Poly (ADP-ribose) polymerase 3 (PARP3), a potential repressor of telomerase activity

Simple item page
dc.contributor.authorFernández-Marcelo, Tamara
dc.contributor.authorFrías, Cristina
dc.contributor.authorPascua, Irene
dc.contributor.authorJuan Chocano, María Del Carmen De
dc.contributor.authorHead, Jacqueline
dc.contributor.authorGómez, Ana
dc.contributor.authorHernando Trancho, Florentino
dc.contributor.authorJarabo Sarceda, José Ramón
dc.contributor.authorDíaz-Rubio García, Eduardo
dc.contributor.authorTorres García, Antonio José
dc.contributor.authorRouleau, Michèle
dc.contributor.authorBenito De Las Heras, Manuel R.
dc.contributor.authorIniesta, Pilar
dc.contributor.authorIniesta Serrano, María Pilar
dc.date.accessioned2024-01-16T09:00:43Z
dc.date.available2024-01-16T09:00:43Z
dc.date.issued2014-02-15
dc.description.abstractBackground Considering previous result in Non-Small Cell Lung Cancer (NSCLC), we investigated in human cancer cells the role of PARP3 in the regulation of telomerase activity. Methods We selected A549 (lung adenocarcinoma cell line) and Saos-2 (osteosarcoma cell line), with high and low telomerase activity levels, respectively. The first one was transfected using a plasmid construction containing a PARP3 sequence, whereas the Saos-2 cells were submitted to shRNA transfection to get PARP3 depletion. PARP3 expression on both cell systems was evaluated by real-time quantitative PCR and PARP3 protein levels, by Western-blot. Telomerase activity was determined by TRAP assay. Results In A549 cells, after PARP3 transient transfection, data obtained indicated that twenty-four hours after transfection, up to 100-fold increased gene expression levels were found in the transfected cells with pcDNA/GW-53/PARP3 in comparison to transfected cells with the empty vector. Moreover, 48 hours post-transfection, telomerase activity decreased around 33%, and around 27%, 96 hours post-transfection. Telomerase activity average ratio was 0.67 ± 0.05, and 0.73 ± 0.06, respectively, with significant differences. In Saos-2 cells, after shRNA-mediated PARP3 silencing, a 2.3-fold increase in telomerase activity was detected in relation to the control. Conclusion Our data indicated that, at least in some cancer cells, repression of PARP3 could be responsible for an increased telomerase activity, this fact contributing to telomere maintenance and, therefore, avoiding genome instability.
dc.description.departmentDepto. de Bioquímica y Biología Molecular
dc.description.facultyFac. de Farmacia
dc.description.refereedTRUE
dc.description.sponsorshipFundación de Investigación Médica Mutua Madrileña
dc.description.sponsorshipNeumomadrid
dc.description.sponsorshipBanco Santander
dc.description.sponsorshipUniversidad Complutense de Madrid
dc.description.sponsorshipRed Temática de Investigación Cooperativa en Cáncer
dc.description.statuspub
dc.identifier.citationFernández-Marcelo T, Frías C, Pascua I, De Juan C, Head J, Gómez A, et al. Poly (ADP-ribose) polymerase 3 (PARP3), a potential repressor of telomerase activity. J Exp Clin Cancer Res 2014;33:19. https://doi.org/10.1186/1756-9966-33-19.
dc.identifier.doi10.1186/1756-9966-33-19
dc.identifier.essn1756-9966
dc.identifier.officialurlhttps://doi.org/10.1186/1756-9966-33-19
dc.identifier.urihttps://hdl.handle.net/20.500.14352/93262
dc.journal.titleJournal of Experimental & Clinical Cancer Research
dc.language.isoeng
dc.page.initial19
dc.page.total8
dc.publisherBMC
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu616-006.04
dc.subject.keywordPARP3
dc.subject.keywordTelomerase
dc.subject.keywordLung cancer
dc.subject.keywordTumour cell lines
dc.subject.ucmCiencias Biomédicas
dc.subject.unesco24 Ciencias de la Vida
dc.titlePoly (ADP-ribose) polymerase 3 (PARP3), a potential repressor of telomerase activity
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number33
dspace.entity.typePublication
relation.isAuthorOfPublication2a36a0b9-e416-47b7-a6fa-1dd706666732
relation.isAuthorOfPublication6ecae937-7e74-4f49-a22c-4a99a9103abd
relation.isAuthorOfPublicationf768a316-05d7-4b2d-858d-9b8be4686e1e
relation.isAuthorOfPublicationb60fef8b-3d27-45a9-9f69-0cd05cc57a2f
relation.isAuthorOfPublication790390e8-2a0b-4dca-9996-3e85d11acad7
relation.isAuthorOfPublication6a240551-5797-4599-8d91-76bc38fecf8d
relation.isAuthorOfPublicationfeb931a4-70c5-4e00-a151-12d4f2573b2c
relation.isAuthorOfPublication.latestForDiscovery2a36a0b9-e416-47b7-a6fa-1dd706666732
Download
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
1756-9966-33-19.pdf
Size:
2.31 MB
Format:
Adobe Portable Document Format
downoload Download
Collections
Artículos