Poly (ADP-ribose) polymerase 3 (PARP3), a potential repressor of telomerase activity
dc.contributor.author | Fernández-Marcelo, Tamara | |
dc.contributor.author | Frías, Cristina | |
dc.contributor.author | Pascua, Irene | |
dc.contributor.author | Juan Chocano, María Del Carmen De | |
dc.contributor.author | Head, Jacqueline | |
dc.contributor.author | Gómez, Ana | |
dc.contributor.author | Hernando Trancho, Florentino | |
dc.contributor.author | Jarabo Sarceda, José Ramón | |
dc.contributor.author | Díaz-Rubio García, Eduardo | |
dc.contributor.author | Torres García, Antonio José | |
dc.contributor.author | Rouleau, Michèle | |
dc.contributor.author | Benito De Las Heras, Manuel R. | |
dc.contributor.author | Iniesta, Pilar | |
dc.contributor.author | Iniesta Serrano, María Pilar | |
dc.date.accessioned | 2024-01-16T09:00:43Z | |
dc.date.available | 2024-01-16T09:00:43Z | |
dc.date.issued | 2014-02-15 | |
dc.description.abstract | Background Considering previous result in Non-Small Cell Lung Cancer (NSCLC), we investigated in human cancer cells the role of PARP3 in the regulation of telomerase activity. Methods We selected A549 (lung adenocarcinoma cell line) and Saos-2 (osteosarcoma cell line), with high and low telomerase activity levels, respectively. The first one was transfected using a plasmid construction containing a PARP3 sequence, whereas the Saos-2 cells were submitted to shRNA transfection to get PARP3 depletion. PARP3 expression on both cell systems was evaluated by real-time quantitative PCR and PARP3 protein levels, by Western-blot. Telomerase activity was determined by TRAP assay. Results In A549 cells, after PARP3 transient transfection, data obtained indicated that twenty-four hours after transfection, up to 100-fold increased gene expression levels were found in the transfected cells with pcDNA/GW-53/PARP3 in comparison to transfected cells with the empty vector. Moreover, 48 hours post-transfection, telomerase activity decreased around 33%, and around 27%, 96 hours post-transfection. Telomerase activity average ratio was 0.67 ± 0.05, and 0.73 ± 0.06, respectively, with significant differences. In Saos-2 cells, after shRNA-mediated PARP3 silencing, a 2.3-fold increase in telomerase activity was detected in relation to the control. Conclusion Our data indicated that, at least in some cancer cells, repression of PARP3 could be responsible for an increased telomerase activity, this fact contributing to telomere maintenance and, therefore, avoiding genome instability. | |
dc.description.department | Depto. de Bioquímica y Biología Molecular | |
dc.description.faculty | Fac. de Farmacia | |
dc.description.refereed | TRUE | |
dc.description.sponsorship | Fundación de Investigación Médica Mutua Madrileña | |
dc.description.sponsorship | Neumomadrid | |
dc.description.sponsorship | Banco Santander | |
dc.description.sponsorship | Universidad Complutense de Madrid | |
dc.description.sponsorship | Red Temática de Investigación Cooperativa en Cáncer | |
dc.description.status | pub | |
dc.identifier.citation | Fernández-Marcelo T, Frías C, Pascua I, De Juan C, Head J, Gómez A, et al. Poly (ADP-ribose) polymerase 3 (PARP3), a potential repressor of telomerase activity. J Exp Clin Cancer Res 2014;33:19. https://doi.org/10.1186/1756-9966-33-19. | |
dc.identifier.doi | 10.1186/1756-9966-33-19 | |
dc.identifier.essn | 1756-9966 | |
dc.identifier.officialurl | https://doi.org/10.1186/1756-9966-33-19 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14352/93262 | |
dc.journal.title | Journal of Experimental & Clinical Cancer Research | |
dc.language.iso | eng | |
dc.page.initial | 19 | |
dc.page.total | 8 | |
dc.publisher | BMC | |
dc.rights | Attribution 4.0 International | en |
dc.rights.accessRights | open access | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject.cdu | 616-006.04 | |
dc.subject.keyword | PARP3 | |
dc.subject.keyword | Telomerase | |
dc.subject.keyword | Lung cancer | |
dc.subject.keyword | Tumour cell lines | |
dc.subject.ucm | Ciencias Biomédicas | |
dc.subject.unesco | 24 Ciencias de la Vida | |
dc.title | Poly (ADP-ribose) polymerase 3 (PARP3), a potential repressor of telomerase activity | |
dc.type | journal article | |
dc.type.hasVersion | VoR | |
dc.volume.number | 33 | |
dspace.entity.type | Publication | |
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relation.isAuthorOfPublication.latestForDiscovery | 2a36a0b9-e416-47b7-a6fa-1dd706666732 |
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