Expansion microscopy applied to mono- and dual-species biofilms

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dc.contributor.authorValdivieso González, David
dc.contributor.authorJara Pérez, Josué
dc.contributor.authorAlmendro Vedia, Víctor Galileo
dc.contributor.authorOrgaz Martín, Belén
dc.contributor.authorLópez Montero, Iván
dc.date.accessioned2024-05-20T16:46:33Z
dc.date.available2024-05-20T16:46:33Z
dc.date.issued2023-12-05
dc.description.abstractExpansion microscopy (ExM) is a new super-resolution technique based on embedding the biological sample within a hydrogel and its physical expansion after swelling. This allows increasing its size by several times while preserving its structural details. Applied to prokaryotic cells, ExM requires digestion steps for efficient expansion as bacteria are surrounded by a rigid cell wall. Furthermore, bacteria can live in social groups forming biofilms, where cells are protected from environmental stresses by a self-produced matrix. The extracellular matrix represents an additional impenetrable barrier for ExM. Here we optimize the current protocols of ExM and apply them to mono- and dual-species biofilms formed by clinical isolates of Limosilactobacillus reuteri, Enterococcus faecalis, Serratia marcescens and Staphylococcus aureus. Using scanning electron microscopy for comparison, our results demonstrate that embedded bacteria expanded 3-fold. Moreover, ExM allowed visualizing the three-dimensional architecture of the biofilm and identifying the distribution of different microbial species and their interactions. We also detected the presence of the extracellular matrix after expansion with a specific stain of the polysaccharide component. The potential applications of ExM in biofilms will improve our understanding of these complex communities and have far-reaching implications for industrial and clinical research.
dc.description.departmentDepto. de Química Física
dc.description.departmentSección Dptal. de Nutrición y Ciencia de los Alimentos (Veterinaria)
dc.description.departmentSección Deptal. de Farmacia Galénica y Tecnología Alimentaria (Veterinaria)
dc.description.facultyFac. de Veterinaria
dc.description.facultyFac. de Ciencias Químicas
dc.description.facultyInstituto Pluridisciplinar (IP)
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia, Innovación y Universidades (España)
dc.description.sponsorshipComunidad de Madrid
dc.description.sponsorshipUniversidad Complutense de Madrid
dc.description.statuspub
dc.identifier.citationValdivieso González, D., Jara, J., Almendro-Vedia, V.G. et al. Expansion microscopy applied to mono- and dual-species biofilms. npj Biofilms Microbiomes 9, 92 (2023). https://doi.org/10.1038/s41522-023-00460-x
dc.identifier.doi10.1038/s41522-023-00460-x
dc.identifier.essn2055-5008
dc.identifier.officialurlhttps://doi.org/10.1038/s41522-023-00460-x
dc.identifier.pmid38049404
dc.identifier.relatedurlhttps://pubmed.ncbi.nlm.nih.gov/38049404/
dc.identifier.urihttps://hdl.handle.net/20.500.14352/104235
dc.issue.number92
dc.journal.titleBiofilms and Microbiomes
dc.language.isoeng
dc.publisherNature Research
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-097903-B-I00/ES/PROPIEDADES EMERGENTES DE NANOROTORES BIOLOGICOAS EN MEDIOS VISCOELASTICOS/
dc.relation.projectIDPID2021-125024NB-C22
dc.relation.projectIDPID2019-105606RB-I00
dc.relation.projectIDPR27/21-030 (to V.G.A.-V.)
dc.relation.projectIDCT103/19/PEJ2019-AI/IND-13687
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu641.1
dc.subject.ucmTecnología de los alimentos
dc.subject.unesco3309 Tecnología de Los Alimentos
dc.titleExpansion microscopy applied to mono- and dual-species biofilms
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number9(1)
dspace.entity.typePublication
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