Activation of Th lymphocytes alters pattern expression and cellular location of VIP receptors in healthy donors and early arthritis patients

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dc.contributor.authorVillanueva Romero, Raúl
dc.contributor.authorGutiérrez Cañas, Irene
dc.contributor.authorCarrión Caballo, Mar
dc.contributor.authorGonzález Álvaro, Isidoro
dc.contributor.authorRodríguez Frade, José Miguel
dc.contributor.authorMellado, Mario
dc.contributor.authorMartínez Mora, María Del Carmen
dc.contributor.authorGomáriz, Rosa P.
dc.contributor.authorJuarranz Moratilla, Yasmina
dc.date.accessioned2023-06-17T13:26:25Z
dc.date.available2023-06-17T13:26:25Z
dc.date.issued2019-05-14
dc.description.abstractVasoactive Intestinal Peptide (VIP) is an important immunomodulator of CD4+ cells in normal and pathological conditions, which exerts its anti-infammatory and immunomodulatory actions through VPAC receptors, VPAC1 and VPAC2. Only a decrease in the expression of VPAC1 mRNA on Th cells upon activation has been reported. Thus, the deepening in the knowledge of the behavior of these receptors may contribute to the design of new therapies based on their activation and/or blockade. In this study, we describe the expression pattern, cellular location and functional role of VIP receptors during the activation of human Th cells in healthy conditions and in early arthritis (EA). The protein expression pattern of VPAC1 did not change with the activation of Th lymphocytes, whereas VPAC2 was up-regulated. In resting cells, VPAC1 was located on the plasma membrane and nucleus, whereas it only appeared in the nucleus in activated cells. VPAC2 was always found in plasma membrane location. VIP receptors signaled through a PKA-dependent pathway in both conditions, and also by a PKAindependent pathway in activated cells. Both receptors exhibit a potent immunomodulatory capacity by controlling the pathogenic profle and the activation markers of Th cells. These results highlight a novel translational view in infammatory/autoimmune diseases.en
dc.description.departmentDepto. de Biología Celular
dc.description.facultyFac. de Ciencias Biológicas
dc.description.refereedTRUE
dc.description.sponsorshipInstituto de Salud Carlos III/Fondo Europeo de Desarrollo Regional
dc.description.sponsorshipRedes Temáticas de Investigación Cooperativa
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/56620
dc.identifier.citationVillanueva Romero, R., Gutiérrez Cañas, I., Carrión Caballo, M. et al. «Activation of Th Lymphocytes Alters Pattern Expression and Cellular Location of VIP Receptors in Healthy Donors and Early Arthritis Patients». Scientific Reports, vol. 9, n.o 1, mayo de 2019. Crossref, https://doi.org/10.1038/s41598-019-43717-2.
dc.identifier.doi10.1038/s41598-019-43717-2
dc.identifier.essn2045-2322
dc.identifier.officialurlhttps//doi.org/10.1038/s41598-019-43717-2
dc.identifier.relatedurlhttps://www.nature.com/articles/s41598-019-43717-2
dc.identifier.urihttps://hdl.handle.net/20.500.14352/13486
dc.issue.number7383
dc.journal.titleScientific Reports
dc.language.isoeng
dc.page.final14
dc.page.initial1
dc.publisherNature Research
dc.relation.projectID(RD16/0012/0008; RD16/0012/0006; RD16/0012/0011)
dc.relation.projectID(PI12/00758, PI14/00477 and PI17/0027)
dc.rightsAtribución 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/es/
dc.subject.cdu577.112.6
dc.subject.cdu616.72-002
dc.subject.keywordTh lymphocytes
dc.subject.keywordVasoactive Intestinal Peptide
dc.subject.keywordArthritis
dc.subject.ucmInmunología
dc.subject.ucmReumatología
dc.subject.ucmBioquímica (Biología)
dc.subject.unesco2412 Inmunología
dc.subject.unesco3205.09 Reumatología
dc.subject.unesco2302 Bioquímica
dc.titleActivation of Th lymphocytes alters pattern expression and cellular location of VIP receptors in healthy donors and early arthritis patientsen
dc.typejournal article
dc.volume.number9
dspace.entity.typePublication
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