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A useful tool for the safe diagnosis and control of the two main pandemics of the XXI century: COVID-19 and African Swine Fever disease

dc.contributor.authorBarroso Arévalo, Sandra
dc.contributor.authorDíaz Frutos, Marta
dc.contributor.authorKosowska, Aleksandra
dc.contributor.authorPérez Sancho, Marta
dc.contributor.authorDomínguez Rodríguez, Lucas José
dc.contributor.authorSánchez-Vizcaíno Rodríguez, José Manuel
dc.contributor.editorDouglas Gladue
dc.date.accessioned2024-08-21T08:54:51Z
dc.date.available2024-08-21T08:54:51Z
dc.date.issued2023-03-06
dc.descriptionAbout the Authors Sandra Barroso-Arévalo. ROLES Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Writing – original draft, Writing – review & editing Marta Díaz-Frutos. ROLES Conceptualization, Investigation, Methodology, Writing – review & editing Aleksandra Kosowska. ROLES Conceptualization, Investigation, Methodology, Writing – review & editing Marta Pérez-Sancho. ROLES Conceptualization, Methodology Lucas Domínguez. ROLES Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Validation, Writing – review & editing José Manuel Sánchez-Vizcaíno. ROLES Conceptualization, Funding acquisition, Investigation, Writing – review & editing
dc.description.abstractThe COVID-19 pandemic and the disease triggered by the African Swine Fever virus are currently two of the main problems regarding public and animal health, respectively. Although vaccination seems to be the ideal tool for controlling these diseases, it has several limitations. Therefore, early detection of the pathogen is critical in order to apply preventive and control measures. Real-time PCR is the main technique used for the detection of both viruses, which requires previous processing of the infectious material. If the potentially infected sample is inactivated at the time of sampling, the diagnosis will be accelerated, impacting positively on the diagnosis and control of the disease. Here, we evaluated the inactivation and preservation properties of a new surfactant liquid for non-invasive and environmental sampling of both viruses. Our results demonstrated that the surfactant liquid effectively inactivates SARS-CoV-2 and African Swine Fever virus in only five minutes, and allows for the preservation of the genetic material for long periods even at high temperatures such as 37°C. Hence, this methodology is a safe and useful tool for recovering SARS-CoV-2 and African Swine Fever virus RNA/DNA from different surfaces and skins, which has significant applied relevance in the surveillance of both diseases.
dc.description.departmentDepto. de Sanidad Animal
dc.description.facultyCentro de Vigilancia Sanitaria Veterinaria (VISAVET)
dc.description.refereedTRUE
dc.description.sponsorshipEuropean Union
dc.description.sponsorshipComunidad de Madrid
dc.description.statuspub
dc.identifier.citationBarroso-Arévalo S, Díaz-Frutos M, Kosowska A, Pérez-Sancho M, Domínguez L, Sánchez-Vizcaíno JM (2023) A useful tool for the safe diagnosis and control of the two main pandemics of the XXI century: COVID-19 and African Swine Fever disease. PLoS ONE 18(3): e0282632. https://doi.org/10.1371/journal.pone.0282632
dc.identifier.doi10.1371/journal.pone.0282632
dc.identifier.issn1932-6203
dc.identifier.officialurlhttps://doi.org/10.1371/journal.pone.0282632
dc.identifier.pmid36877705
dc.identifier.relatedurlhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0282632
dc.identifier.urihttps://hdl.handle.net/20.500.14352/107552
dc.issue.number3
dc.journal.titlePLoS ONE
dc.language.isoeng
dc.relation.projectIDH2020 VACDIVA 862874
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu636.09
dc.subject.ucmVeterinaria
dc.subject.unesco3109 Ciencias Veterinarias
dc.titleA useful tool for the safe diagnosis and control of the two main pandemics of the XXI century: COVID-19 and African Swine Fever disease
dc.typejournal article
dc.type.hasVersionAM
dc.volume.number18
dspace.entity.typePublication
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relation.isAuthorOfPublication94b7d8ef-e913-46ee-beeb-2d37b3caeb7f
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relation.isAuthorOfPublication.latestForDiscovery7416bf9e-a5a1-4ef0-8554-e45d89b1c44c

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A useful tool for the safe diagnosis and control of the two main pandemics of the XXI century: COVID-19 and African Swine Fever disease

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