Dimerization of the pulmonary surfactant protein C in a membrane environment
dc.contributor.author | Korolainen, Hanna | |
dc.contributor.author | Lolicato, Fabio | |
dc.contributor.author | Enkavi, Giray | |
dc.contributor.author | Pérez Gil, Jesús | |
dc.contributor.author | Kulig, Waldemar | |
dc.contributor.author | Vattulainen, Ilpo | |
dc.date.accessioned | 2023-06-22T10:53:48Z | |
dc.date.available | 2023-06-22T10:53:48Z | |
dc.date.issued | 2022-04-27 | |
dc.description.abstract | Surfactant protein C (SP-C) has several functions in pulmonary surfactant. These include the transfer of lipids between different membrane structures, a role in surfactant recycling and homeostasis, and involvement in modulation of the innate defense system. Despite these important functions, the structures of functional SP-C complexes have remained unclear. SP-C is known to exist as a primarily α-helical structure with an apparently unstructured N-terminal region, yet there is recent evidence that the functions of SP-C could be associated with the formation of SP-C dimers and higher oligomers. In this work, we used molecular dynamics simulations, two-dimensional umbrella sampling, and well-tempered metadynamics to study the details of SP-C dimerization. The results suggest that SP-C dimerizes in pulmonary surfactant membranes, forming dimers of different topologies. The simulations identified a dimerization motif region V21xxxVxxxGxxxM33 that is much larger than the putative A30xxxG34 motif that is commonly assumed to control the dimerization of some α-helical transmembrane domains. The results provide a stronger basis for elucidating how SP-C functions in concert with other surfactant proteins. | |
dc.description.department | Sección Deptal. de Bioquímica y Biología Molecular (Biológicas) | |
dc.description.faculty | Fac. de Ciencias Biológicas | |
dc.description.refereed | TRUE | |
dc.description.sponsorship | Ministerio de Ciencia e Innovación (MICINN) | |
dc.description.sponsorship | Comunidad de Madrid | |
dc.description.sponsorship | Academy of Finland | |
dc.description.sponsorship | Doctoral Programme in Materials Research and Nanosciences (MATRENA) | |
dc.description.status | pub | |
dc.eprint.id | https://eprints.ucm.es/id/eprint/74033 | |
dc.identifier.doi | 10.1371/journal.pone.0267155 | |
dc.identifier.issn | 1932-6203 | |
dc.identifier.officialurl | https://doi.org/10.1371/journal.pone.0267155 | |
dc.identifier.relatedurl | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0267155 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14352/71853 | |
dc.issue.number | 4 | |
dc.journal.title | PLoS ONE | |
dc.language.iso | eng | |
dc.page.final | 15 | |
dc.page.initial | 1 | |
dc.publisher | Public Library Science | |
dc.relation.projectID | (RTI2018-094564-B-100) | |
dc.relation.projectID | (P2018/NMT-4389) | |
dc.relation.projectID | (grant no. 307415) | |
dc.rights | Atribución 3.0 España | |
dc.rights.accessRights | open access | |
dc.rights.uri | https://creativecommons.org/licenses/by/3.0/es/ | |
dc.subject.cdu | 577.112 | |
dc.subject.keyword | Pulmonary surfactant protein C | |
dc.subject.keyword | Dimerization | |
dc.subject.ucm | Bioquímica (Biología) | |
dc.subject.unesco | 2302 Bioquímica | |
dc.title | Dimerization of the pulmonary surfactant protein C in a membrane environment | |
dc.type | journal article | |
dc.volume.number | 17 | |
dspace.entity.type | Publication | |
relation.isAuthorOfPublication | bcddc7b1-6137-48ba-921d-4abd534dfd49 | |
relation.isAuthorOfPublication.latestForDiscovery | bcddc7b1-6137-48ba-921d-4abd534dfd49 |
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