Simultaneous determination of CXCL7 chemokine and MMP3 metalloproteinase as biomarkers for rheumatoid arthritis

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dc.contributor.authorGuerrero Irigoyen, Sara
dc.contributor.authorSánchez Tirado, Esther
dc.contributor.authorAgüí Chicharro, María Lourdes
dc.contributor.authorGonzález Cortés, Araceli
dc.contributor.authorYáñez-Sedeño Orive, Paloma
dc.contributor.authorPingarrón Carrazón, José Manuel
dc.date.accessioned2024-01-11T18:36:01Z
dc.date.available2024-01-11T18:36:01Z
dc.date.issued2021-07-09
dc.description.abstractThis paper reports the preparation of the first dual electrochemical immunosensor for the simultaneous determination of the CXCL7 chemokine and the MMP3 metalloproteinase as relevant biomarkers for the better diagnosis and monitoring of rheumatoid arthritis derived from the multiple biomarkers measurement. The developed immunosensor involves the use of carboxylated magnetic beads (MBs) and dual screen-printed carbon electrodes (SPdCEs). Sandwich-type configurations implied the covalent immobilization of specific anti-CXCL7 (cAb1) or anti-MMP3 (cAb2) capture antibodies onto MBs and the use of biotinylated detection antibodies with further labelling with HRP-Strept conjugates. The resulting MBS bioconjugates were magnetically captured on the respective working electrode of the SPdCE and the determination of the antigens was accomplished by measuring the amperometric responses of H2O2 mediated by hydroquinone (HQ) at a potential value of −0.20 V. The dual immunosensor provided calibration plots with linear ranges between 1 and 75 ng mL−1 (CXCL7) (R2 = 0.997) and from 2.0 to 2000 pg mL−1 (MMP3) (R2 = 0.998) with detection limits of 0.8 ng mL−1 and 1.2 pg mL−1, respectively. The assay took 2 h 20 min for the simultaneous determination of both biomarkers. The dual immunosensor was successfully applied to the analysis of human serum from positive and negative RA patients.
dc.description.departmentDepto. de Química Analítica
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia, Innovación y Universidades
dc.description.sponsorshipComunidad de Madrid
dc.description.statuspub
dc.identifier.citationGuerrero S, Sánchez-Tirado E, Agüí L, González-Cortés A, Yáñez-Sedeño P, Pingarrón JM. Simultaneous determination of CXCL7 chemokine and MMP3 metalloproteinase as biomarkers for rheumatoid arthritis. Talanta. 2021 Jul 9;234: 122705 - 122712.
dc.identifier.doi10.1016/j.talanta.2021.122705
dc.identifier.issn0039-9140
dc.identifier.officialurlhttps://www.sciencedirect.com/science/article/pii/S0039914021006263
dc.identifier.urihttps://hdl.handle.net/20.500.14352/92648
dc.journal.titleTalanta
dc.language.isoeng
dc.page.final122712
dc.page.initial122705
dc.publisherElsevier
dc.relation.projectIDRTI2018-096135-B-100
dc.relation.projectIDS2018/NMT-4349
dc.rightsAttribution-NonCommercial 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subject.cdu543
dc.subject.cdu544.6:543.25
dc.subject.cdu543.25:544.6
dc.subject.keywordDual immunosensor
dc.subject.keywordChemokines
dc.subject.keywordMetalloproteinases
dc.subject.keywordCXCL7
dc.subject.keywordMMP3
dc.subject.keywordAutoimmune diseases
dc.subject.keywordRheumatoid arthritis
dc.subject.keywordBiomarkers
dc.subject.keywordElectrochemical immunosensor
dc.subject.keywordSerum
dc.subject.ucmQuímica analítica (Química)
dc.subject.unesco2301.04 Análisis Electroquímico
dc.titleSimultaneous determination of CXCL7 chemokine and MMP3 metalloproteinase as biomarkers for rheumatoid arthritis
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number234
dspace.entity.typePublication
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