Rapid micromotor-based naked-eye immunoassay

Simple item page
dc.contributor.authorEsteban Fernandez de Avila, Berta
dc.contributor.authorZhao, Mingjiao
dc.contributor.authorCampuzano, Susana
dc.contributor.authorRicci, Francesco
dc.contributor.authorPingarrón Carrazón, José Manuel
dc.contributor.authorMascini, Marcelo
dc.contributor.authorWang, Joseph
dc.date.accessioned2023-06-17T22:44:31Z
dc.date.available2023-06-17T22:44:31Z
dc.date.issued2017-05-15
dc.description.abstractA dynamic micromotor-based immunoassay, exemplified by cortisol detection, based on the use of tubular micromotors functionalized with a specific antibody is described. The use of antibody-functionalized micromotors offers huge acceleration of both direct and competitive cortisol immunoassays, along with greatly enhanced sensitivity of direct and competitive immunoassays. The dramatically improved speed and sensitivity reflect the greatly increased likelihood of antibody-cortisol contacts and fluid mixing associated with the dynamic movement of these microtube motors and corresponding bubble generation that lead to a highly efficient and rapid recognition process. Rapid naked-eye detection of cortisol in the sample is achieved in connection to use of horseradish peroxidase (HRP) tag and TMB/H2O2 system. Key parameters of the competitive immunoassay (e.g., incubation time and reaction volume) were optimized. This fast visual micromotor-based sensing approach enables “on the move” specific detection of the target cortisol down to 0.1 μg mL−1 in just 2 min, using ultrasmall (50 µL) sample volumes.
dc.description.departmentDepto. de Química Analítica
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipUnión Europea. H2020
dc.description.sponsorshipDefense Threat Reduction Agency Joint Science and Technology Office for Chemical and Biological Defense
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/56685
dc.identifier.doi10.1016/j.talanta.2017.02.068
dc.identifier.issn0039-9140
dc.identifier.officialurlhttp://www.sciencedirect.com/science/article/pii/S0039914017302825
dc.identifier.urihttps://hdl.handle.net/20.500.14352/18814
dc.journal.titleTalanta
dc.language.isoeng
dc.page.final657
dc.page.initial651
dc.publisherElsevier
dc.relation.projectIDAPTASENS (656687)
dc.relation.projectID(Grants nos. HDTRA1-13-1–0002 and HDTRA1-14-1–0064)
dc.rights.accessRightsopen access
dc.subject.cdu543
dc.subject.keywordCortisol
dc.subject.keywordReal-time sensing
dc.subject.keywordMicromotor
dc.subject.keywordAntibody
dc.subject.keywordNaked-eye detection
dc.subject.ucmQuímica analítica (Química)
dc.subject.unesco2301 Química Analítica
dc.titleRapid micromotor-based naked-eye immunoassay
dc.typejournal article
dc.volume.number167
dspace.entity.typePublication
relation.isAuthorOfPublication8808f99c-5f56-4562-839a-517626c76dad
relation.isAuthorOfPublication.latestForDiscovery8808f99c-5f56-4562-839a-517626c76dad
Download
Collections
Artículos