A Reliable and Standardizable Differential PCR and qPCR Methodology Assesses HER2 Gene Amplification in Gastric Cancer

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dc.contributor.authorJuárez Martín-Delgado, Ignacio
dc.contributor.authorToro Fernandez, Juan Francisco
dc.contributor.authorVaquero Yuste, Christian
dc.contributor.authorMolina Alejandre, Marta
dc.contributor.authorLasa, Inmaculada
dc.contributor.authorGomez, Remedios
dc.contributor.authorLopez, Adela
dc.contributor.authorMartín Villa, José Manuel
dc.contributor.authorGutierrez, Alberto
dc.date.accessioned2024-01-11T11:39:08Z
dc.date.available2024-01-11T11:39:08Z
dc.date.issued2021-06-10
dc.description.abstractWe have applied two PCR techniques, differential PCR (diffPCR) and qPCR for the identification of HER2 gene amplifications in genomic DNA of tumor and distal gastric samples from patients with gastric cancer. The diffPCR technique consists of the simultaneous amplification of the HER2 gene and a housekeeping gene by conventional PCR and the densitometric analysis of the bands obtained. We established a cut-off point based on the mean and standard deviation analyzing the DNA of 30 gastric tissues from patients undergoing non-cancer gastrectomy. diffPCR and qPCR yielded consistent results. HER2-overexpression was detected in 25% of patients and was further confirmed by immunohistochemistry and immunofluorescence. The approaches herein described may serve as complementary and reliable methods to assess HER2 amplification.
dc.description.departmentDepto. de Inmunología, Oftalmología y ORL
dc.description.facultyFac. de Medicina
dc.description.refereedTRUE
dc.description.sponsorshipUnión Europea
dc.description.sponsorshipInstituto de Salud Carlos III
dc.description.statuspub
dc.identifier.citationJuarez, I.; Toro-Fernandez, J.F.; Vaquero-Yuste, C.; Molina-Alejandre, M.; Lasa, I.; Gomez, R.; Lopez, A.; Martin-Villa, J.M.; Gutierrez, A. A Reliable and Standardizable Differential PCR and qPCR Methodology Assesses HER2 Gene Amplification in Gastric Cancer. Biology 2021, 10, 516. https://doi.org/10.3390/biology10060516
dc.identifier.doi10.3390/biology10060516
dc.identifier.issn2079-7737
dc.identifier.officialurlhttps://www.mdpi.com/2079-7737/10/6/516
dc.identifier.urihttps://hdl.handle.net/20.500.14352/92509
dc.issue.number516
dc.journal.titleBiology
dc.language.isoeng
dc.publisherMDPI
dc.relation.projectIDFEDER
dc.relation.projectIDinfo:eu-repo/grantAgreement/ISCIII/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016 (ISCIII)/PI18%2F00626/ES/HLA-G: CANCER GASTRICO Y SU EVOLUCION/
dc.relation.projectIDPI12/01663
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu577.2
dc.subject.keywordHER2
dc.subject.keywordGastric cancer
dc.subject.keywordCancer
dc.subject.keywordPCR
dc.subject.keywordImmunotherapy
dc.subject.ucmBiología molecular (Biología)
dc.subject.unesco2415 Biología Molecular
dc.titleA Reliable and Standardizable Differential PCR and qPCR Methodology Assesses HER2 Gene Amplification in Gastric Cancer
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number10
dspace.entity.typePublication
relation.isAuthorOfPublication0894d58b-fe71-42aa-ad0a-19305823daa1
relation.isAuthorOfPublicationd6c35711-8ed5-412f-8345-8b8e3869353a
relation.isAuthorOfPublication.latestForDiscovery0894d58b-fe71-42aa-ad0a-19305823daa1
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