Brain Mapping of Ghrelin O-Acyltransferase in Goldfish (Carassius Auratus): Novel Roles for the Ghrelinergic System in Fish?

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dc.contributor.authorBlanco Imperiali, Ayelén M.
dc.contributor.authorSánchez Bretano, Aída
dc.contributor.authorDelgado Saavedra, María Jesús
dc.contributor.authorValenciano González, Ana Isabel
dc.date.accessioned2023-06-18T05:46:48Z
dc.date.available2023-06-18T05:46:48Z
dc.date.issued2016
dc.description.abstractGhrelin O-acyltransferase (GOAT) is the enzyme responsible for acylation of ghrelin, a gut-brain hormone with important roles in many physiological functions in vertebrates. Many aspects of GOAT remain to be elucidated, especially in fish, and particularly its anatomical distribution within the different brain areas has never been reported to date. The present study aimed to characterize the brain mapping of GOAT using RT-qPCR and immunohistochemistry in a teleost, the goldfish (Carassius auratus). Results show that goat transcripts are expressed in different brain areas of the goldfish, with the highest levels in the vagal lobe. Using immunohistochemistry, we also report the presence of GOAT immunoreactive cells in different encephalic areas, including the telencephalon, some hypothalamic nuclei, pineal gland, optic tectum and cerebellum, although they are especially abundant in the hindbrain. Particularly, an important signal is observed in the vagal lobe and some fiber tracts of the brainstem, such as the medial longitudinal fasciculus, Mauthneri fasciculus, secondary gustatory tract and spinothalamic tract. Most of the forebrain areas where GOAT is detected, particularly the hypothalamic nuclei, also express the ghs-r1a ghrelin receptor and other appetite-regulating hormones (e.g., orexin and NPY), supporting the role of ghrelin as a modulator of food intake and energy balance in fish. Present results are the first report on the presence of GOAT in the brain using imaging techniques. The high presence of GOAT in the hindbrain is a novelty, and point to possible new functions for the ghrelinergic system in fish.
dc.description.departmentDepto. de Genética, Fisiología y Microbiología
dc.description.facultyFac. de Ciencias Biológicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía y Competitividad (MINECO)
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/42535
dc.identifier.doi10.1002/ar.23346
dc.identifier.issn0003-276X
dc.identifier.officialurlhttp://onlinelibrary.wiley.com/
dc.identifier.urihttps://hdl.handle.net/20.500.14352/23317
dc.journal.titleAnatomical Record
dc.language.isoeng
dc.page.final758
dc.page.initial748
dc.publisherWiley Periodicals
dc.relation.projectIDAGL2013-46448-C3-2-R
dc.rights.accessRightsrestricted access
dc.subject.cdu591.1
dc.subject.cdu612.82
dc.subject.cdu597.2/.5
dc.subject.keywordGhrelin O-acyltransferase
dc.subject.keywordghrelinergic system
dc.subject.keywordanatomical distribution
dc.subject.keywordRT-qPCR
dc.subject.keywordimmunohisto-chemistry
dc.subject.keywordfish
dc.subject.ucmBiología
dc.subject.ucmFisiología animal (Biología)
dc.subject.ucmNeurociencias (Biológicas)
dc.subject.ucmPeces
dc.subject.unesco24 Ciencias de la Vida
dc.subject.unesco2401.13 Fisiología Animal
dc.subject.unesco2490 Neurociencias
dc.titleBrain Mapping of Ghrelin O-Acyltransferase in Goldfish (Carassius Auratus): Novel Roles for the Ghrelinergic System in Fish?
dc.typejournal article
dc.volume.number299
dspace.entity.typePublication
relation.isAuthorOfPublication48071251-a236-4607-ab33-5332c1472628
relation.isAuthorOfPublication58727d30-dd58-40f9-962a-9bc051bfc74c
relation.isAuthorOfPublication.latestForDiscovery48071251-a236-4607-ab33-5332c1472628
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