Synergistic effect of Si-hydroxyapatite coating and VEGF adsorption on Ti6Al4V-ELI scaffolds for bone regeneration in an osteoporotic bone environment.

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dc.contributor.authorIzquierdo Barba, Isabel
dc.contributor.authorSantos-Ruiz, L
dc.contributor.authorBecerra, J
dc.contributor.authorFeito Castellano, María José
dc.contributor.authorFernandez-Villa, D
dc.contributor.authorSerrano, M.C
dc.contributor.authorDiaz-Gúemes, I
dc.contributor.authorFernandez-Tome, B
dc.contributor.authorEnciso, S
dc.contributor.authorSanchez-Margallo, F.M
dc.contributor.authorMonopoli, D
dc.contributor.authorAlfonso, H
dc.contributor.authorPortolés Pérez, María Teresa
dc.contributor.authorArcos Navarrete, Daniel
dc.contributor.authorVallet Regí, María Dulce Nombre
dc.date.accessioned2023-06-17T13:18:16Z
dc.date.available2023-06-17T13:18:16Z
dc.date.issued2018-11-13
dc.descriptionRESEARCHER ID M-9921-2014 (Isabel Izquierdo Barba) ORCID 0000-0002-4139-4646 (Isabel Izquierdo Barba) RESEARCHER ID U-1678-2017 (María Teresa Portolés Pérez) ORCID 0000-0002-9681-0184 (María Teresa Portolés Pérez) RESEARCHER ID L-6167-2014 (Daniel Arcos Navarrete) ORCID 0000-0002-5367-7272 (Daniel Arcos Navarrete) RESEARCHER ID M-3378-2014 (María Vallet Regí) ORCID 0000-0002-6104-4889 (María Vallet Regí)
dc.description.abstractThe osteogenic and angiogenic responses to metal macroporous scaffolds coated with silicon substituted hydroxyapatite (SiHA) and decorated with vascular endothelial growth factor (VEGF) have been evaluated in vitro and in vivo. Ti6Al4V-ELI scaffolds were prepared by electron beam melting and subsequently coated with Ca10(PO4)5.6(SiO4)0.4(OH)1.6 following a dip coating method. In vitro studies demonstrated that SiHA stimulates the proliferation of MC3T3-E1 pre-osteoblastic cells, whereas the adsorption of VEGF stimulates the proliferation of EC2 mature endothelial cells. In vivo studies were carried out in an osteoporotic sheep model, evidencing that only the simultaneous presence of both components led to a significant increase of new tissue formation in osteoporotic bone. STATEMENT OF SIGNIFICANCE Reconstruction of bones after severe trauma or tumors extirpation is one of the most challenging tasks in the field of orthopedic surgery. This scenario is even more complicated in the case of osteoporotic patients, since their bone regeneration capability is decreased. In this work we present a porous implant that promotes bone regeneration even in osteoporotic bone. By coating the implant with an osteogenic bioceramics such as silicon substituted hydroxyapatite and subsequent adsorption of vascular endothelial growth factor, these implants stimulate the bone ingrowth when they are implanted in osteoporotic sheep.
dc.description.departmentDepto. de Química en Ciencias Farmacéuticas
dc.description.facultyFac. de Farmacia
dc.description.refereedTRUE
dc.description.sponsorshipUnión Europea. H2020
dc.description.sponsorshipMinisterio de Economía y Competitividad (MINECO)
dc.description.sponsorshipInstituto de Salud Carlos III
dc.description.statusinpress
dc.eprint.idhttps://eprints.ucm.es/id/eprint/50139
dc.identifier.doi10.1016/j.actbio.2018.11.017
dc.identifier.issn1742-7061
dc.identifier.officialurlhttps://www.elsevier.com/es-es
dc.identifier.relatedurlhttp://www.ucm.es/valletregigroup
dc.identifier.urihttps://hdl.handle.net/20.500.14352/12928
dc.journal.titleActa Biomaterialia
dc.language.isoeng
dc.publisherELSEVIER SCIENCE BV
dc.relation.projectIDVERDI (694160)
dc.relation.projectIDMAT2015-64831-R; MAT2016-75611-R
dc.relation.projectIDR-RD12-0019-0032
dc.rights.accessRightsopen access
dc.subject.cdu615.46
dc.subject.cdu546
dc.subject.keywordBone regeneration
dc.subject.keywordSilicon hydroxyapatite
dc.subject.keywordVEGF
dc.subject.keywordOsteoporosis
dc.subject.keywordScaffold
dc.subject.ucmMateriales
dc.subject.ucmQuímica inorgánica (Farmacia)
dc.subject.unesco3312 Tecnología de Materiales
dc.titleSynergistic effect of Si-hydroxyapatite coating and VEGF adsorption on Ti6Al4V-ELI scaffolds for bone regeneration in an osteoporotic bone environment.
dc.typejournal article
dspace.entity.typePublication
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relation.isAuthorOfPublication216318f7-e25a-4850-b122-856eb08b3e2f
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relation.isAuthorOfPublication.latestForDiscovery216318f7-e25a-4850-b122-856eb08b3e2f
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