Rapid micromotor-based naked-eye immunoassay
dc.contributor.author | Esteban Fernandez de Avila, Berta | |
dc.contributor.author | Zhao, Mingjiao | |
dc.contributor.author | Campuzano, Susana | |
dc.contributor.author | Ricci, Francesco | |
dc.contributor.author | Pingarrón Carrazón, José Manuel | |
dc.contributor.author | Mascini, Marcelo | |
dc.contributor.author | Wang, Joseph | |
dc.date.accessioned | 2023-06-17T22:44:31Z | |
dc.date.available | 2023-06-17T22:44:31Z | |
dc.date.issued | 2017-05-15 | |
dc.description.abstract | A dynamic micromotor-based immunoassay, exemplified by cortisol detection, based on the use of tubular micromotors functionalized with a specific antibody is described. The use of antibody-functionalized micromotors offers huge acceleration of both direct and competitive cortisol immunoassays, along with greatly enhanced sensitivity of direct and competitive immunoassays. The dramatically improved speed and sensitivity reflect the greatly increased likelihood of antibody-cortisol contacts and fluid mixing associated with the dynamic movement of these microtube motors and corresponding bubble generation that lead to a highly efficient and rapid recognition process. Rapid naked-eye detection of cortisol in the sample is achieved in connection to use of horseradish peroxidase (HRP) tag and TMB/H2O2 system. Key parameters of the competitive immunoassay (e.g., incubation time and reaction volume) were optimized. This fast visual micromotor-based sensing approach enables “on the move” specific detection of the target cortisol down to 0.1 μg mL−1 in just 2 min, using ultrasmall (50 µL) sample volumes. | |
dc.description.department | Depto. de Química Analítica | |
dc.description.faculty | Fac. de Ciencias Químicas | |
dc.description.refereed | TRUE | |
dc.description.sponsorship | Unión Europea. H2020 | |
dc.description.sponsorship | Defense Threat Reduction Agency Joint Science and Technology Office for Chemical and Biological Defense | |
dc.description.status | pub | |
dc.eprint.id | https://eprints.ucm.es/id/eprint/56685 | |
dc.identifier.doi | 10.1016/j.talanta.2017.02.068 | |
dc.identifier.issn | 0039-9140 | |
dc.identifier.officialurl | http://www.sciencedirect.com/science/article/pii/S0039914017302825 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14352/18814 | |
dc.journal.title | Talanta | |
dc.language.iso | eng | |
dc.page.final | 657 | |
dc.page.initial | 651 | |
dc.publisher | Elsevier | |
dc.relation.projectID | APTASENS (656687) | |
dc.relation.projectID | (Grants nos. HDTRA1-13-1–0002 and HDTRA1-14-1–0064) | |
dc.rights.accessRights | open access | |
dc.subject.cdu | 543 | |
dc.subject.keyword | Cortisol | |
dc.subject.keyword | Real-time sensing | |
dc.subject.keyword | Micromotor | |
dc.subject.keyword | Antibody | |
dc.subject.keyword | Naked-eye detection | |
dc.subject.ucm | Química analítica (Química) | |
dc.subject.unesco | 2301 Química Analítica | |
dc.title | Rapid micromotor-based naked-eye immunoassay | |
dc.type | journal article | |
dc.volume.number | 167 | |
dspace.entity.type | Publication | |
relation.isAuthorOfPublication | 8808f99c-5f56-4562-839a-517626c76dad | |
relation.isAuthorOfPublication.latestForDiscovery | 8808f99c-5f56-4562-839a-517626c76dad |