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Preparation and characterization of PBS (Polybutylene Succinate) nanoparticles containing cannabidiol (CBD) for anticancer application

dc.contributor.authorFreitas Freire, Natália
dc.contributor.authorCordani, Marco
dc.contributor.authorAparicio Blanco, Juan
dc.contributor.authorFraguas Sánchez, Ana Isabel
dc.contributor.authorDutra, Luciana
dc.contributor.authorPinto, Martina Costa Cerqueira
dc.contributor.authorZarrabi, Ali
dc.contributor.authorPinto, José Carlos
dc.contributor.authorVelasco Díez, Guillermo
dc.contributor.authorFialho, Rosana
dc.date.accessioned2025-02-03T14:07:39Z
dc.date.available2025-02-03T14:07:39Z
dc.date.issued2024
dc.descriptionThis study was financed by Faperj and the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001, CAPES-PRINT 88887.569110/2020-00 and 88887.568544/2020-00. Marco Cordani is supported with a Ramon y Cajal grant (RYC2021-031003-I) from the Spanish Ministry of Science and Innovation, Agencia Estatal de Investigación (MCIN/AEI/10.13039/501100011033), and European Union Next Generation (EU/PRTR). Guillermo Velasco is supported by the Instituto de Salud Carlos III (ISCIII) and confounded by the European Regional Development Fund (ERDF), “A way to make Europe”, grant number PI18/00442 integrated into the State Plan for R & D + I 2017-2020 and grant number PI21/00343 integrated into the State Plan for R & D + I 2021-2023, by the European Commission through the Horizon 2020 European Training Networks program, grant number H2020-MSCA-ITN-308 2016 721532 and by the Madrid Region Government Network Program in Biosciences, grant number S2022/BMD-7434 (ASAP-CM).
dc.description.abstractCannabidiol (CBD), a major constituent of Cannabis sativa, has demonstrated a broad range of therapeutic properties in human studies. Notably, CBD has shown anticancer activity in preclinical cancer models. However, its low water solubility poses challenges for bioavailability, necessitating the development of drug delivery systems to enhance its efficacy. This study aimed to create CBD-loaded Poly (butylene succinate) (PBS) nanoparticles and evaluate their effectiveness in in vitro cancer models. The nanoparticles, with an average size of 175 nm, were produced using a modified double emulsion/solvent evaporation technique. The release profile of CBD from the nanoparticles exhibited an initial rapid release followed by a slower sustained release. Cytotoxicity assays demonstrated that the CBD-PBS nanoparticles retained the anticancer effects of free CBD, selectively reducing the viability of cancer cell lines without affecting non-transformed fibroblasts. Additionally, the nanoformulation modulated key cellular pathways, as indicated by decreased AKT phosphorylation and increased LC3-II levels, suggesting that the encapsulated CBD preserved its ability to induce autophagy-mediated cell death in cancer cells. The nanoformulation also effectively inhibited cell migration in highly invasive prostate cancer cells, mirroring the effects of free CBD, while not impacting the migration of non-tumoral fibroblasts. These results underscore the therapeutic potential of this CBD nanoformulation, setting the stage for further in vivo investigations.
dc.description.departmentDepto. de Bioquímica y Biología Molecular
dc.description.departmentDepto. de Farmacia Galénica y Tecnología Alimentaria
dc.description.facultyFac. de Farmacia
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipFundação Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil)
dc.description.sponsorshipMinisterio de Ciencia e Innovación (España)
dc.description.sponsorshipEuropean Commission
dc.description.sponsorshipInstituto de Salud Carlos III
dc.description.sponsorshipComunidad de Madrid
dc.description.statuspub
dc.identifier.citationFreire, N. F., Cordani, M., Aparicio-Blanco, J., Fraguas Sanchez, A. I., Dutra, L., Pinto, M. C. C., Zarrabi, A., Pinto, J. C., Velasco, G., & Fialho, R. (2024). Preparation and characterization of PBS (Polybutylene Succinate) nanoparticles containing cannabidiol (CBD) for anticancer application. Journal of Drug Delivery Science and Technology, 97, 105833. https://doi.org/10.1016/j.jddst.2024.105833
dc.identifier.doi10.1016/j.jddst.2024.105833
dc.identifier.essn2588-8943
dc.identifier.issn1773-2247
dc.identifier.officialurlhttps://doi.org/10.1016/j.jddst.2024.105833
dc.identifier.relatedurlhttps://www.sciencedirect.com/science/article/pii/S1773224724005021?via%3Dihub
dc.identifier.urihttps://hdl.handle.net/20.500.14352/117699
dc.journal.titleJournal of Drug Delivery Science and Technology
dc.language.isoeng
dc.publisherElsevier
dc.relation.projectIDinfo:eu-repo/grantAgreement/CAPES//88887.569110%2F2020-00/
dc.relation.projectIDinfo:eu-repo/grantAgreement/CAPES//88887.568544/2020-00./
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.cdu582.630.1
dc.subject.cdu633.888
dc.subject.cdu615.011
dc.subject.keywordPoly-butylene succinate
dc.subject.keywordEnvironmentally friendly polymers
dc.subject.keywordNanoparticles
dc.subject.keywordDrug delivery
dc.subject.keywordCannabidiol
dc.subject.keywordCancer cells
dc.subject.ucmBioquímica (Biología)
dc.subject.ucmFarmacología (Farmacia)
dc.subject.ucmFisiología animal (Farmacia)
dc.subject.unesco2403 Bioquímica
dc.subject.unesco3302 Tecnología Bioquímica
dc.subject.unesco2302.22 Farmacología Molecular
dc.subject.unesco3207.13 Oncología
dc.titlePreparation and characterization of PBS (Polybutylene Succinate) nanoparticles containing cannabidiol (CBD) for anticancer application
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number97
dspace.entity.typePublication
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relation.isAuthorOfPublication4a33b5e2-6540-4927-ab0d-bc37f5cd8b5b
relation.isAuthorOfPublication.latestForDiscoveryf61da389-972a-4336-8e1f-f3fe854c9c9f

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