RNA sequencing of mesenchymal stem cells reveals a blocking of differentiation and immunomodulatory activities under inflammatory conditions in rheumatoid arthritis patients

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dc.contributor.authorLamas, José Ramón
dc.contributor.authorFernández Gutiérrez, Benjamín
dc.contributor.authorMucientes, Arkaitz
dc.contributor.authorMarco Martínez, Fernando
dc.contributor.authorLópiz Morales, María Yaiza
dc.contributor.authorJover Jover, Juan Ángel
dc.contributor.authorAbasolo, Lydia
dc.contributor.authorRodríguez Rodríguez, Luis
dc.date.accessioned2024-08-05T11:08:41Z
dc.date.available2024-08-05T11:08:41Z
dc.date.issued2019-05-06
dc.description.abstractIntroduction: Mesenchymal stem cells (MSCs) have the ability to differentiate into different types of cells of the mesenchymal lineage, such as osteocytes, chondrocytes, and adipocytes. It is also known that under inflammatory stimuli or in the appropriate experimental conditions, they can also act as regulators of inflammation. Thus, in addition to their regenerating potential, their interest has been extended to their possible use in cell therapy strategies for treatment of immune disorders. Objective: To analyze, by RNA-seq analysis, the transcriptome profiling of allogenic MSCs under RA lymphocyte activation. Methods: We identified the differentially expressed genes in bone marrow mesenchymal stem cells after exposure to an inflammatory environment. The transcriptome profiling was evaluated by means of the precise measurement of transcripts provided by the RNA-Seq technology. Results: Our results evidenced the existence of blocking of both regenerative (differentiation) and immunomodulatory phenotypes under inflammatory conditions characterized by an upregulation of genes involved in immune processes and a simultaneous downregulation of genes mainly involved in regenerative or cell differentiation functions. Conclusions: We conclude that the two main functions of MSCs (immunomodulation and differentiation) are blocked, at least while the inflammation is being resolved. Inflammation, at least partially mediated by gamma-interferon, drives MSCs to a cellular distress adopting a defensive state. This knowledge could be of particular interest in cases where the damage to be repaired has an important immune-mediated component.
dc.description.departmentDepto. de Cirugía
dc.description.facultyFac. de Medicina
dc.description.refereedTRUE
dc.description.sponsorshipEuropean Commission
dc.description.sponsorshipInstituto de Salud Carlos III (España)
dc.description.sponsorshipMinisterio de Ciencia, Innovación y Universidades (España)
dc.description.statuspub
dc.identifier.citationLamas JR, Fernandez-Gutierrez B, Mucientes A, Marco F, Lopiz Y, Jover JA, Abasolo L, Rodríguez-Rodríguez L. RNA sequencing of mesenchymal stem cells reveals a blocking of differentiation and immunomodulatory activities under inflammatory conditions in rheumatoid arthritis patients. Arthritis Res Ther. 2019 May 6;21(1):112. doi: 10.1186/s13075-019-1894-y. PMID: 31060598; PMCID: PMC6501285.
dc.identifier.doi10.1186/s13075-019-1894-y
dc.identifier.essn1478-6362
dc.identifier.issn1478-6354
dc.identifier.officialurlhttps://doi.org/10.1186/s13075-019-1894-y
dc.identifier.relatedurlhttps://arthritis-research.biomedcentral.com/articles/10.1186/s13075-019-1894-y
dc.identifier.urihttps://hdl.handle.net/20.500.14352/107385
dc.issue.number112
dc.journal.titleArthritis Research & Therapy
dc.language.isoeng
dc.publisherBMC
dc.relation.projectIDinfo:eu-repo/grantAgreement/ISCIII/RIER/16/0012/0004
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//PI13%2F01863/ES/Estudio del comportamiento funcional en scaffolds biocerámicos de células madres mesenquimales obtenidas de distintos orígenes/
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.keywordImmunomodulation
dc.subject.keywordMesenchymal stem cells
dc.subject.keywordRNA-seq
dc.subject.keywordRheumatoid arthritis
dc.subject.ucmMedicina
dc.subject.unesco32 Ciencias Médicas
dc.titleRNA sequencing of mesenchymal stem cells reveals a blocking of differentiation and immunomodulatory activities under inflammatory conditions in rheumatoid arthritis patients
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number21
dspace.entity.typePublication
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