A Metabolomics Study by 1H HRMAS NMR: From Sheep Milk to a Pressed-Curd Cheese: A Proof of Concept
dc.contributor.author | Castejón Ferrer, David | |
dc.contributor.author | Segura Plaza, José Francisco | |
dc.contributor.author | Cruz Díaz, Karen Paola | |
dc.contributor.author | Remiro Yagüe, Víctor | |
dc.contributor.author | Fernández Valle, María Encarnación | |
dc.contributor.author | Romero De Ávila Hidalgo, María Dolores | |
dc.contributor.author | Villa Valverde, Palmira | |
dc.contributor.author | Cambero Rodríguez, María Isabel | |
dc.date.accessioned | 2024-07-10T08:49:50Z | |
dc.date.available | 2024-07-10T08:49:50Z | |
dc.date.issued | 2024-04-13 | |
dc.description | This article belongs to the Collection "Analytical and Applied Chemistry: the challenges and opportunities for growth in the 21st century" | |
dc.description | Autorizado por la editorial: portada, índice y primeras páginas de la obra. | |
dc.description.abstract | For the first time, High-Resolution Magic Angle Nuclear Magnetic Resonance spectroscopy (NMR-HRMAS) was applied to directly identify specific metabolites from a Spanish raw ewe’s milk and enzymatic coagulation pressed-curd cheese (Protected Geographical Indication: Castellano) manufactured by two procedures (traditional/artisanal vs. industrial) and including the ewe’s raw milk. The NMR parameters were optimized to study the complex matrixes of this type of cheese. In addition, conventional overcrowded 1H-NMR-HRMAS spectra were selectively simplified by a Carr–Purcell–Meiboom–Gill (CPMG) sequence or a stimulated echo pulse sequence by bipolar gradients (DIFF), thus modulating spin–spin relaxation times and diffusion of molecular components, respectively. 1H-NMR-HRMAS spectroscopy displayed important information about cheese metabolites, which can be associated with different manufacturing processes (industrial vs. traditional) and ripening times (from 2 to 90 days). These results support that this spectroscopy is a useful technique to monitor the ripening process, from raw milk to commercial ripened cheese, using a minimum intact sample, implying the absence of time-consuming sample pretreatments. | |
dc.description.department | Sección Deptal. de Farmacia Galénica y Tecnología Alimentaria (Veterinaria) | |
dc.description.faculty | Fac. de Veterinaria | |
dc.description.refereed | TRUE | |
dc.description.sponsorship | Ministerio de Ciencia, Innovación y Universidades (España) | |
dc.description.status | pub | |
dc.identifier.citation | Castejón, D.; Segura, J.; Cruz-Díaz, K.P.; Remiro, V.; Fernández-Valle, M.E.; Romero de Ávila, M.D.; Villa, P.; Cambero, M.I. A Metabolomics Study by 1H HRMAS NMR: From Sheep Milk to a PressedCurd Cheese: A Proof of Concept. Analytica 2024, 5, 170–186. https:// doi.org/10.3390/analytica5020011 | |
dc.identifier.doi | 10.3390/analytica5020011 | |
dc.identifier.essn | 2673-4532 | |
dc.identifier.officialurl | https://doi.org/10.3390/analytica5020011 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14352/105894 | |
dc.issue.number | 2 | |
dc.journal.title | Analytica | |
dc.language.iso | eng | |
dc.page.final | 186 | |
dc.page.initial | 170 | |
dc.publisher | MDPI | |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-107542RB-C22/ES/METODOLOGIAS DE RESONANCIA MAGNETICA NUCLEAR (RMN) COMO HERRAMIENTAS DE AUTOMATIZACION Y CONTROL EN EL SECTOR CARNICO/ | |
dc.rights | Attribution 4.0 International | en |
dc.rights.accessRights | open access | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject.cdu | 641 | |
dc.subject.keyword | Foodomics | |
dc.subject.keyword | Metabolomics | |
dc.subject.keyword | HRMAS-NMR | |
dc.subject.keyword | Castellano cheese | |
dc.subject.keyword | Fatty acid | |
dc.subject.keyword | Amino acid | |
dc.subject.ucm | Tecnología de los alimentos | |
dc.subject.unesco | 3309 Tecnología de Los Alimentos | |
dc.title | A Metabolomics Study by 1H HRMAS NMR: From Sheep Milk to a Pressed-Curd Cheese: A Proof of Concept | |
dc.type | journal article | |
dc.type.hasVersion | VoR | |
dc.volume.number | 5 | |
dspace.entity.type | Publication | |
relation.isAuthorOfPublication | 6a7ae18a-be91-4154-ba36-03c6aec972c2 | |
relation.isAuthorOfPublication | 7555003c-f428-4969-9333-941b580f4d86 | |
relation.isAuthorOfPublication | 22a0098d-5a97-46ac-8dc2-077394506f2e | |
relation.isAuthorOfPublication.latestForDiscovery | 6a7ae18a-be91-4154-ba36-03c6aec972c2 |
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