Effects of 3D nanocomposite bioceramic scaffolds on the immune response

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dc.contributor.authorCicuéndez Maroto, Mónica
dc.contributor.authorPortoles, Pilar
dc.contributor.authorMontes Casado, María
dc.contributor.authorIzquierdo Barba, Isabel
dc.contributor.authorVallet Regí, María Dulce Nombre
dc.contributor.authorPortolés Pérez, María Teresa
dc.date.accessioned2023-06-19T15:07:24Z
dc.date.available2023-06-19T15:07:24Z
dc.date.issued2014-06-14
dc.descriptionRESEARCHER ID M-3378-2014 (María Vallet Regí) ORCID 0000-0002-6104-4889 (María Vallet Regí)
dc.description.abstractThe interaction of new nanocomposite mesoporous glass/hydroxyapatite (MGHA) scaffolds with immune cells involved in both innate and acquired immunity has been studied in vitro as an essential aspect of their biocompatibility assessment. Since the immune response can be affected by the degradation products of bioresorbable scaffolds and scaffold surface changes, both processes have been evaluated. No alterations in proliferation and viability of RAW-264.7 macrophage-like cells were detected after culture on MGHA scaffolds which did not induce cell apoptosis. However, a slight cell size decrease and an intracellular calcium content increase were observed after contact of this cell line with MGHA scaffolds or their extracts. Although no changes in the percentages of RAW cells with low and high contents of reactive oxygen species (ROS) are observed by the treatment with 7 day extracts, this study has revealed modifications of these percentages after direct contact with scaffolds and by the treatment with 24 h extracts, related to the high reactivity/bioactivity of this MGHA nanocomposite at initial times. Furthermore, when normal fresh murine spleen cells were used as an experimental model closer to physiological conditions, no significant alterations in the activation of different immune cell subpopulations were detected in the presence of 24 h MGHA extract. MGHA scaffolds did not affect either the spontaneous apoptosis or intracellular cytokine expression (IL-2, IL-10, IFN-gamma, and TNF-alpha.) after 24 h treatment. The results obtained in the present study with murine immune cell subpopulations (macrophages, lymphocytes B, lymphocytes T and natural killer cells) support the biocompatibility of the MGHA material and suggest an adequate host tissue response to their scaffolds upon their implantation.
dc.description.departmentDepto. de Química en Ciencias Farmacéuticas
dc.description.facultyFac. de Farmacia
dc.description.refereedTRUE
dc.description.sponsorshipComunidad de Madrid
dc.description.sponsorshipMinisterio de Economia y Competitividad (MINECO)
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/41099
dc.identifier.doi10.1039/c4tb00106k
dc.identifier.issn2050-750X
dc.identifier.officialurlhttp://www.rsc.org/
dc.identifier.urihttps://hdl.handle.net/20.500.14352/35382
dc.issue.number22
dc.journal.titleJournal of Materials Chemistry B
dc.language.isoeng
dc.page.final3479
dc.page.initial3469
dc.publisherRoyal Society of Chemistry
dc.relation.projectIDBITI (S2009/MAT-1472)
dc.relation.projectIDMAT2012-35556
dc.relation.projectIDCS2010-11384-E
dc.relation.projectIDFIS PI10/0648
dc.rights.accessRightsopen access
dc.subject.cdu546
dc.subject.cdu615.46
dc.subject.keywordMesoporous bioactive glass
dc.subject.keywordT-cell-activation
dc.subject.keywordComposite scaffolds
dc.subject.keywordIn-vitro
dc.subject.keywordBiocompatibility
dc.subject.keywordBiomaterials
dc.subject.keywordLymphocytes
dc.subject.keywordBone
dc.subject.keywordPolarization
dc.subject.keywordOsteoblasts
dc.subject.ucmMateriales
dc.subject.ucmQuímica inorgánica (Química)
dc.subject.unesco3312 Tecnología de Materiales
dc.subject.unesco2303 Química Inorgánica
dc.titleEffects of 3D nanocomposite bioceramic scaffolds on the immune response
dc.typejournal article
dc.volume.number2
dspace.entity.typePublication
relation.isAuthorOfPublication94b23d40-3b2e-4dad-b72d-96c864251f14
relation.isAuthorOfPublicationee9272a2-db11-4efb-97f8-7ce1a18ad55e
relation.isAuthorOfPublication791023b8-2531-44eb-ba01-56e3b7caa0cb
relation.isAuthorOfPublication4b317058-0bd1-4fd8-afab-5fa79a4b7002
relation.isAuthorOfPublication.latestForDiscovery4b317058-0bd1-4fd8-afab-5fa79a4b7002
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