Epitope Selection for Fighting Visceral Leishmaniosis: Not All Peptides Function the Same Way

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dc.contributor.authorÁlvarez-Campos, Daniel
dc.contributor.authorMartínez Rodrigo, Abel
dc.contributor.authorMas Zubiri, Alicia
dc.contributor.authorOrden Gutiérrez, José Antonio
dc.contributor.authorDomínguez Bernal, Gustavo Ramón
dc.contributor.authorCarrión Herrero, Francisco Javier
dc.date.accessioned2024-01-11T14:29:01Z
dc.date.available2024-01-11T14:29:01Z
dc.date.issued2020-07-01
dc.description.abstractVisceral leishmaniosis (VL) caused by Leishmania infantum is a disease with an increasing prevalence worldwide. Treatments are expensive, toxic, and ineffective. Therefore, vaccination seems to be a promising approach to control VL. Peptide-based vaccination is a useful method due to its stability, absence of local side effects, and ease of scaling up. In this context, bioinformatics seems to facilitate the use of peptides, as this analysis can predict high binding affinity epitopes to MHC class I and II molecules of different species. We have recently reported the use of HisAK70 DNA immunization in mice to induce a resistant phenotype against L. major, L. infantum, and L. amazonensis infections. In the present study, we used bioinformatics tools to select promising multiepitope peptides (HisDTC and AK) from the polyprotein encoded in the HisAK70 DNA to evaluate their immunogenicity in the murine model of VL by L. infantum. Our results revealed that both multiepitope peptides were able to induce the control of VL in mice. Furthermore, HisDTC was able to induce a better cell-mediated immune response in terms of reduced parasite burden, protective cytokine profile, leishmanicidal enzyme modulation, and specific IgG2a isotype production in immunized mice, before and after infectious challenge. Overall, this study indicates that the HisDTC chimera may be considered a satisfactory tool to control VL because it is able to activate a potent CD4+ and CD8+ T-cell protective immune responses.
dc.description.departmentDepto. de Sanidad Animal
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipFECYT
dc.description.sponsorshipUCM-SANTANDER
dc.description.sponsorshipCOMUNIDAD DE MADRID
dc.description.statuspub
dc.identifier.citationMolina-Rueda F, Fernández-González P, Cuesta-Gómez A, Koutsou A, Carratalá-Tejada M, Miangolarra JC. Test-Retest Reliability of a Conventional Gait Model for Registering Joint Angles during Initial Contact and Toe-Off in Healthy Subjects. Int J Environ Res Public Health. 2021 Feb 15;3(18):1343-52
dc.identifier.doi10.3390/vaccines8030352
dc.identifier.issn2076-393X
dc.identifier.officialurlhttps://www.mdpi.com/2076-393X/8/3/352
dc.identifier.urihttps://hdl.handle.net/20.500.14352/92572
dc.issue.number3
dc.journal.titleVaccines
dc.language.isoeng
dc.page.final370
dc.page.initial352
dc.publisherMDPI
dc.relation.projectIDPRECIPITA (PR22I-FECYT
dc.relation.projectIDUCM-Santander (Grant PR75/18-21558)
dc.relation.projectIDPLATESA2-CM (S2018/BAA-4370)
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu636.09:615.37
dc.subject.keywordLeishmania infantum
dc.subject.keywordIn silico analysis
dc.subject.keywordEpitope prediction
dc.subject.keywordMultiepitope-based vaccine
dc.subject.keywordLeishmaniosis
dc.subject.keywordHisDTE
dc.subject.ucmCiencias Biomédicas
dc.subject.unesco32 Ciencias Médicas
dc.titleEpitope Selection for Fighting Visceral Leishmaniosis: Not All Peptides Function the Same Way
dc.typejournal article
dc.type.hasVersionAM
dc.volume.number8
dspace.entity.typePublication
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