Draft Genome Sequence of Lactococcus lactis Subsp. cremoris WA2-67: A Promising Nisin-Producing Probiotic Strain Isolated from the Rearing Environment of a Spanish Rainbow Trout (Oncorhynchus mykiss, Walbaum) Farm

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dc.contributor.authorFeito Hermida, Javier
dc.contributor.authorContente, Diogo
dc.contributor.authorPonce Alonso, Manuel
dc.contributor.authorDíaz Formoso, Lara
dc.contributor.authorAraújo, Carlos
dc.contributor.authorPeña Vidal, Nuria
dc.contributor.authorBorrero Del Pino, Juan
dc.contributor.authorGómez Sala, Beatriz
dc.contributor.authorCampo, Rosa del
dc.contributor.authorMuñoz Atienza, Estefanía
dc.contributor.authorHernández Cruza, Pablo Elpidio
dc.contributor.authorCintas Izarra, Luis Miguel
dc.date.accessioned2023-06-22T12:27:10Z
dc.date.available2023-06-22T12:27:10Z
dc.date.issued2022-02-28
dc.description.abstractProbiotics are a viable alternative to traditional chemotherapy agents to control infectious diseases in aquaculture. In this regard, Lactococcus lactis subsp. cremoris WA2-67 has previously demonstrated several probiotic features, such as a strong antimicrobial activity against ichthyopathogens, survival in freshwater, resistance to fish bile and low pH, and hydrophobicity. The aim of this manuscript is an in silico analysis of the whole-genome sequence (WGS) of this strain to gain deeper insights into its probiotic properties and their genetic basis. Genomic DNA was purified, and libraries prepared for Illumina sequencing. After trimming and assembly, resulting contigs were subjected to bioinformatic analyses. The draft genome of L. cremoris WA2-67 consists of 30 contigs (2,573,139 bp), and a total number of 2493 coding DNA sequences (CDSs). Via in silico analysis, the bacteriocinogenic genetic clusters encoding the lantibiotic nisin Z (NisZ) and two new bacteriocins were identified, in addition to several probiotic traits, such as the production of vitamins, amino acids, adhesion/aggregation, and stress resistance factors, as well as the absence of transferable antibiotic resistance determinants and genes encoding detrimental enzymatic activities and virulence factors. These results unveil diverse beneficial properties that support the use of L. cremoris WA2-67 as a probiotic for aquaculture.
dc.description.departmentSección Deptal. de Farmacia Galénica y Tecnología Alimentaria (Veterinaria)
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia e Innovación (MICINN)
dc.description.sponsorshipUniversidad Complutense de Madrid/Comunidad de Madrid
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/75115
dc.identifier.doi10.3390/microorganisms10030521
dc.identifier.issn2076-2607
dc.identifier.officialurlhttps://doi.org/10.3390/microorganisms10030521
dc.identifier.relatedurlhttps://www.mdpi.com/2076-2607/10/3/521/htm
dc.identifier.urihttps://hdl.handle.net/20.500.14352/72514
dc.issue.number3
dc.journal.titleMicroorganisms
dc.language.isoeng
dc.page.initial521
dc.publisherMPDI
dc.relation.projectIDRTI2018-094907-B-I00
dc.relation.projectID2018-T1/BIO-10158
dc.rightsAtribución 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/es/
dc.subject.keywordaquaculture
dc.subject.keywordprobiotics
dc.subject.keywordlactic acid bacteria
dc.subject.keywordbacteriocins
dc.subject.keywordnisin Z
dc.subject.ucmGenética
dc.subject.unesco2409 Genética
dc.titleDraft Genome Sequence of Lactococcus lactis Subsp. cremoris WA2-67: A Promising Nisin-Producing Probiotic Strain Isolated from the Rearing Environment of a Spanish Rainbow Trout (Oncorhynchus mykiss, Walbaum) Farm
dc.typejournal article
dc.volume.number10
dspace.entity.typePublication
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relation.isAuthorOfPublication.latestForDiscovery48922a93-481a-488b-b69d-d70831dea16c
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