Proteomic characterization of EPCs and CECs “in vivo” from acute coronary syndrome patients and control subjects

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dc.contributor.authorMourino-Alvarez, L.
dc.contributor.authorCalvo, E.
dc.contributor.authorMoreu, J.
dc.contributor.authorPadial, L.R.
dc.contributor.authorLópez, J.A.
dc.contributor.authorBarderas, M.G.
dc.contributor.authorGil Dones, Félix
dc.date.accessioned2024-01-23T08:26:38Z
dc.date.available2024-01-23T08:26:38Z
dc.date.issued2013-04
dc.description.abstractBackground: Circulating endothelial cells (CECs) and endothelial progenitor cells (EPCs) represent two scarce blood populations that are thought to play important roles in tissue vascularization. They have also been proposed as potential markers for more than a dozen pathologies. Moreover, EPCs have arisen as a new therapeutic option for cardiovascular disease. However nowadays there is certain controversy about their roles and a better understanding of EPC biology is required to develop new strategies for forthcoming therapies. Methods: Flow cytometry analysis was performed on freshly isolated mononuclear cells from control subjects and Acute Coronary Syndrome (ACS) patients. EPCs and CECs for both groups were isolated and quantified. Statistical analyses were performed to test the potential biomarker usefulness of both populations in ACS together with the first “in vivo” proteomic characterizations of these populations. Results: Our results do not show statistical differences in the quantification of CECs and EPCs in control subjects and ACS patients. The proteomic characterization allowed us to identify 673 proteins associated to CECs (389 in controls and 462 in ACS patients), and another 502 proteins in EPCs (350 in controls and 274 in ACS patients).
dc.description.departmentDepto. de Genética, Fisiología y Microbiología
dc.description.facultyFac. de Ciencias Biológicas
dc.description.refereedTRUE
dc.description.sponsorshipFondo de Investigación Sanitaria de Castilla la Mancha
dc.description.statuspub
dc.identifier.doi10.1016/j.bbagen.2012.12.014
dc.identifier.issn0304-4165
dc.identifier.officialurlhttps://www.sciencedirect.com/science/article/pii/S0304416512003753?via%3Dihub
dc.identifier.urihttps://hdl.handle.net/20.500.14352/94591
dc.issue.number4
dc.journal.titleBiochimica et Biophysica Acta (BBA) - General Subjects
dc.language.isoeng
dc.page.final3053
dc.page.initial3030
dc.publisherScienceDirect
dc.relation.projectID(FISCAM,PI2008/28andPI2008/08)
dc.rights.accessRightsrestricted access
dc.subject.cdu577.112
dc.subject.cdu616.12
dc.subject.keywordEPCs
dc.subject.keywordCECs
dc.subject.keywordBiomarker
dc.subject.keywordProteomics
dc.subject.keywordAcute coronary syndrome
dc.subject.ucmBioquímica (Biología)
dc.subject.ucmCardiología
dc.subject.unesco2403 Bioquímica
dc.subject.unesco3205.01 Cardiología
dc.titleProteomic characterization of EPCs and CECs “in vivo” from acute coronary syndrome patients and control subjects
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number1830
dspace.entity.typePublication
relation.isAuthorOfPublication0827e638-921a-4475-9a48-b859587719c5
relation.isAuthorOfPublication.latestForDiscovery0827e638-921a-4475-9a48-b859587719c5
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