdf
Size:
748 KB
Format:
Adobe Portable Document Format
downoload Download
Collections
Artículos