The expression of aminoglycoside resistance genes in integron cassettes is not controlled by riboswitches

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dc.contributor.authorHipólito, Alberto
dc.contributor.authorGarcía Pastor, Lucía
dc.contributor.authorTrigo da Roza, Filipa
dc.contributor.authorKieffer, Nicolas
dc.contributor.authorVergara, Ester
dc.contributor.authorJové, Thomas
dc.contributor.authorEscudero García-Calderón, José Antonio
dc.contributor.authorBlanco Torres, Paula
dc.contributor.authorÁlvarez Sánchez, Julio
dc.date.accessioned2024-01-22T19:55:22Z
dc.date.available2024-01-22T19:55:22Z
dc.date.issued2022-08-26
dc.description.abstractRegulation of gene expression is a key factor influencing the success of antimicrobial resistance determinants. A variety of determinants conferring resistance against aminoglycosides (Ag) are commonly found in clinically relevant bacteria, but whether their expression is regulated or not is controversial. The expression of several Ag resistance genes has been reported to be controlled by a riboswitch mechanism encoded in a conserved sequence. Yet this sequence corresponds to the integration site of an integron, a genetic platform that recruits genes of different functions, making the presence of such a riboswitch counterintuitive. We provide, for the first time, experimental evidence against the existence of such Ag-sensing riboswitch. We first tried to reproduce the induction of the well characterized aacA5 gene using its native genetic environment, but were unsuccessful. We then broadened our approach and analyzed the inducibility of all AgR genes encoded in integrons against a variety of antibiotics. We could not observe biologically relevant induction rates for any gene in the presence of several aminoglycosides. Instead, unrelated antibiotics produced mild but consistently higher increases in expression, that were the result of pleiotropic effects. Our findings rule out the riboswitch control of aminoglycoside resistance genes in integrons.
dc.description.departmentDepto. de Sanidad Animal
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.statuspub
dc.identifier.doi10.1093/nar/gkac662
dc.identifier.essn1362-4962
dc.identifier.issn0305-1048
dc.identifier.officialurlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9410878/
dc.identifier.pmid35947699
dc.identifier.urihttps://hdl.handle.net/20.500.14352/94566
dc.issue.number15
dc.journal.titleNucleic Acids Research
dc.language.isoeng
dc.page.final8579
dc.page.initial8566
dc.publisherOUP
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.ucmCiencias Biomédicas
dc.subject.unesco24 Ciencias de la Vida
dc.titleThe expression of aminoglycoside resistance genes in integron cassettes is not controlled by riboswitches
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number50
dspace.entity.typePublication
relation.isAuthorOfPublicationf2840ea1-2146-4244-babd-79c986a18986
relation.isAuthorOfPublicationec7d4d32-3e8c-45cc-a2a2-87d8ac4f9053
relation.isAuthorOfPublication7a0cfc93-a3f1-45bf-b529-403f216cf8f7
relation.isAuthorOfPublication.latestForDiscoveryec7d4d32-3e8c-45cc-a2a2-87d8ac4f9053
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